A5082331436- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Topological Materials and Phenomena
- Iron-based superconductors research
- Physics of Superconductivity and Magnetism
- Genomics and Chromatin Dynamics
- Phase-change materials and chalcogenides
- Cancer Immunotherapy and Biomarkers
- Graphene research and applications
- Luminescence and Fluorescent Materials
- Quantum and electron transport phenomena
- 2D Materials and Applications
- Biotin and Related Studies
- Genetics and Neurodevelopmental Disorders
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Photoreceptor and optogenetics research
- Nanocluster Synthesis and Applications
- interferon and immune responses
The Seventh Affiliated Hospital of Sun Yat-sen University
2024-2025
Sun Yat-sen University
2024-2025
Bridge University
2024
University of California, Riverside
2021-2023
Pennsylvania State University
2022-2023
Yangzhou University
2021-2022
Wuhan University
2016-2021
First Hospital of Shijiazhuang
2020
A conserved guanine-rich sequence could be a new target for anti–hepatitis C virus drug development.
5-Formylcytosine (5fC) is identified as one of the key players in active DNA demethylation and also an epigenetic mark mammals, thus representing a novel attractive target to chemical intervention. The current study represents attempt develop reversible 5fC-targeted intervention tool. A supramolecular aldehyde reactive probe was therefore introduced for selective conversion 5fC 5fC-AD nucleotide. Using various methods, we demonstrate that cucurbit[7]uril (CB7) selectively targets nucleotide...
A programmable CRISPR-Cas12a system for selective detection of various DNA glycosylases is described. By temporarily inactivating Cas12a through the introduction specific modifications in complementary strand Cas12a's crRNA, able to detect target glycosylases. This approach addresses critical gaps current diagnostics non-nucleic acid beyond limitations aptamers.
Abstract CRISPR-Cas12a technology has transformative potential, but as its applications grow, enhancing inherent functionalities is essential to meet diverse demands. Here, we reveal a regulatory mechanism for LbCas12a through direct repeat (DR) region 3′ end modifications and de-modifications, which can regulate LbCas12a’s cis- trans-cleavage activities. We extensively explored the effects of introducing phosphorylation, DNA, photo-cleavable linker, DNA at DR on functionality. find that...
5-Hydroxymethylcytosine (5hmC) plays a key role in the DNA demethylation process and serves as stable epigenetic marker human genome which is closely associated with disease progression, particularly diabetes, colorectal cancer, liver cancer. However, convenient sensitive methods for detecting quantifying 5hmC are scarce, especially complex biological environments. Herein, novel attempt at hypersensitive quantitative detection of was presented. A multifunctional photosensitive probe...
Simultaneous fluorogenic switch-on detection and single-base resolution analysis of 5fC through yielding an intramolecular cyclization nucleobase has been presented.
ConspectusNucleic acids contain a variety of different base modifications, such as decoration at the fifth position cytosine, which is one most important epigenetic modifications. Nucleic acid epigenetics mediate wide biological processes, including embryonic development and gene regulation, genomic imprinting, differentiation, X-chromosome inactivation. Furthermore, modification level can be aberrantly expressed in distinct sets tissue that indicate tumor onsets canceration. Thus, analysis...
An azide and hydrazine tethered to a naphthalimide analogue was created selectively react with 5-formyluracil in one system fluorogenically label 5-formylcytosine another system.
5-Formylcytosine (5fC) is known as one of the key players in process active DNA demethylation and displays essential epigenetic functions mammals.
Biotinylated <italic>o</italic>-phenylenediamine directly tethered to naphthalimide can both enrich and fluorogenically label 5-formyluracil in DNA under physiological conditions.
In this Letter, we establish a new theoretical paradigm for vortex Majorana physics in the recently discovered topological iron-based superconductors (TFeSCs). While TFeSCs are widely accepted as an exemplar of insulators (TIs) with intrinsic $s$-wave superconductivity, our theory implies that such common belief could be oversimplified. Our main finding is normal-state bulk Dirac nodes, usually ignored TI-based theories TFeSCs, will play key role determining state topology. particular,...
Mitochondrial DNA (mtDNA) modifications play an emerging role in innate immunity and inflammatory diseases. Nonetheless, relatively little is known regarding the locations of mtDNA modifications. Such information critically important for deciphering their roles instability, mtDNA-mediated immune responses, mitochondrial disorders. The affinity probe-based enrichment lesion-containing represents a key strategy sequencing Existing methods are limited specificity abasic (AP) sites, prevalent...
Precise control of Cas12a activity is essential for the improvement detection limit clinical diagnostics and minimization errors. This study addresses challenge controlling activity, especially in context nucleic acid where inherent incompatibility between isothermal amplification CRISPR reactions complicates accurate diagnostics. An RNA G-quadruplex (RG4) structure at 5' end crRNA introduced to modulate accurately without need chemical modifications. The results indicate that presence RG4...
A naphthalimide hydroxylamine probe has been designed and synthesized to selectively label the whole natural aldehydes present in DNAs including 5-formylcytosine, 5-formyluracil, abasic sites. The fluorescence characteristics of generated nucleosides have examined detail, reaction activities hydroxylamine, amine groups toward DNA discussed with others, which will be a vital reference for designing chemicals selective labeling DNAs.
Chemical modifications to nucleobases have a great influence on various cellular processes, by making gene regulation more complex, thus indicating their profound impact aspects of heredity, growth, and disease. Here, we provide the first genome-wide map 5-formyluracil (5fU) in living tissues evaluate potential roles for 5fU genomics. We show that an azido derivative (2-benzimidazolyl)acetonitrile has high selectivity enriching 5fU-containing genomic DNA. The results demonstrated feasibility...
Abstract In organisms 5‐formyluracil (5fU), which is known as a vital natural nucleobase, widely present. Despite the recent development of sensor designs for organic fluorescent molecules selective targeting applications, biocompatible and easily operated probe that are based on nucleobase modifications have rarely been reported. Here, we introduce idea 5fU multifunctional building block to facilitate design synthetic biosensors. The azide group was derived from sugar nucleoside, can be...
5-Formyluracil (5fU) based probes were designed and synthesized to detect Al<sup>3+</sup>ions<italic>in vitro</italic>and in biological systems.
DNA methylation is a significant epigenetic modification of the genome that involved in regulating many cellular processes. An increasing number human diseases have been discovered to be associated with aberrant methylation, and has deemed potential biomarker for such as cancers. A safe, nontoxic, sensitive method accurate detection 5-methylcytosine genomic extremely useful early diagnosis therapy In this paper, we established novel system detect 5-methylcytosine, which based on bisulfite...
5-Formyluracil (5fU) is a vital DNA marker that widely distributed in the cells of organisms. A unique feature 5fU possession potentially reactive aldehyde group its structure ...
The unusual properties of superconductivity in magic-angle twisted bilayer graphene (MATBG) have sparked enormous research interest. However, despite the dedication intensive experimental efforts and proposal several possible pairing mechanisms, origin its remains elusive. Here, using angle-resolved photoemission spectroscopy with micrometer spatial resolution, we discover replicas flat bands superconducting MATBG unaligned hexagonal boron nitride (hBN) substrate, which are absent...
Small-molecule-based G4 isolation from genomic DNA has enabled the identification of a total 51 446 PQSs (potential G-quadruplex sites).