A5067640340- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Porphyrin and Phthalocyanine Chemistry
- Cancer-related molecular mechanisms research
- Molecular Sensors and Ion Detection
- RNA Research and Splicing
- Luminescence and Fluorescent Materials
- Cancer-related gene regulation
- Photochromic and Fluorescence Chemistry
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Bioactive Compounds and Antitumor Agents
- Photodynamic Therapy Research Studies
- Cancer therapeutics and mechanisms
- Nanoplatforms for cancer theranostics
- RNA regulation and disease
- Biotin and Related Studies
- Metal complexes synthesis and properties
- melanin and skin pigmentation
- Genomics and Phylogenetic Studies
Wuhan University
2016-2025
Zhongnan Hospital of Wuhan University
2023-2025
Ministry of Education of the People's Republic of China
2024
University of Chicago
2015-2020
Howard Hughes Medical Institute
2015-2020
State Key Laboratory of Applied Organic Chemistry
2014
State Key Laboratory of Virology
2010-2013
State Key Laboratory of Natural and Biomimetic Drugs
2010-2013
Peking University
2010
Zero to Three
2008
Transcriptional regulation of circadian rhythms is essential for lipid metabolic homeostasis, disruptions which can lead to diseases. Whether N6-methyladenosine (m6A) mRNA methylation impacts metabolism unclear. Here, we show m6A oscillations in murine liver depend upon a functional clock. Hepatic deletion Bmal1 increases methylation, particularly PPaRα. Inhibition via knockdown methyltransferase METTL3 decreases PPaRα abundance and lifetime expression, reducing accumulation cells vitro....
N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic messenger RNA (mRNA). Recent discoveries of demethylases and specific binding proteins m6A as well methylomes obtained mammals, yeast plants have revealed regulatory functions this modification. Although present ribosomal bacteria, its occurrence mRNA still remains elusive. Here, we employed ultra-high pressure liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS) to...
The epigenetic modification of nucleic acids represents a versatile approach for achieving high-efficient control over gene expression and transcription could dramatically expand their biosensing therapeutic applications. Demethylase-involved removal N6-methyladenine (m6A) one the vital reprogramming events, yet its direct intracellular evaluation as-guided regulation are extremely rare. endonuclease-mimicking deoxyribozyme (DNAzyme) is catalytically active DNA that enables site-specific...
Abstract Although extensively studied in prokaryotes, the prevalence and significance of DNA N 6 -methyladenine (6mA or m dA) eukaryotes had been underappreciated until recent studies, which have demonstrated that 6mA regulates gene expression as a potential heritable mark. To interrogate sites at single-base resolution, we report DA-6mA-seq (DpnI-Assisted -methylAdenine sequencing), an approach uses DpnI to cleave methylated adenine duplex DNA. We find cuts other sequence motifs besides...
Bend and stretch…︁ bend An azobenzene derivative was used to induce reversible stretching folding of G-quadruplex DNA upon photoirradiation (see picture). The G quadruplex formed in the presence trans isomer dissociated by irradiation with UV light, resulting open oligomer refolded into a under visible light. This nanodevice thus converts light directly mechanical work. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but...
Existence of G-quadruplex DNA in vivo always attract widespread interest the field biology and biological chemistry. We reported our findings for existence structures promoter region oncogenes confirmed by cross-linking strategy. Probes selective was designed synthesized that show high selectivity cross-linking. Further studies demonstrated its good inhibition activity against murine melanoma cells. To further investigate if formed as target, a derivative pull-down process toward chromosome...
Innovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement oxygen with progressively larger atoms (sulfur and selenium) at 4-position deoxythymidine triphosphate weaken ability base pair m6A, while maintaining A-T* virtually same as natural one. 4SedTTP turned out be an outstanding candidate that endowed a specific signature RT...
CRISPR Cas13a provided a robust, simple and fluorescence-based method for m 1 A detection dynamic demethylation analysis of 28S rRNA.
Abstract The development of RNA aptamers/fluorophores system is highly desirable for understanding the dynamic molecular biology RNAs in vivo. Peppers-based imaging systems have been reported and applied mRNA living cells. However, need to insert corresponding aptamer sequences into target relatively low fluorescence signal limit its application endogenous imaging. Herein, we remolded original Pepper developed a tandem array inert (iPepper) turn-on system. iPepper allows efficient selective...
Abstract Over 150 types of chemical modifications have been identified in RNA to date, with pseudouridine (Ψ) being one the most prevalent RNA. Ψ plays vital roles various biological processes, and precise, base-resolution detection methods are fundamental for deep analysis its distribution function. In this study, we introduced a novel method named pseU-TRACE. pseU-TRACE relied on fact that containing underwent base deletion after treatment bisulfite (BS) during reverse transcription, which...
Abstract Water‐soluble, octacationic zinc phthalocyanine (ZnPc) was found to be a very good G‐quadruplex DNA stabilizer by using UV‐melting studies and polymerase stop assays, potent telomerase inhibitor the telomeric repeat amplification protocol (TRAP) assay. The compound’s DNA‐binding properties were also studied surface plasmon resonance (SPR). Furthermore, CD experiments demonstrated that ZnPc could induce intramolecular structure transition from antiparallel parallel form. More...
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS applied to testify that the probe reacted with oligodeoxynucleotide selectivity achieve detection conveniently quantificationally method of fluorescence. The feasibility fluorescently in genome was also investigated.
N6-methyldeoxyadenosine (6mA or m6dA) was shown more than 40 years ago in simple eukaryotes. Recent studies revealed the presence of 6mA prevalent eukaryotes, even vertebrates. However, functional characterizations have been limited. We use Tetrahymena thermophila as a model organism to examine effects on nucleosome positioning. Independent methods reveal enrichment near and after transcription start sites with periodic pattern anti-correlation relationship positions nucleosomes. The...
Abstract N6‐methyladenosine (m 6 A) is rapidly being studied and uncovered to play a significant role in various biological processes as well RNA fate functions, while the effects of defined m A sites are rarely characterized for lack convenient tools. To provide an applicable method remove modification at specific loci, editing system called “ t argeted R N de methylation by S unTag (TRADES)” engineered. In this system, targeting element dCas13b fused ten copies GCN4 peptides which can...
The accurate intracellular imaging of metal ions requires an exquisite site-specific activation metal-ion sensors, for which the pervasive epigenetic regulation strategy can serve as ideal alternative thanks to its orthogonal control feature and endogenous cell/tissue-specific expression pattern. Herein, a simple yet versatile demethylation was proposed on-site repairing-to-activating metal-ion-targeting DNAzyme achieving in live cells. This demethylation-regulating system prepared by...
N4-Acetylcytidine (ac4C) has been found to affect a variety of cellular and biological processes. For mechanistic understanding the roles ac4C in biology disease, we present an antibody-free, fluorine-assisted metabolic sequencing method detect RNA ac4C, called "FAM-seq". We successfully applied FAM-seq profile landscapes human 293T, HeLa, MDA cell lines parallel with reported acRIP-seq method. By comparison classic antibody method, that is convenient reliable for transcriptome-wide mapping...