A5101631387- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- RNA Research and Splicing
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Histone Deacetylase Inhibitors Research
- Bone health and treatments
- Protein Degradation and Inhibitors
- Bone fractures and treatments
- Bone and Dental Protein Studies
- Bone Metabolism and Diseases
- Protease and Inhibitor Mechanisms
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Orthopedic Surgery and Rehabilitation
- Molecular Biology Techniques and Applications
- Bone Tissue Engineering Materials
- Connective tissue disorders research
- Ubiquitin and proteasome pathways
Foshan University
2024-2025
Dalian Medical University
2025
First Affiliated Hospital of Dalian Medical University
2025
University of Pennsylvania
2019-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2020-2023
Sun Yat-sen University
2020-2023
Huaian First People’s Hospital
2017-2022
Zhejiang University
2018-2022
First Affiliated Hospital Zhejiang University
2019-2022
Nanjing Medical University
2017-2022
Inactivating mutations of the PHEX (phosphate-regulating gene with homologies to endopeptidases on X chromosome) endopeptidase, disease-causing in X-linked hypophosphatemia (XLH), results increased circulating levels fibroblastic growth factor-23 (FGF23), a bone-derived phosphaturic factor. To determine causal role FGF23 XLH, we generated combined Fgf23-deficient enhanced green fluorescent protein (eGFP) reporter and Phex-deficient Hyp mouse model (Fgf23(+/-)/Hyp). eGFP expression was...
Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of progenitors vivo and their progeny has not been precisely defined. We hypothesize that expressing a smooth muscle α-actin promoter (αSMA)-directed Cre transgene represent adult bone tissue. By combining complementary colors combination with transgenes activating at mature stages lineage, we characterized phenotype confirmed ability isolated αSMA(+) to progress from fully...
The ten-eleven translocation 1 (TET1) gene is the founding member of TET family enzymes (TET1/2/3) that convert 5-methylcytosine to 5-hydroxymethylcytosine. Although TET1 was first identified as a fusion partner mixed lineage leukemia (MLL) in acute myeloid carrying t(10,11), its definitive role unclear. In contrast frequent down-regulation (or loss-of-function mutations) and critical tumor-suppressor roles three genes observed various types cancers, here we show direct target MLL-fusion...
Bone is maintained by coupled activities of bone-forming osteoblasts/osteocytes and bone-resorbing osteoclasts. Alterations in this relationship can lead to pathologic bone loss such as osteoporosis. It well known that osteogenic cells support osteoclastogenesis via production RANKL. Interestingly, our recently identified marrow mesenchymal cell population—marrow adipogenic lineage precursors (MALPs) form a multidimensional network bone—was computationally demonstrated be the most...
Abstract MicroRNAs are subject to precise regulation and have key roles in tumorigenesis. In contrast the oncogenic role of miR-22 reported myelodysplastic syndrome (MDS) breast cancer, here we show that is an essential anti-tumour gatekeeper de novo acute myeloid leukaemia (AML) where it significantly downregulated. Forced expression suppresses leukaemic cell viability growth vitro , substantially inhibits development maintenance vivo . Mechanistically, targets multiple oncogenes, including...
Abstract Life experience can leave lasting marks, such as epigenetic changes, in the brain. How life is translated into storable information remains largely unknown. With unbiased data-driven approaches, we predicted that Egr1 , a transcription factor important for memory formation, plays an essential role brain programming. We performed EGR1 ChIP-seq and validated thousands of binding sites with methylation patterns established during postnatal development. More specifically, these become...
Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies with variable response to treatment. AMLs bearing MLL (mixed lineage leukemia) rearrangements are associated intermediate or poor survival. MicroRNAs (miRNAs), class small noncoding RNAs, have been postulated be important gene expression regulators virtually in all biological processes, including leukemogenesis. Through large-scale, genome-wide miRNA profiling assay 85 human AML and 15 normal control samples,...
There is an increasing need for efficient phenotyping and histopathology of a variety tissues. This evident with the ambitious projects to disrupt every gene in mouse genome. The research community needs rapid inexpensive means phenotype tissues via histology. Histological analyses skeletal are often time consuming semi-quantitative at best, regularly requiring subjective interpretation slides from trained individuals. Here, we present cryohistological paradigm mineralized First, novel...
MicroRNAs (miRNAs), small noncoding RNAs that regulate target gene mRNAs, are known to contribute pathogenesis of cancers. Acute myeloid leukemia (AML) is a group heterogeneous hematopoietic malignancies with various chromosomal and/or molecular abnormalities. AML translocations involving the mixed lineage ( MLL ) usually associated poor survival. In present study, through large-scale, genomewide miRNA expression assay, we show microRNA-9 (miR-9) most specifically up-regulated in MLL-...
Cells integrate mechanical cues to direct fate specification maintain tissue function and homeostasis. While disruption of these is known lead aberrant cell behavior chronic diseases, such as tendinopathies, the underlying mechanisms by which signals are not well understood. Here, we show using a model tendon de-tensioning that loss tensile in vivo acutely changes nuclear morphology, positioning, expression catabolic gene programs, resulting subsequent weakening tendon. In vitro studies...
The power for appreciating complex cellular interactions during embryonic development using green fluorescent protein (GFP) as a visual histological marker has not been applied to adult tissues due loss of GFP signal paraffin embedding and high autofluorescent background, particularly in section bone marrow. Here we demonstrate that the is well preserved frozen sections decalcified bone. Using tape-transfer system preserves relationships, expression can be related standard stains used...