A5071922281- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- Bone Metabolism and Diseases
- Head and Neck Cancer Studies
- TGF-β signaling in diseases
- Salivary Gland Disorders and Functions
- Spine and Intervertebral Disc Pathology
- Oral health in cancer treatment
- Mesenchymal stem cell research
- Bone health and osteoporosis research
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Knee injuries and reconstruction techniques
- Musculoskeletal pain and rehabilitation
- Effects of Radiation Exposure
- Hippo pathway signaling and YAP/TAZ
- Bone and Joint Diseases
- Cytokine Signaling Pathways and Interactions
- Bone fractures and treatments
- Parathyroid Disorders and Treatments
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Periodontal Regeneration and Treatments
- Immune cells in cancer
University of Pennsylvania
2016-2025
Orthopedic One
2023-2024
Chinese University of Hong Kong
2008-2024
California University of Pennsylvania
2018-2024
Xiangya Hospital Central South University
2021-2024
Central South University
2021-2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2023
Academy of Medical Sciences
2023
Philadelphia University
2019-2022
Chengdu Medical College
2022
Bone marrow mesenchymal lineage cells are a heterogeneous cell population involved in bone homeostasis and diseases such as osteoporosis. While it is long postulated that they originate from stem cells, the true identity of progenitors their vivo bifurcated differentiation routes into osteoblasts adipocytes remain poorly understood. Here, by employing large scale single transcriptome analysis, we computationally defined at different stages delineated bi-lineage paths young, adult aging mice....
Bone is maintained by coupled activities of bone-forming osteoblasts/osteocytes and bone-resorbing osteoclasts. Alterations in this relationship can lead to pathologic bone loss such as osteoporosis. It well known that osteogenic cells support osteoclastogenesis via production RANKL. Interestingly, our recently identified marrow mesenchymal cell population—marrow adipogenic lineage precursors (MALPs) form a multidimensional network bone—was computationally demonstrated be the most...
Significance Cartilage is essential in vertebrate development and adulthood. growth plates ensure skeletal until closing at puberty, articular cartilage ensures lifelong structural functional integrity of joints. Chondrocytes build development, governed by the transcription factor SOX9. Using mouse models transcriptome profiling approaches, we show here that SOX9 also has key roles to maintain open postnatally protect adult from osteoarthritic degradation. In particular, safeguards lineage...
EGFR activation by overexpressing HBEGF in cartilage or intra-articular delivery of TGFα nanoparticles attenuates osteoarthritis development mice.
Abstract In studies of electron and proton radiotherapy, ultrahigh dose rates FLASH radiotherapy appear to produce fewer toxicities than standard while maintaining local tumor control. FLASH-proton (F-PRT) brings the spatial advantages PRT (>40 Gy/second), making it important understand if how F-PRT spares normal tissues providing antitumor efficacy that is equivalent standard-proton (S-PRT). Here we studied damage skin mesenchymal muscle bone found C57BL/6 murine hind leg produced...
Non-hematopoietic cells are essential contributors to hematopoiesis. However, heterogeneity and spatial organization of these in human bone marrow remain largely uncharacterized. We used single-cell RNA sequencing (scRNA-seq) profile 29,325 non-hematopoietic discovered nine transcriptionally distinct subtypes. simultaneously profiled 53,417 hematopoietic predicted their interactions with subsets. employed co-detection by indexing (CODEX) spatially over 1.2 million cells. integrated scRNA-seq...
The infrapatellar fat pad (IPFP) and synovium play essential roles in maintaining knee joint homeostasis the progression of osteoarthritis (OA). cellular transcriptional mechanisms regulating function these specialized tissues under healthy diseased conditions are largely unknown. Here, single-cell single-nuclei RNA sequencing human IPFP synovial were performed to elucidate composition profile. Computational trajectory analysis revealed that dipeptidyl peptidase 4 + mesenchymal cells as a...
The clinical findings that alendronate blunted the anabolic effect of human parathyroid hormone (PTH) on bone formation suggest active resorption is involved and enhances effect. PTH signals via its receptor osteoblast membrane, osteoclasts are impacted indirectly products osteoblasts. Microarray with RNA from rats injected or vehicle showed a strong association between stimulation monocyte chemoattractant protein-1 (MCP-1) effects PTH. rapidly dramatically stimulated MCP-1 mRNA in femora...
Osteosarcoma is the most common type of solid bone cancer and second leading cause cancer-related death in pediatric patients. Many patients are not cured by current osteosarcoma therapy consisting combination chemotherapy along with surgery thus new treatments urgently needed. In last decade, stem cells have been identified many tumors such as leukemia, brain, breast, head neck, colon, skin, pancreatic, prostate cancers these proposed to play major roles drug resistance, tumor recurrence,...
Therapeutic resistance remains a persistent challenge for patients with malignant tumors. Here, we reveal that endothelial cells (ECs) acquire transformation into mesenchymal stem cell (MSC)-like in glioblastoma (GBM), driving tumor to cytotoxic treatment. Transcriptome analysis by RNA sequencing (RNA-seq) revealed ECs undergo and stemness-like activation GBM microenvironment. Furthermore, identified c-Met-mediated axis induces β-catenin phosphorylation at Ser675 Wnt signaling activation,...
Focal radiotherapy is frequently associated with skeletal damage within the radiation field. Our previous in vitro study showed that activation of Wnt/β-catenin pathway can overcome radiation-induced DNA and apoptosis osteoblastic cells. Neutralization circulating sclerostin a monoclonal antibody (Scl-Ab) an innovative approach for treating osteoporosis by enhancing signaling bone. Together fact focal increases amount bone, we sought to determine whether weekly treatment Scl-Ab would prevent...
Significance The uppermost superficial zone of articular cartilage plays multifaceted roles in maintaining structure, function, and mechanical properties preventing degeneration during osteoarthritis initiation. However, its regulation by growth factors hormones is still largely unknown. Here we report that EGFR signaling an important factor pathway maintains chondrocyte number, promotes boundary lubricant secretion surface lubrication, stimulates strength cartilage. Reduction activity leads...
Joint biomechanical functions rely on the integrity of cartilage extracellular matrix. Understanding molecular activities that govern matrix assembly is critical for developing effective regeneration strategies. This study elucidated role decorin, a small leucine-rich proteoglycan, in structure and cartilage. In decorin-null cartilage, we discovered substantial reduction aggrecan content, major proteoglycan matrix, mild changes collagen fibril nanostructure. loss resulted significantly...
Bone fragility fractures are caused by low bone mass or impaired quality. Osteoblast/osteoclast coordination determines mass, but the factors that control quality poorly understood. Osteocytes regulate osteoblast and osteoclast activity on surfaces can also directly reorganize matrix to improve through perilacunar/canalicular remodeling; however, molecular mechanisms remain unclear. We previously found deleting transcriptional regulators Yes-associated protein (YAP) co-activator with...
The engineered nanoparticles mitigate osteoarthritis progression.
Colony-stimulating factor 1 (Csf1) is an essential growth for osteoclast progenitors and important regulator bone resorption. It remains elusive which mesenchymal cells synthesize Csf1 to stimulate osteoclastogenesis. We recently identified a novel cell population, marrow adipogenic lineage precursors (MALPs), in bone. Compared other subpopulations, MALPs expressed at much higher level this expression was further increased during aging. To investigate its role, we constructed MALP-deficient...
Osteoarthritis (OA) affects multiple tissues in the knee joint, including synovium and intra-articular adipose tissue (IAAT) that are attached to each other. However, whether these two share same progenitor cells hence function as a single unit joint homeostasis diseases is largely unknown. Single-cell transcriptomic profiling of infrapatellar fat pad (IFP), largest IAAT, from control OA mice revealed five mesenchymal clusters predicted (MPCs) common progenitors for other cells: synovial...
Parathyroid hormone (PTH) binds to its receptor PTH1R (parathyroid 1 receptor) in osteoblastic cells regulate bone remodeling and calcium homeostasis. While prolonged exposure PTH causes increased resorption, intermittent injections of have an anabolic effect on bone. The molecular mechanisms regulating these processes are still largely unknown. Here, we present our results gene expression profile changes the PTH-treated cell line, UMR 106-01, using DNA microarray analysis. A total 125 known...
Abstract The epidermal growth factor receptor (EGFR) and its ligands regulate key processes of cell biology, such as proliferation, survival, differentiation, migration, tumorigenesis. We previously showed that, EGFR signaling pathway is an important bone regulator it primarily plays anabolic role in metabolism. In this study, we demonstrated that EGF‐like strongly inhibited osteoblast differentiation mineralization several lines osteoblastic cells. Real‐time RT‐PCR promoter reporter assays...