A5072987059- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Photoacoustic and Ultrasonic Imaging
- Head and Neck Cancer Studies
- Salivary Gland Disorders and Functions
- Oral health in cancer treatment
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Effects of Radiation Exposure
- Sarcoma Diagnosis and Treatment
- Optical Imaging and Spectroscopy Techniques
- Radiation Effects in Electronics
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Medical Imaging and Pathology Studies
- Medical and Biological Ozone Research
- Radiation Detection and Scintillator Technologies
- Virus-based gene therapy research
- Immune cells in cancer
- Pleural and Pulmonary Diseases
- Occupational and environmental lung diseases
University of Pennsylvania
2016-2025
California University of Pennsylvania
2013-2024
Hospital of the University of Pennsylvania
2020-2023
University of Pennsylvania Health System
2015-2018
California Environmental Protection Agency
2009
University of California, Berkeley
2009
California Department of Toxic Substances Control
2009
PurposeRecent studies suggest that ultrahigh-dose-rate, "FLASH," electron radiation therapy (RT) decreases normal tissue damage while maintaining tumor response compared with conventional dose rate RT. Here, we describe a novel RT apparatus delivers FLASH proton (PRT) using double scattered protons computed tomography guidance and provide the first report of RT-mediated radioprotection.Methods MaterialsAbsolute was measured at multiple depths in solid water validated against an absolute...
Abstract In studies of electron and proton radiotherapy, ultrahigh dose rates FLASH radiotherapy appear to produce fewer toxicities than standard while maintaining local tumor control. FLASH-proton (F-PRT) brings the spatial advantages PRT (>40 Gy/second), making it important understand if how F-PRT spares normal tissues providing antitumor efficacy that is equivalent standard-proton (S-PRT). Here we studied damage skin mesenchymal muscle bone found C57BL/6 murine hind leg produced...
Ultra-high dose rate FLASH proton radiotherapy (F-PRT) has been shown to reduce normal tissue toxicity compared standard (S-PRT) in experiments using the entrance portion of depth profile, while therapy uses a spread-out Bragg peak (SOBP) with unknown effects on sparing. To investigate, biological F-PRT an SOBP and region were S-PRT mouse intestine. In this study, 8–10-week-old C57BL/6J mice underwent 15 Gy (absorbed dose) whole abdomen irradiation four groups: (1) F-PRT, (2) S-PRT, (3) (4)...
Head and neck cancer radiotherapy often damages salivary glands oral mucosa, severely negatively impacting patients' quality of life. The ability FLASH proton (F-PRT) to decrease normal tissue toxicity while maintaining tumor control compared with standard (S-PRT) has been previously demonstrated for several tissues. However, its potential in ameliorating radiation-induced gland dysfunction mucositis controlling orthotopic head growth not reported. area C57BL/6 mice was irradiated a single...
High levels (μg/g lw) of polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) were measured in peregrine falcon eggs from California (n = 90 52 birds, 38 nest sites, collected 1986−2007, ΣPBDEs median 4.53, range 0.08−53.1). Over the past 22 years, PBDE more than tripled each decade eggs, whereas PCB had no significant changes. highest major cities ("Big Cities"), PCBs showed difference across regions. For PBDEs, Big City markedly different patterns Coastal eggs:...
Dosimetry of singlet oxygen (1O2) is particular interest because it the major cytotoxic agent causing biological effects for type-II photosensitizers during photodynamic therapy (PDT). An in-vivo model to determine threshold dose, [1O2]rx,sh, PDT was developed.An radiation-induced fibrosarcoma (RIF) tumor mouse used correlate radius necrosis calculation based on explicit dosimetry light fluence distribution, tissue optical properties, and photosensitizer concentrations. Inputs include five...
Abstract 3D patient-derived organoids (PDOs) have been utilized to evaluate potential therapies for patients with different cancers. However, the use of PDOs created from treatment-naive patient biopsies prediction clinical outcomes in esophageal cancer has not yet reported. Herein we describe a pilot prospective observational study goal determining whether treatment naive can model or predict outcomes. Endoscopic at single tertiary care center were used generate PDOs, which treated...
Research efforts in FLASH radiotherapy have increased at an accelerated pace recently. involves ultra-high dose rates and has shown to reduce toxicity normal tissue while maintaining tumor response pre-clinical studies when compared conventional rate radiotherapy. The goal of this review is summarize the performed to-date with proton, electron, heavy ion radiotherapy, particular emphasis on physical aspects each study advantages disadvantages modality. Beam delivery parameters, experimental...
FLASH is a high-dose-rate form of radiation therapy that has the reported ability, compared with conventional dose rates, to spare normal tissues while being equipotent in tumor control, thereby increasing therapeutic ratio. The mechanism underlying this tissue sparing effect currently unknown, however one possibility radiochemical oxygen depletion (ROD) during delivery at rates. In order investigate possibility, we used phosphorescence quenching method measure partial pressure before, and...
Pancreatic ductal adenocarcinoma (PDAC) represents a challenge in oncology, with limited treatment options for advanced-stage patients. Chimeric antigen receptor T cell (CAR T) therapy targeting mesothelin (MSLN) shows promise, but challenges such as the hostile immunosuppressive tumor microenvironment (TME) hinder its efficacy. This study explores synergistic potential of combining proton radiation (RT) MSLN-targeting CAR syngeneic PDAC model. Proton RT significantly increased MSLN...
Although photodynamic therapy (PDT) is an established modality for cancer treatment, current dosimetric quantities, such as light fluence and PDT dose, do not account the differences in oxygen consumption different rates (ϕ). A macroscopic model was adopted to evaluate using calculated reacted singlet concentration ([O21]rx) predict Photofrin-PDT outcome mice bearing radiation-induced fibrosarcoma tumors, primary cytotoxic species responsible cell death type II PDT. Using a combination of...
Type II photodynamic therapy (PDT) is based on the photochemical reactions mediated through an interaction between a photosensitizer, ground-state oxygen ([(3)O2]), and light excitation at appropriate wavelength, which results in production of reactive singlet ([(1)O2]rx). We use empirical macroscopic model four parameters for calculation [(1)O2]rx threshold concentration ([(1)O2]rx,sh) causing tissue necrosis tumors after PDT. For this reason, 2-(1-hexyloxyethyl)-2-devinyl...
Allogeneic invariant natural killer T cells (allo-iNKTs) induce clinical remission in patients with otherwise incurable cancers and COVID-19-related acute respiratory failure. However, their functionality is inconsistent among individuals, they become rapidly undetectable after infusion, raising concerns over rejection limited therapeutic potential. We validate a strategy to promote allo-iNKT persistence dogs, an established large-animal model for novel cellular therapies. identify...
Photosensitizer fluorescence excited by photodynamic therapy (PDT) treatment light can be used to monitor the in vivo concentration of photosensitizer and its photobleaching. The temporal integral product fluence is called PDT dose, which an important dosimetry quantity for PDT. However, detected may distorted variations absorption scattering both excitation tissue. Therefore, correction measured distortion due variable optical properties required absolute quantification concentration. In...
Abstract Photodynamic therapy (PDT) has shown promise as an adjuvant treatment for malignant pleural mesothelioma when combined with surgical resection. Accurate light dosimetry is critical efficacy. This study presents improved method analyzing fluence distribution in PDT using a standardized anatomical coordinate system and advanced computational modeling. We utilized infrared navigation delivery wand to track real‐time. The human chest cavity geometry was reconstructed the pleura mapped...
Abstract The effect of FLASH Proton Radiation therapy (F-PRT) in decreasing normal tissue toxicity while maintaining equal tumor control compared to Standard (S-PRT) has been previously investigated for head and neck tissues mice using a single dose either S-PRT/F-PRT. Here, we studied the F-PRT following hypo-fractionated regimen re-irradiation setting. area C57Bl/6 was irradiated with 12 Gy (128Gy/s) or S-PRT (0.95 Gy/s) followed scheme (6 x 3). Mice orthotopic tongue tumors were also two...
Ultra-high dose rate radiotherapy elicits a biological effect (FLASH), which has been shown to reduce toxicity while maintaining tumor control in preclinical radiobiology experiments. FLASH depends on the rate, with evidence that higher rates drive increased normal tissue sparing. The pattern of delivery also significance for conformal proton delivered via pencil beam scanning (PBS) given its unique spatio-temporal distribution deposition. In PBS, machine-generated log file contains...