A5085389686- Renal and related cancers
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Testicular diseases and treatments
- Single-cell and spatial transcriptomics
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Toxoplasma gondii Research Studies
- CRISPR and Genetic Engineering
- Nanoparticle-Based Drug Delivery
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- Nanoplatforms for cancer theranostics
- Parasitic Infections and Diagnostics
- Birth, Development, and Health
- Supramolecular Chemistry and Complexes
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Prenatal Screening and Diagnostics
University of Pennsylvania
2019-2025
California University of Pennsylvania
2024
Old Dominion University
2016
Abstract Objectives Fibroblast growth factor 9 (FGF9) is expressed by somatic cells in the seminiferous tubules, yet little information exists about its role regulating spermatogonial stem (SSCs). Materials and Methods Fgf9 overexpression lentivirus was injected into mouse testes, PLZF immunostaining performed to investigate effect of FGF9 on spermatogonia vivo. Effect SSCs detected transplanting cultured germ tubules testes. RNA‐seq bulk RNA single cell explore working mechanisms. SB203580...
Allogeneic invariant natural killer T cells (allo-iNKTs) induce clinical remission in patients with otherwise incurable cancers and COVID-19-related acute respiratory failure. However, their functionality is inconsistent among individuals, they become rapidly undetectable after infusion, raising concerns over rejection limited therapeutic potential. We validate a strategy to promote allo-iNKT persistence dogs, an established large-animal model for novel cellular therapies. identify...
Treatment of cancer in children is increasingly successful but leaves many prepubertal boys suffering from infertility or subfertility later life. A current strategy to preserve fertility these cryopreserve a testicular biopsy prior treatment with the expectation future technologies allowing for reintroduction stem cells and restoration spermatogenesis. Spermatogonial (SSCs) form basis male reproduction, differentiating into all germ cell types, including mature spermatozoa can regenerate...
The adrenal cortex produces essential steroid hormones through a concentric zonal architecture, established by the centripetal trans-differentiation of subcapsular progenitors within capsule-derived niche. To capture this complexity, we establish human pluripotent stem cell-derived organoid system that faithfully recapitulates process. RSPO3/WNT signaling from capsule specifies definitive zone (DZ) primordium, which then differentiate into cortisol-producing transitional and an...
Abstract Retinoic acid (RA) induces spermatogonial differentiation, but the mechanism by which it operates remains largely unknown. We developed a germ cell culture assay system to study genes involved in differentiation triggered RA. Stimulated RA 8 (Stra8), RA-inducible gene, is indispensable for meiosis initiation, and its deletion results complete block of spermatogenesis at pre-leptotene/zygotene stage. To interrogate role Stra8 mediated spermatogonia, we derived cultures from neonatal...
Summary Aberrant male germline development can lead to the formation of seminoma, a testicular germ cell tumor. Seminomas are biologically similar primordial cells (PGCs) and many bear an isochromosome 12p [i(12p)] with two additional copies short arm chromosome 12. By mapping seminoma transcriptomes open chromatin landscape onto normal human trajectory, we find that resembles premigratory/migratory cells, but exhibit enhanced pluripotency programs, upregulation genes involved in apoptosis,...
Abstract Failures in germline development drive male infertility, but the lack of model systems that recapitulate human spermatogenesis hampers therapeutic development. Here, we have developed a system to differentiate induced pluripotent stem cells (iPSCs) into primordial germ cell-like self-organize within xenogeneic reconstituted testes (xrTestes) generated from mouse fetal testicular cells. Subsequent transplant xrTestes immunodeficient mice resulted efficient generation undifferentiated...
Aberrant male germline development can lead to the formation of seminoma, a testicular germ cell tumor. Seminomas are biologically similar primordial cells (PGCs) and many bear an isochromosome 12p [i(12p)] with two additional copies short arm chromosome 12. By mapping seminoma transcriptomes open chromatin landscape onto normal human trajectory, we find that resembles premigratory/migratory PGCs; however, it exhibits enhanced pluripotency programs upregulation genes involved in apoptosis,...
Nanomaterial based imaging approaches hold substantial promise in addressing current diagnostic and therapeutic challenges. One of the key requirements for successful clinical translation nanomaterials is their complete clearance from body within a reasonable time period preferably via renal filtration route. This article describes synthesis highly fluorescent, water soluble, resorcinarene cavitand nanocapsules demonstrates effective mice. The functionalization was accomplished one-pot...
Motivation: As the mean age of parenthood grows, effect parental on genetic disease and child health becomes ever more important. A number autosomal dominant disorders show a dramatic paternal due to selfish mutations: substitutions that grant spermatogonial stem cells (SSCs) selective advantage in testes father, but have deleterious offspring. In this paper we present computational technique model SSC niche order examine phenomenon draw conclusions across different genes disorders. Results:...