A5101988840- Osteoarthritis Treatment and Mechanisms
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Advanced Photocatalysis Techniques
- Knee injuries and reconstruction techniques
- Bone health and treatments
- Shoulder Injury and Treatment
- Bone and Joint Diseases
- Plant Toxicity and Pharmacological Properties
- Orthopaedic implants and arthroplasty
- Advanced Nanomaterials in Catalysis
- Pulmonary Hypertension Research and Treatments
- Bee Products Chemical Analysis
- Vitamin D Research Studies
- Nanocluster Synthesis and Applications
- Cancer-related Molecular Pathways
- Bioactive Natural Diterpenoids Research
- Developmental Biology and Gene Regulation
- Tendon Structure and Treatment
- Phytochemistry and Bioactivity Studies
- Hip disorders and treatments
- Pharmacological Effects of Natural Compounds
- Circadian rhythm and melatonin
- Inflammatory mediators and NSAID effects
- Spinal Hematomas and Complications
Xi'an Honghui Hospital
2022-2024
Xi'an Jiaotong University
2022-2024
Air Force Medical University
2020-2023
Xijing Hospital
2020-2023
Changzhou University
2023
Xi'an Medical University
2023
Shihezi University
2016-2023
Sixth Affiliated Hospital of Xinjiang Medical University
2023
Xinjiang Medical University
2023
Northwest University
2023
Significance The uppermost superficial zone of articular cartilage plays multifaceted roles in maintaining structure, function, and mechanical properties preventing degeneration during osteoarthritis initiation. However, its regulation by growth factors hormones is still largely unknown. Here we report that EGFR signaling an important factor pathway maintains chondrocyte number, promotes boundary lubricant secretion surface lubrication, stimulates strength cartilage. Reduction activity leads...
Intervertebral disc degeneration (IDD) has been generally accepted as the major cause of low back pain (LBP), which causes an enormous socioeconomic burden. Previous studies demonstrated that apoptosis nucleus pulposus (NP) cells and dyshomeostasis extracellular matrix (ECM) contributed to pathogenesis IDD, effective therapies were still lacking. Quercetin, a natural flavonoid possessing specific effect autophagy stimulation SIRT1 activation, showed some protective on series degenerative...
Abstract Mitochondrial dysfunction plays a major role in the development of intervertebral disc degeneration (IDD). Sirtuin 5 (SIRT5) participates maintenance mitochondrial homeostasis through its desuccinylase activity. However, it is still unclear whether succinylation or SIRT5 involved impairment mitochondria and IDD induced by excessive mechanical stress. Our 4D label-free quantitative proteomic results showed decreased expression rat nucleus pulposus (NP) tissues under loading....
Excessive mechanical load (overloading) is a well-documented pathogenetic factor for many mechano stress-induced pathologies, i.e. intervertebral disc degeneration (IDD). Under overloading, the balance between anabolism and catabolism within nucleus pulposus (NP) cells are badly thrown off, NP undergo apoptosis. However, little known about how overloading transduced to contributes degeneration. The current study shows that conditional knockout of Krt8 (keratin 8) aggravates load-induced IDD...
To establish an unbiased, 3-dimensional (3-D) approach that quantifies subchondral bone plate (SBP) changes in mouse joints, and to investigate the mechanism mediates SBP sclerosis at a late stage of osteoarthritis (OA).A new micro-computed tomography (micro-CT) protocol was developed characterize entire thickness distal femur normal knee. Four models severe joint OA were generated: cartilage-specific Egfr-knockout (Egfr-CKO) mice 2 months after surgical destabilization medial meniscus...
Focal radiotherapy for cancer patients has detrimental effects on bones within the radiation field and primary clinical signs of bone damage include loss functional osteoblasts. We reported previously that daily injection parathyroid hormone (PTH, 1-34) alleviates radiation-induced osteopenia in a preclinical model by improving osteoblast survival. To elucidate molecular mechanisms, we irradiated osteoblastic UMR 106-01 cells calvarial organ culture demonstrated an anti-apoptosis effect...
Low back pain is one of the most serious public health problems worldwide and major clinical manifestation intervertebral disc degeneration (IVDD). The key pathological change during IVDD dysfunction annulus fibrosus (AF). However, due to lack an in-depth understanding AF biology, methods reconstruct are very limited. In this study, mice cell atlas were decoded by single-cell RNA sequencing provide a guide for reconstruction. results first identify new population cells, fibrochondrocyte-like...
The circadian clock participates in maintaining homeostasis peripheral tissues, including intervertebral discs (IVDs). Abnormal mechanical loading is a known risk factor for disc degeneration (IDD). Based on the rhythmic daily pattern of rest and activity, we hypothesized that abnormal could dampen IVD clock, contributing to IDD. Here, investigated effects aimed inhibit compression-induced IDD by targeting core molecule brain muscle Arnt-like protein-1 (BMAL1). In this study, showed BMAL1 KO...
Intervertebral disc degeneration is a complex disease involving genetic and environmental factors multiple cellular processes. The role expression of the lncRNA NEAT1 were assessed in intervertebral degeneration. was degenerative control nucleus pulposus using RT-PCR. Western blotting RT-PCR also used to investigate p53 p21 levels tissues. function cells with gain- loss-of-function experiments. ERK/MAPK signaling examined. NEAT1, p53, dramatically upregulated Furthermore, catabolic MMP13...
Abstract Long bone development involves the embryonic formation of a primary ossification center (POC) in incipient diaphysis followed by postnatal secondary (SOC) at each epiphysis. Studies have elucidated major basic mechanisms POC development, but relatively little is known about SOC development. To gain insights into formation, we used Col2-Cre Rosa-tdTomato (Col2/Tomato) reporter mice and found that their periarticular region contained numerous Tomato-positive lineage cells expressing...
Spinal cord injury (SCI)-induced bone loss represents the most severe osteoporosis with no effective treatment. Past animal studies have focused primarily on long bones at acute stage using adolescent rodents. To mimic chronic SCI in human patients, we performed a comprehensive analysis of long-term structural and mechanical changes axial appendicular adult rats after SCI. In this experiment, 4-month-old Fischer 344 male received clinically relevant T13 contusion injury. Sixteen weeks later,...
Abstract Background Intervertebral disc (IVD) is a highly rhythmic tissue, which experiences diurnal cycle of high/low mechanical loading via the changes activity/rest phase. There are signs that disruption peripheral IVD clock related to process intervertebral degeneration (IDD). However, it still unclear whether inflammation could disturb and thus induce IDD. Methods results In this study, we used IL-1β, commonly inflammatory factor, IDD found was dampened in degenerated human nucleus...
Cyclic GMP (cGMP) is an important intracellular regulator of endochondral bone growth and skeletal remodeling. Tadalafil, inhibitor the phosphodiesterase (PDE) type 5 (PDE5) that specifically hydrolyzes cGMP, increasingly used to treat children with pulmonary arterial hypertension (PAH), but effect tadalafil on strength has not been previously investigated. In this study, we first analyzed expression transcripts encoding PDEs in primary cultures chondrocytes from newborn rat epiphyses. We...
Osteoporosis is a polygenic disorder and has been demonstrated to be associated with ~30 candidate genes, the majority of which have also implicated in regulation bone mineral density (BMD). Vitamin D receptor (VDR) gene that most extensively studied. Certain studies reported VDR single nucleotide polymorphism ApaI risk osteoporosis Caucasian African women. However, this association not yet studied postmenopausal Han Chinese women Xinjiang area. In present study, polymorphisms were defined...
Abstract Objective This study aims to emphasize the initiating role of facet joint (FJ) degeneration in process cervical spine induced by tangential load, and we further validate it a novel animal model. Methods The characteristics patients different ages were summarized through case collection. In rat models, Hematoxylin–Eosin, Safranin O staining, micro‐computed tomography used show histopathological changes bone fiber structure FJ height intervertebral disc (IVD) space. ingrowth...
To explore the characteristics, mechanism, treatment, and prognosis of head-neck separation type Monteggia equivalent fractures in children. Patients with this injury were reviewed retrospectively. The lesion was characterized by a fracture ulnar radial neck but without dislocation head. Our classification based on direction displacement angulation radiographs, divided into extension-valgus flexion-varus type. treated reduction internal fixation, depending clinical results evaluated using...
Abstract Resistant organism caused by overuse of antibiotics brings endless resistance in wound infections treatment. As a new generation antibacterial strategy, which inspired the irreversible oxidative damage to bacteria reactive oxygen species (ROS), chemodynamics therapy has drawn considerable attention recent years. However, chemodynamics-mediate effect is restricted owing near-neutral pH and insufficient H 2 O concentration at infection site, addition exogenous would cause serious side...
Abstract Objective This study aimed to compare the distal femoral epiphyseal stress of Leg Length Discrepancy and Pelvic Tilt, explain phenomenon genu varum valgum in children with unequal lower extremities or pelvic tilt. Methods The finite element models was established analyze Equivalent Von Mises Stress, We reconstructed right sacrum, pubis, ischium, femur a healthy child by method, generated epiphysis other cartilage structures. left amputated (1, 2, 3, 4, 5, 6cm) changes bilateral...