A5100440127- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Plant Molecular Biology Research
- Biotin and Related Studies
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Biochemical and Structural Characterization
- Heat shock proteins research
- Click Chemistry and Applications
- Phytochemicals and Antioxidant Activities
- Skin and Cellular Biology Research
- Lysosomal Storage Disorders Research
- melanin and skin pigmentation
- Tea Polyphenols and Effects
- Genomics, phytochemicals, and oxidative stress
- Skin Protection and Aging
- Polyamine Metabolism and Applications
- Advanced Fluorescence Microscopy Techniques
- Insect and Pesticide Research
Shanghai Ocean University
2025
VIB-UGent Center for Plant Systems Biology
2019-2023
Ghent University
2019-2023
Tobacco Research Institute
2023
Chinese Academy of Agricultural Sciences
2023
Chinese PLA General Hospital
2023
Air Force Medical University
2023
Xijing Hospital
2023
South China Agricultural University
2014-2022
Max Planck Institute of Molecular Cell Biology and Genetics
2021
Proximity labeling is a powerful approach for detecting protein-protein interactions. Most proximity techniques use promiscuous biotin ligase or peroxidase fused to protein of interest, enabling the covalent proteins and subsequent capture identification interacting neighboring without need complex remain intact. To date, only few studies have reported on in plants. Here, we present results systematic study applying variety biotin-based approaches several plant systems using various...
Abstract The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both homologues yeast ARP2/3 activator, Pan1p. Here, we show that these also involved in actin cytoskeleton regulated autophagy. AtEH/Pan1 localise to plasma membrane autophagosomes. Upon induction autophagy, recruit TPC AP-2 subunits, clathrin, Increased expression boosts autophagosome formation, suggesting independent redundant pathways...
Aberrant liquid-to-solid phase transitions of biomolecular condensates have been linked to various neurodegenerative diseases. However, the underlying molecular interactions that drive aging remain enigmatic. Here, we develop quantitative time-resolved crosslinking mass spectrometry monitor protein and dynamics inside formed by fused in sarcoma (FUS). We identify misfolding RNA recognition motif FUS as a key driver condensate aging. demonstrate small heat shock HspB8 partitions into via its...
Excessive mechanical load (overloading) is a well-documented pathogenetic factor for many mechano stress-induced pathologies, i.e. intervertebral disc degeneration (IDD). Under overloading, the balance between anabolism and catabolism within nucleus pulposus (NP) cells are badly thrown off, NP undergo apoptosis. However, little known about how overloading transduced to contributes degeneration. The current study shows that conditional knockout of Krt8 (keratin 8) aggravates load-induced IDD...
Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into is lacking. Here, we reveal molecular architecture of plant [TPLATE (TPC)] using integrative approach. We identify crucial roles for specific subunits in assembly interaction. Our data therefore generate fresh...
Significance Endocytosis transports cargos inside the cell by creating spherical vesicles from plasma membrane. This membrane remodeling requires proteins to generate force bend inwards, overcoming high-turgor pressure in plant cells. However, as plants create clathrin-coated without actin, machinery membranes during endocytosis is entirely unknown and appears distinct other model systems. Here, we refine physiological role of plant-specific essential endocytic TPLATE complex. We find it...
Abstract Programmed cell death (PCD) is a fundamental cellular process crucial to development, homeostasis, and immunity in multicellular eukaryotes. In contrast our knowledge on the regulation of diverse animal subroutines, information execution PCD plants remains fragmentary. Here, we make use accessibility Arabidopsis (Arabidopsis thaliana) root cap visualize developmentally controlled PCD. We identify succession selective decompartmentalization events ion fluxes as part terminal...
The TPLATE complex (TPC) is a key endocytic adaptor protein in plants. TPC Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific (AtEH1/Pan1 AtEH2/Pan1) which, although cytoplasmic proteins, are not associated with the hexameric subcomplex cytoplasm. To investigate dynamic assembly of octameric at plasma membrane (PM), we performed state-of-the-art dual-color live-cell imaging physiological lowered temperatures. Lowering temperature slowed...
Significance Endocytosis controls the proteome and lipidome of plasma membrane therefore communication between cell outside world. Because its importance, many attempts have been made to develop tools interfere with this process. These include genetic approaches small chemicals. Thus far, however, developed modulate plant endocytosis either lack specificity, are irreversible, or require several days produce an effect. Here, we generated a conditional reversible tool specifically endocytosis....
Six monosaccharide derivatives of rotenone were designed and synthesized to assess whether could become phloem mobile by the addition a group. Phloem mobility experiments showed that only D-glucose conjugates exhibit transport properties in castor bean (Ricinus communis L.) seedlings. Two conjugates, 2-O-β-D-glucopyranosyldemethylrotenone 6'-O-β-D-glucopyranosyldalpanol, had significantly obtained systemicity compared with rotenone, 6'-O-β-D-glucopyranosyldalpanol was more than...
In our previous work, a glucose-fipronil (GTF) conjugate at the C-1 position was synthesized via click chemistry and glucose moiety converted non-phloem-mobile insecticide fipronil into moderately phloem-mobile insecticide. present paper, introduced C-2, C-3, C-4, C-6 positions of to obtain four new conjugates evaluate effects different isomers on phloem mobility. The mobility synthetic GTF tested using Ricinus seedling system. results confirmed that conjugation has significant influence conjugates.
The ability to visualize the movement of glycosyl insecticides contributes learning their translocation and distribution in plants. In our present work, a novel fluorescent glucose–fipronil conjugate N-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazol-5-yl]-1-(2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-β-d-glucopyranosyl)-1H-1,2,3-triazole-4-methanamine (2-NBDGTF), was obtained via click chemistry. Disk uptake experiments showed that an...
Numerous studies indicate a role for the actin cytoskeleton in secretion. Here, we have used evanescent wave and widefield fluorescence microscopy to study involvement of secretion from PC12 cells. Secretion was assayed as loss ANF-EmGFP mode. Under control conditions, depolarization induced showed two phases: an initial rapid rate vesicular cargo (tau = 1.4 s), followed by slower, sustained drop 34.1 s). Pretreatment with Latrunculin A changed kinetics single exponential, slightly faster...
Abstract Proximity-dependent biotin labelling (PDL) uses a promiscuous ligase (PBL) or peroxidase fused to protein of interest. This enables covalent proteins and allows subsequent capture identification interacting neighbouring without the need for complex remain intact. To date, only few papers report on use PDL in plants. Here we present results systematic study applying variety approaches several plant systems using various conditions bait proteins. We show that TurboID is most variant...
Abstract Calsenilin is a member of the recoverin branch EF‐hand superfamily that reported to interact with presenilins, regulate prodynorphin gene expression, modulate voltage‐gated Kv4 potassium channel function, and bind neurotoxins. Ca +2 ‐binding protein plays an important role in calcium signaling. Despite its importance numerous neurological functions, structure this has not been reported. In absence , limited spectral resolution increases upon addition . Here, we describe...
Plant essential oils restrained the course of foraging behavior individual worker ants and also influenced recruitment food transport. Worker search times increased significantly among plant oil treatments. The longest time was observed for treated with Capsicum annuum L. (Solanales: Solanaceae) Cedrus deodara (Roxb.) G.Don (Pinales: Pinaceae). Different have different effects on workers, amount transported, spent foraging.
Abstract Programmed cell death (PCD) is a fundamental cellular process crucial to development, homeostasis, and immunity in multicellular eukaryotes. In contrast our knowledge on the regulation of diverse animal subroutines, information execution PCD plants remains fragmentary. Here we make use accessibility Arabidopsis thaliana root cap visualize developmentally controlled PCD. We identify succession selective decompartmentalization events ion fluxes that are by gene regulatory network...