A5071745662- Plant Molecular Biology Research
- Plant Reproductive Biology
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Metalloenzymes and iron-sulfur proteins
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- DNA Repair Mechanisms
- Polysaccharides and Plant Cell Walls
- Biotin and Related Studies
- DNA and Nucleic Acid Chemistry
- Peptidase Inhibition and Analysis
- Biofuel production and bioconversion
- Microtubule and mitosis dynamics
- Tree Root and Stability Studies
- Plant responses to water stress
- Plant tissue culture and regeneration
- Porphyrin Metabolism and Disorders
- Light effects on plants
- Plant Parasitism and Resistance
- Histone Deacetylase Inhibitors Research
Ghent University
2011-2024
VIB-UGent Center for Plant Systems Biology
2011-2023
Umeå Plant Science Centre
2016-2021
Swedish University of Agricultural Sciences
2016-2021
Institute of Science and Technology Austria
2013-2020
Vlaams Instituut voor Biotechnologie
2013-2015
Comenius University Bratislava
2010-2011
Abstract Intercellular distribution of the plant hormone auxin largely depends on polar subcellular plasma membrane PIN-FORMED (PIN) transporters. PIN polarity switches in response to different developmental and environmental signals have been shown redirect fluxes mediating certain responses. phosphorylation at sites by kinases is crucial for function. Here we investigate role during gravitropic response. Loss- gain-of-function mutants PINOID related but not D6PK kinase as well mutations...
Significance Polyphosphoinositides (PPIs) are derivatives of the membrane lipid phosphatidylinositol that occur in minor amounts eukaryotic membranes. PPIs have regulatory effects on various cellular processes, but their roles plants currently not well-understood. Plant growth relies largely turgor-driven cell expansion, which at subcellular level is linked to vacuolar dynamics. We identified an unknown subgroup tonoplast-associated enzymes from Arabidopsis thaliana , suppressor actin 2...
The plant hormone auxin is a key regulator of growth and development. Auxin levels are sensed interpreted by distinct receptor systems that activate broad range cellular responses. Auxin-Binding Protein1 (ABP1) has been identified based on its ability to bind with high affinity prime candidate for the extracellular responsible mediating effects, in particular, fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance ABP1 many developmental processes....
Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into is lacking. Here, we reveal molecular architecture of plant [TPLATE (TPC)] using integrative approach. We identify crucial roles for specific subunits in assembly interaction. Our data therefore generate fresh...
Identifying protein-protein interactions (PPIs) is crucial for understanding biological processes. Many PPI tools are available, yet only some function within the context of a plant cell. Narrowing down even further, few allow complex multi-protein to be visualized. Here, we present conditional in vivo tool research that meets these criteria. Knocksideways plants (KSP) based on ability rapamycin alter localization bait protein and its interactors via heterodimerization FKBP FRB domains. KSP...
Abstract Lateral roots are typically maintained at non-vertical angles with respect to gravity. These gravitropic setpoint intriguing because their maintenance requires that able effect growth response both and against the gravity vector, a phenomenon previously attributed gravitropism acting an antigravitropic offset mechanism. Here we show how components mediating in vertical primary root—PINs phosphatases upon them—are reconfigured regulation such lateral root range of can be maintained....
Redirection of intercellular auxin fluxes via relocalization the PIN-FORMED 3 (PIN3) and PIN7 efflux carriers has been suggested to be necessary for root gravitropic response. Cytokinins have also proposed play a role in controlling gravitropism, but conclusive evidence is lacking. We present detailed study dynamics bending early after gravistimulation, which revealed delayed response transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) cytokinin signaling mutants....
Significance As the outermost cell layer of an organism, epidermis plays a key role in controlling morphogenesis. In this work, we investigated cell-shape regulation young, lobing pavement cells Arabidopsis leaf epidermis. By taking advantage their developmental synchrony, showed that establishment local auxin gradient is necessary for initiation first-lobe formation. However, not stable over time but rather fluctuates according to particular stage cells. These changes are established by...
Significance Endocytosis controls the proteome and lipidome of plasma membrane therefore communication between cell outside world. Because its importance, many attempts have been made to develop tools interfere with this process. These include genetic approaches small chemicals. Thus far, however, developed modulate plant endocytosis either lack specificity, are irreversible, or require several days produce an effect. Here, we generated a conditional reversible tool specifically endocytosis....
In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. this work, the introduction fluorophore an auxin molecule represents sensitive and non-invasive method directly visualise localisation with high spatiotemporal resolution. The state-of-the-art multidisciplinary approaches genetic chemical biology analysis together live cell imaging, liquid chromatography-mass spectrometry (LC-MS)...
Cell and tissue polarization is fundamental for plant growth morphogenesis. The polar, cellular localization of Arabidopsis PIN-FORMED (PIN) proteins crucial their function in directional auxin transport. clustering PIN polar cargoes within the plasma membrane has been proposed to be important maintenance distribution. However, more detailed features clusters requirements cargo remain unclear. Here, we characterized detail by means multiple advanced microscopy quantification methods, such as...
Summary Clathrin-mediated endocytosis (CME) is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form membrane-bound vesicles. In plants, evolutionarily ancient TSET/TPLATE complex (TPC) plays essential, but not well-defined role in CME. Here, we show that two highly disordered TPC subunits, AtEH1 AtEH2 function as scaffolds drive biomolecular condensation complex. These condensates specifically nucleate on plasma membrane...
Summary distribution of auxin within plant tissues is great importance for developmental plasticity, including root gravitropic growth. Auxin flow directed by the subcellular polar and dynamic relocalisation transporters such as PIN ‐ FORMED ( ) efflux carriers, which can be influenced main natural indole‐3‐acetic acid IAA ). Anthranilic AA an important early precursor previously published studies with analogues have suggested that may also regulate localisation. Using Arabidopsis thaliana a...
The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, play essential roles endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the localization PIN-FORMED transporters hormone auxin. Here, using chemical genetics screen Arabidopsis thaliana,...