A5030700903- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Advanced Cellulose Research Studies
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Lignin and Wood Chemistry
- Tree Root and Stability Studies
- Wood Treatment and Properties
- Plant responses to water stress
- Forest ecology and management
- Bioenergy crop production and management
- Plant Water Relations and Carbon Dynamics
- Chromosomal and Genetic Variations
- Polysaccharides Composition and Applications
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Aluminum toxicity and tolerance in plants and animals
- Postharvest Quality and Shelf Life Management
- Plant Pathogens and Resistance
- Photosynthetic Processes and Mechanisms
- Food composition and properties
- Plant tissue culture and regeneration
Swedish University of Agricultural Sciences
2015-2025
Umeå Plant Science Centre
2014-2024
Umeå University
2007-2016
KTH Royal Institute of Technology
2004-2010
AlbaNova
2005-2010
Lund University
2010
University of Wrocław
2009
Petersburg Nuclear Physics Institute
2009
Hertfordshire Community NHS Trust
2008
AstraZeneca (Sweden)
2007
Metabolomics studies generate increasingly complex data tables, which are hard to summarize and visualize without appropriate tools. The use of chemometrics tools, e.g., principal component analysis (PCA), partial least-squares latent structures (PLS), orthogonal PLS (OPLS), is therefore great importance as these include efficient, validated, robust methods for modeling information-rich chemical biological data. Here the S-plot proposed a tool visualization interpretation multivariate...
Abstract Plant growth is the result of cell proliferation in meristems, which requires a careful balance between formation new tissue and maintenance set undifferentiated stem cells. Recent studies have provided important information on several genetic networks responsible for regulation differentiation apical meristems shoots roots. Nothing, however, known about regulatory secondary like vascular cambium trees. We made use large size highly regular layered organization cambial meristem to...
Summary Stems and branches of angiosperm trees form tension wood (TW) when exposed to a gravitational stimulus. One the main characteristics TW, which distinguishes it from normal wood, is formation fibers with thick inner gelatinous cell wall layer mainly composed crystalline cellulose. Hence TW enriched in cellulose, deficient lignin hemicelluloses. An expressed sequence tag library made TW‐forming tissues Populus tremula (L.) × tremuloides (Michx.) data transcript profiling using...
Trees represent the largest terrestrial carbon sink and a renewable source of ligno-cellulose. There is significant scope for yield quality improvement in these largely undomesticated species, efforts to engineer elite varieties will benefit from improved understanding transcriptional network underlying cambial growth wood formation. We generated high-spatial-resolution RNA sequencing data spanning secondary phloem, vascular cambium, wood-forming tissues Populus tremula The transcriptome...
Abstract The vascular cambium produces secondary xylem and phloem in plants is responsible for wood formation forest trees. In this study we used a microscale mass-spectrometry technique coupled with cryosectioning to visualize the radial concentration gradient of endogenous indole-3-acetic acid (IAA) across cambial meristem differentiating derivatives Scots pine (Pinus sylvestris L.) trees that had different rates growth. This approach allowed us investigate relationship between growth rate...
Abstract Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, expansins. Poplar (Populus spp.) had approximately 1.6 times more CAZyme than Arabidopsis (Arabidopsis thaliana). Whereas most proportionally increased, xylan pectin-related underrepresented GT1...
Xyloglucan transglycosylases (XETs) have been implicated in many aspects of cell wall biosynthesis, but their function vascular tissues, general, and the formation secondary walls, particular, is less well understood. Using an situ XET activity assay poplar stems, we demonstrated xylem phloem fibers at stage formation. Immunolocalization fucosylated xylogucan with CCRC-M1 antibodies showed that levels this species increased border between primary layers time deposition. Furthermore, one most...
Abstract Indole acetic acid (auxin) is a key regulator of wood formation, and an observed overlap between auxin concentration gradient developing secondary xylem cells has led to the hypothesis that regulates formation by acting as morphogen. We dissected role in identifying auxin-responsive transcriptome wood-forming tissues investigating alterations transgenic hybrid aspen plants (Populus tremula × Populus tremuloides) with perturbed signaling. showed genes respond dynamically changes...
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis currently relatively well understood, the pathways leading to synthesis of key structural carbohydrates in wood fibers remain obscure. We have used functional genomics approach identify enzymes involved carbohydrate and remodeling during xylem development hybrid aspen Populus tremula x tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were...
Abstract Aerenchyma tissues form gas-conducting tubes that provide roots with oxygen under hypoxic conditions. Although aerenchyma have received considerable attention in Zea mays, the signaling events and genes controlling induction remain elusive. Here, we show Arabidopsis thaliana hypocotyls lysigenous response to hypoxia this process involves H2O2 ethylene signaling. By studying mutants are deregulated for excess light acclimation, cell death, defense responses, find formation of depends...
Non-cellulosic cell wall polysaccharides constitute approximately one quarter of usable biomass for human exploitation. In contrast to cellulose, these components are usually substituted by O-acetyl groups, which affect their properties and interactions with other polymers, thus affecting solubility extractability. However, details still largely obscure. Moreover, polysaccharide hydrolysis constituent monosaccharides, is hampered the presence necessitating either enzymatic (esterase) or...
Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position space. fibers develop on one side the stem have an increased potency shrink compared with opposite side, thus creating moment. It believed gelatinous (G) cell wall layer containing almost pure cellulose tension pivotal shrinking. By analyzing saccharide composition and linkage isolated G-layers poplar, we found they contain some matrix components addition cellulose, which...
The ability of bacterial or fungal necrotrophs to produce enzymes capable degrading pectin is often related a successful initiation the infective process. Pectin synthesized in highly methylesterified form and subsequently de-esterified muro by methylesterase. De-esterification makes more susceptible degradation pectic such as endopolygalacturonases (endoPG) pectate lyases secreted necrotrophic pathogens during first stages infection. We show that, upon infection, Pectobacterium carotovorum...
Plant fibers are one of the most important renewable resources, used as raw material in paper industry, and for various textiles composites. Fibers structural components timber an energy-rich component fuel-wood. For plant itself, establishing architecture, a source mechanical support, defence from herbivory, some cases elements with contractile properties, resembling those muscles. In addition, may store ergastic carbon resources water. Here, we review aspects fiber development such...
Growth and biomechanics of etiolated hypocotyls from Arabidopsis thaliana lines overexpressing xyloglucan endotransglucosylase/hydrolase AtXTH18, AtXTH19, AtXTH20, PttXET16-34 were studied. Overexpression AtXTH20 stimulated growth hypocotyls, while overexpression did not show this effect. In vitro extension frozen/thawed measured by a constant-load extensiometer started high-amplitude initial deformation followed slow time-dependent creep. Creep growing XTH-overexpressing (OE) was more...
Wood cells, unlike most other cells in plants, grow by a unique combination of intrusive and symplastic growth. Fibers diameter diffuse growth, but they elongate solely apical growth penetrating the pectin-rich middle lamella that cements neighboring together. In contrast, vessel elements We demonstrate an abundant pectin methyl esterase (PME; EC 3.1.1.11) from wood-forming tissues hybrid aspen (Populus tremula x tremuloides) acts as negative regulator both developing wood cells. When...
Tension wood evolved in woody angiosperms to allow stems with secondary thickening bend and thus maintain an optimal orientation. Stem bending is the result of longitudinal tensile stress that develops tension tissues. In many species, a specialized cell wall layer, so-called gelatinous (G)-layer, develops, containing longitudinally orientated crystalline cellulose fibrils; these have been recently shown generate by unknown mechanism. The fibrils cannot, however, work isolation. Both...
Differentiation of xylem cells in dicotyledonous plants involves expansion the radial primary cell walls and intrusive tip growth cambial derivative prior to deposition a thick secondary wall essential for function. Expansins are wall-residing proteins that have an ability plasticize cellulose-hemicellulose network walls. We found expansin activity extracted from region mature stems model tree species hybrid aspen (Populus tremula x Populus tremuloides Michx). identified three alpha-expansin...