A5027895791- Cocoa and Sweet Potato Agronomy
- Food Chemistry and Fat Analysis
- Studies on Chitinases and Chitosanases
- Phytoplasmas and Hemiptera pathogens
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Phytochemical compounds biological activities
- Banana Cultivation and Research
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Sugarcane Cultivation and Processing
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Growth and nutrition in plants
- Plant nutrient uptake and metabolism
- Plant Pathogens and Resistance
- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Species Descriptions
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2016-2025
Genetic Improvement and Adaptation of Mediterranean and Tropical Plants
2016-2025
Universidade Estadual de Santa Cruz
2016-2025
Agropolis International
2007-2020
Comissão Executiva do Plano da Lavoura Cacaueira
2017
UMR QualiSud
2017
Inesc P&D Brasil
2003-2010
Département Biologie et Amélioration des Plantes
2009
Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic
2009
GTx (United States)
2008
Witches' broom disease (WBD) of cacao (Theobroma L.), caused by Moniliophthora perniciosa, is the most important limiting factor for production in Brazil. Hence, development genotypes with durable resistance key challenge control disease. Proteomic methods are often used to study interactions between hosts and pathogens, therefore helping classical plant breeding projects on resistant genotypes. The present compared proteomic alterations two standard WBD susceptibility, response M....
The present study evaluated the physiological, molecular and hormonal parameters from scion/rootstock interaction of citrus plants during recurrent water deficit. Responses Valencia (VO) scion variety grafted on two rootstocks with different soil extraction capacities, Rangpur Lime (RL) Sunki Maravilha (SM), three successive periods deficit: exposed to a single episode deficit (WD1) (WD2) (WD3) WD were compared. combinations VO/RL VO/SM presented polymorphic alterations epigenetic marks...
We report on a large‐scale expressed sequence tag (EST) sequencing and analysis program aimed at characterizing the sets of genes in roots model legume Medicago truncatula during interactions with either two microsymbionts, nitrogen‐fixing bacterium Sinorhizobium meliloti or arbuscular mycorrhizal fungus Glomus intraradices. have designed specific tools for silico EST data, relation to chimeric cDNA detection, clustering, encoded protein prediction, detection differential expression. Our 21...
Wood cells, unlike most other cells in plants, grow by a unique combination of intrusive and symplastic growth. Fibers diameter diffuse growth, but they elongate solely apical growth penetrating the pectin-rich middle lamella that cements neighboring together. In contrast, vessel elements We demonstrate an abundant pectin methyl esterase (PME; EC 3.1.1.11) from wood-forming tissues hybrid aspen (Populus tremula x tremuloides) acts as negative regulator both developing wood cells. When...
Preparation of high-quality proteins from cacao vegetative organs is difficult due to very high endogenous levels polysaccharides and polyphenols. In order establish a routine procedure for the application proteomic biochemical analysis tissues, three new protocols were developed; one apoplastic washing fluid (AWF) extraction, two protein extraction--under denaturing nondenaturing conditions. The first described method allows quick easy collection AWF--using infiltration-centrifugation...
Witches' broom disease is caused by the hemibiotrophic basidiomycete Moniliophthora perniciosa, and one of most important diseases cacao in western hemisphere. Because very little known about global process such development, expressed sequence tags (ESTs) were used to identify genes during Theobroma cacao-Moniliophthora perniciosa interaction.Two cDNA libraries corresponding resistant (RT) susceptible (SP) cacao-M. interactions constructed from total RNA, using DB SMART Creator library kit...
The hemibiotrophic pathogens Moniliophthora perniciosa (witches' broom disease) and roreri (frosty pod rot are among the most important of cacao. has a broad host range infects variety meristematic tissues in cacao plants, whereas M. only pods Theobroma Herrania genera. Comparative pathogenomics these fungi is essential to understand infection strategies, therefore detection silico functional characterization effector candidates steps gain insight on their pathogenicity.Candidate secreted...
The production and accumulation of pathogenesis-related proteins (PR proteins) in plants response to biotic or abiotic stresses is well known considered as a crucial mechanism for plant defense. A protein 4 cDNA was identified from cacao-Moniliophthora perniciosa interaction library named TcPR-4b. TcPR-4b presents Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling confirmed importance residues maintain structure, several amino acids...
Abstract Biochemical microanalysis combined with tangential cryosectioning was used to visualize the distribution of pectin methylesterases (PMEs) across cambial region in active and dormant hybrid aspen (Populus tremula L. × Populus tremuloides Michx). These novel techniques allowed us relate activity isoforms PMEs specific tissues developmental stages stem get more information on physiological function growth. Isoelectrofocusing analysis revealed numerous that were differentially...
Scion/rootstock interaction is important for plant development and breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic abiotic stresses. Here we analyzed the response drought of two different scion/rootstock combinations presenting polyploidy: diploid (2x) autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) grafted with 2x Valencia Delta sweet orange sinensis) scions, named V/2xRL V/4xRL, respectively. Based on previous...
Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used organism to enhance or repress gene expression in response stimuli. Here, we report on genome-wide analysis Theobroma cacao TF family and also investigate genes infected fungus Moniliophthora perniciosa. In genome, 61 non-redundant sequences were found classified three groups (I III) according zinc-finger motif types. The putative distributed 10 chromosomes 24 them came from...
The cytogenomic study of repetitive regions is fundamental for the understanding morphofunctional mechanisms and genome evolution. Passiflora edulis a species relevant agronomic value, this work had its sequenced by next generation sequencing bioinformatics analysis performed RepeatExplorer pipeline. clusters allowed identification characterization elements (predominant contributors to most plant genomes). aim was identify, characterize map DNA P. edulis, providing important markers,...
ABSTRACT Most of the rich genetic diversity observed in cultivated citrus results from a reticulate evolution involving four ancestral taxa, C. medica , reticulata maxima and micrantha whose radiation occurred allopatry. In such an evolutive context, genome studies, GWAS analysis transcriptomic studies will be significantly enhanced through pangenome approaches. We report implementation super-pangenome for citrus, established with de novo assemblies pseudochromosomes (346 Mb), (332 Mb) (333...
Detecting pathogens in asymptomatic plants allows for a rapid response to emerging threats, such as the introduction of new or emergence resistant strains. Frosty pod rot ( Moniliophthora roreri) significantly jeopardizes cacao production by impairing quality and yield. However, early diagnosis is challenging due its prolonged incubation period, compounded symptoms resembling those M. perniciosa, causative agent witches’ broom disease. To address this, specific sensitive quantitative...
We set up a large-scale suppression subtractive hybridization (SSH) approach to identify Medicago truncatula genes differentially expressed at different stages of the symbiotic interaction with Sinorhizobium meliloti, particular interest for regulatory genes. constructed 7 SSH libraries covering successive from Nod factor signal transduction S. meliloti infection, nodule organogenesis, and functioning. Over 26,000 clones were screened by two rounds macroarray hybridizations. In all, 3,340...