A5083621323- Mitochondrial Function and Pathology
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Plant Reproductive Biology
- Cellular transport and secretion
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Neuroscience and Neuropharmacology Research
- Photosynthetic Processes and Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Polysaccharides and Plant Cell Walls
- ATP Synthase and ATPases Research
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Advanced Proteomics Techniques and Applications
- Retinal Development and Disorders
- Machine Learning in Bioinformatics
University of Gothenburg
2019-2024
Sahlgrenska University Hospital
2020-2024
Stanford University
2016-2020
Howard Hughes Medical Institute
2020
Karolinska Institutet
2008-2012
Max Planck Society
2011
Umeå University
1998-2006
Umeå Plant Science Centre
1998-2006
Oak Ridge National Laboratory
2006
University of Tennessee at Knoxville
2006
We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...
Antibody-based proteomics provides a powerful approach for the functional study of human proteome involving systematic generation protein-specific affinity reagents. We used this strategy to construct comprehensive, antibody-based protein atlas expression and localization profiles in 48 normal tissues 20 different cancers. Here we report new publicly available database containing, first version, ∼400,000 high resolution images corresponding more than 700 antibodies toward proteins. Each...
A rapidly growing area of genome research is the generation expressed sequence tags (ESTs) in which large numbers randomly selected cDNA clones are partially sequenced. The collection ESTs reflects level and complexity gene expression sampled tissue. To date, majority plant from nonwoody plants such as Arabidopsis , Brassica maize, rice. Here, we present a large-scale production wood-forming tissues two poplars, Populus tremula L. × tremuloides Michx. trichocarpa ‘Trichobel.’ 5,692 analyzed...
Mitochondrial dysfunction is heavily implicated in Parkinson disease (PD) as exemplified by the finding of an increased frequency respiratory chain-deficient dopamine (DA) neurons affected patients. An inherited form PD caused impaired function Parkin, E3 ubiquitin ligase reported to translocate defective mitochondria vitro facilitate their clearance. We have developed a reporter mouse assess mitochondrial morphology DA vivo and show here that chain deficiency leads fragmentation network...
Tauopathies are age-associated neurodegenerative diseases whose mechanistic underpinnings remain elusive, partially due to a lack of appropriate human models. Here, we engineered induced pluripotent stem cell (hiPSC)-derived neuronal lines express 4R Tau and carrying the P301S MAPT mutation when differentiated into neurons. 4R-P301S neurons display progressive inclusions upon seeding with fibrils recapitulate features tauopathy phenotypes including shared transcriptomic signatures,...
Trees present a life form of paramount importance for terrestrial ecosystems and human societies because their ecological structure physiological function provision energy industrial materials. The genus Populus is the internationally accepted model molecular tree biology. We have analyzed 102,019 ESTs that clustered into 11,885 clusters 12,759 singletons. also provide >4,000 assembled full clone sequences to serve as basis upcoming annotation genome sequence. A public web-based EST database...
Abstract Two cDNA libraries were prepared, one from leaves of a field-grown aspen (Populus tremula) tree, harvested just before any visible sign leaf senescence in the autumn, and young but fully expanded greenhouse-grown tremula × tremuloides). Expressed sequence tags (ESTs; 5,128 4,841, respectively) obtained two libraries. A semiautomatic method annotation functional classification ESTs, according to modified Munich Institute Protein Sequences scheme, was developed, utilizing information...
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis currently relatively well understood, the pathways leading to synthesis of key structural carbohydrates in wood fibers remain obscure. We have used functional genomics approach identify enzymes involved carbohydrate and remodeling during xylem development hybrid aspen Populus tremula x tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were...
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1), specific to neurons and muscle, supplies aspartate the cytosol and, as a component of malate-aspartate shuttle, enables oxidation cytosolic NADH, thought be important in providing energy for central nervous system. We describe AGC1 deficiency, novel syndrome characterized by arrested psychomotor development, hypotonia, seizures child with homozygous missense mutation solute family 25, member 12, gene SLC25A12, which encodes...
Mitochondrial dysfunction is implicated in aging and degenerative disorders such as Parkinson's disease (PD). Continuous fission fusion of mitochondria shapes their morphology essential to maintain oxidative phosphorylation. Loss-of-function mutations PTEN-induced kinase1 (PINK1) or Parkin cause a recessive form PD have been linked altered regulation mitochondrial dynamics. More specifically, the E3 ubiquitin ligase has shown directly regulate levels mitofusin 1 (Mfn1) Mfn2, two homologous...
Three neuronal pentraxins are expressed in brain, the membrane-bound “neuronal pentraxin receptor” (NPR) and secreted proteins NP1 NARP (i.e., NP2). Neuronal bind to AMPARs at excitatory synapses play important, well-documented roles activity-dependent regulation of neural circuits via this binding activity. However, it is unknown whether perform beyond modulating postsynaptic AMPAR-dependent plasticity, they may even act inhibitory synapses. Here, we show that NPR non-neuronal cells...
Significance Interactions between transsynaptic cell-adhesion molecules enable formation of trillions synaptic connections that wire neurons into vast functional circuits. Neurexins are presynaptic bind to diverse postsynaptic ligands, and genetically linked neuropsychiatric disorders. Here we show CA10, a secreted, catalytically inactive carbonic-anhydrase homolog, binds in cis configuration all neurexins, including Nrxn1γ. In cultured neurons, CA10 enhances surface transport indicating the...
ABSTRACT The formation of mammalian synapses entails the precise alignment presynaptic release sites with postsynaptic receptors but how nascent cell–cell contacts translate into assembly specializations remains unclear. Guided by pioneering work in invertebrates, we hypothesized that synapses, liprin-α proteins directly link trans -synaptic initial to downstream steps. Here show that, human neurons lacking all four isoforms, synaptic are formed recruitment active zone components and...
Summary We analysed the publicly available expressed sequence tag (EST) collections for genus Populus to examine whether evidence can be found large‐scale gene‐duplication events in evolutionary past of this genus. The ESTs were clustered into unigenes each poplar species examined. Gene families constructed all proteins deduced from these unigenes, and K S dating was performed on paralogs within a gene family. fraction then plotted against values, which resulted distribution reflecting age...
Differentiation of xylem cells in dicotyledonous plants involves expansion the radial primary cell walls and intrusive tip growth cambial derivative prior to deposition a thick secondary wall essential for function. Expansins are wall-residing proteins that have an ability plasticize cellulose-hemicellulose network walls. We found expansin activity extracted from region mature stems model tree species hybrid aspen (Populus tremula x Populus tremuloides Michx). identified three alpha-expansin...
A variety of observations support the hypothesis that deficiency complex I [reduced nicotinamide-adenine dinucleotide (NADH):ubiquinone oxidoreductase] mitochondrial respiratory chain plays a role in pathophysiology Parkinson's disease (PD). However, recent data from study using mice with knockout subunit NADH:ubiquinone oxidoreductase iron-sulfur protein 4 (Ndufs4) has challenged this concept as these show degeneration non-dopamine neurons. In addition, primary dopamine (DA) neurons derived...
Protein functional analysis in the post-genomic era is a huge task that has to be approached by different methods parallel. The use of protein-specific antibodies conjunction with tissue microarrays proven one important technology. In this study, we present strategy for optimized design protein subfragments subsequent antibody production. fragments are selected based on principle lowest sequence similarity other human proteins, optimally generate high selectivity. Furthermore, should have...