A5003353061- Cellular transport and secretion
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Hippo pathway signaling and YAP/TAZ
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Biochemical and Molecular Research
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Wnt/β-catenin signaling in development and cancer
- Muscle Physiology and Disorders
- RNA modifications and cancer
- Synthetic Organic Chemistry Methods
- Nuclear Structure and Function
- Hormonal and reproductive studies
- DNA and Nucleic Acid Chemistry
- Ion channel regulation and function
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
Southern University of Science and Technology
2016-2025
Zhengzhou University
2024-2025
Nankai University
2025
Nanjing University
2025
Shenzhen University
2024
Sichuan Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2024
Shenyang University of Technology
2024
University of Gothenburg
2023
Heidelberg University
2023
Kindlins and talins are integrin-binding proteins that critically involved in integrin activation, an essential process for many fundamental cellular activities including cell-matrix adhesion, migration, proliferation. As FERM-domain-containing proteins, kindlins, respectively, bind different regions of β-integrin cytoplasmic tails. However, compared with the extensively studied talin, little is known about how kindlins specifically interact integrins synergistically enhance their activation...
The tumor suppressor Merlin/NF2 functions upstream of the core Hippo pathway kinases Lats1/2 and Mst1/2, as well nuclear E3 ubiquitin ligase CRL4DCAF1. Numerous mutations Merlin have been identified in Neurofibromatosis type 2 other cancer patients. Despite more than two decades research, regulator remains unknown. Here we show by high-resolution crystal structures that Lats1/2-binding site on FERM domain is physically blocked Merlin's auto-inhibitory tail. Angiomotin binding releases...
Human steroid 5α-reductase 2 (SRD5A2) is an integral membrane enzyme in metabolism and catalyzes the reduction of testosterone to dihydrotestosterone. Mutations SRD5A2 gene have been linked deficiency prostate cancer. Finasteride dutasteride, as inhibitors, are widely used antiandrogen drugs for benign hyperplasia. The molecular mechanisms underlying catalysis inhibition other eukaryotic reductases remain elusive due a lack structural information. Here, we report crystal structure human at...
ABSTRACT The formation of mammalian synapses entails the precise alignment presynaptic release sites with postsynaptic receptors but how nascent cell–cell contacts translate into assembly specializations remains unclear. Guided by pioneering work in invertebrates, we hypothesized that synapses, liprin-α proteins directly link trans -synaptic initial to downstream steps. Here show that, human neurons lacking all four isoforms, synaptic are formed recruitment active zone components and...
Glycosylation chemistry plays a pivotal role in glycoscience. Recent substantial developments have poised the field to address emerging challenges related sustainability, cost efficiency, and robust applicability complex substrate settings. The transition from stoichiometric activation metal-catalyzed methods promises enhanced chemoselectivity greater precision controlling glycosidic bond breakage formation, key overcoming existing obstacles. Here, we report nitrene-mediated glycosylation...
Ni-dependent carbon monoxide dehydrogenases (Ni-CODHs) are a diverse family of enzymes that catalyze reversible CO:CO(2) oxidoreductase activity in acetogens, methanogens, and some CO-using bacteria. Crystallography Ni-CODHs from bacteria acetogens has revealed the overall fold Ni-CODH core suggested structures for C cluster mediates interconversion. Despite these advances, mechanism CO oxidation remained elusive. Herein, we report structure distinct class methanogenic archaea:...
The unconventional myosin VIIa (MYO7A) is one of the five proteins that form a network complexes involved in formation stereocilia. Defects these cause syndromic deaf-blindness humans [Usher syndrome I (USH1)]. Many disease-causing mutations occur tail homology 4-protein 4.1, ezrin, radixin, moesin (MyTH4-FERM) domains binds to another USH1 protein, Sans. We report crystal structure MYO7A MyTH4-FERM complex with central domain (CEN) Sans at 2.8 angstrom resolution. MyTH4 and FERM an integral...
Significance The Crumbs–PALS1–PATJ complex is vital for the development and maintenance of polarity diverse tissues. Defects in formation are known to cause diseases such as cancer blindness. highly conserved cytoplasmic tail transmembrane protein Crumbs responsible apical targeting complex. However, molecular basis governing specific Crumbs–PALS1 poorly understood. We discovered that PDZ–SH3–GK tandem PALS1 forms a structural supramodule interacting with large part high affinity...
Ankyrin adaptors together with their spectrin partners coordinate diverse ion channels and cell adhesion molecules within plasma membrane domains thereby promote physiological activities including fast signaling in the heart nervous system. Ankyrins specifically bind to numerous targets through 24 ankyrin repeats (ANK repeats), although mechanism for facile independent evolution of these interactions has not been resolved. Here we report structures ANK complex an inhibitory segment from...
Myosin X (MyoX), encoded by Myo10 , is a representative member of the MyTH4–FERM domain-containing myosins, and this family unconventional myosins shares common functions in promoting formation filopodia/stereocilia structures many cell types with unknown mechanisms. Here, we present structure MyoX tandem complex cytoplasmic tail P3 domain netrin receptor DCC. The structure, together biochemical studies, reveals that MyTH4 FERM domains interact each other, forming structural functional...
Processive movements of unconventional myosins on actin filaments generally require motor dimerization. A commonly accepted myosin dimerization mechanism is via formation a parallel coiled-coil dimer by stretch amino acid residues immediately carboxyl-terminal to the motor's lever-arm domain. Here, we discover that predicted region X forms highly stable, antiparallel (anti-CC). Disruption anti-CC either single-point mutations or replacement with coiled coil similar length compromised...
Background— The cardiac cytoskeleton plays key roles in maintaining myocyte structural integrity health and disease. In fact, human mutations cytoskeletal elements are tightly linked to pathologies, including myopathies, aortopathies, dystrophies. Conversely, the link between protein dysfunction electric activity is not well understood often overlooked arrhythmia field. Methods Results— Here, we uncover a new mechanism for regulation of membrane excitability. We report that βII spectrin, an...
Synaptic scaffold proteins (e.g., liprin-α, ELKS, RIM, and RIM-BP) orchestrate ion channels, receptors, enzymes at presynaptic terminals to form active zones for neurotransmitter release. The underlying mechanism of the zone assembly remains elusive. Here, we report that liprin-α have potential oligomerize through N-terminal coiled-coil region. Our structural biochemical characterizations reveal a gain-of-function mutation promotes self-assembly coiled coils in liprin-α2 by disrupting...
Abstract Myosin VI is the only molecular motor that moves towards minus end along actin filaments. Numerous cellular processes require myosin and tight regulations of motor’s activity. Defects in activity are known to cause genetic diseases such as deafness cardiomyopathy. However, mechanisms underlying regulation remain elusive. Here, we determined high-resolution cryo-electron microscopic structure its autoinhibited state. Our reveals adopts a compact, monomeric conformation via extensive...