A5059652371- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Supramolecular Self-Assembly in Materials
- Virus-based gene therapy research
- Cancer-related gene regulation
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Algal biology and biofuel production
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Gold and Silver Nanoparticles Synthesis and Applications
- Signaling Pathways in Disease
- Neurogenetic and Muscular Disorders Research
- DNA and Nucleic Acid Chemistry
- Trace Elements in Health
- Data Visualization and Analytics
- Chromosomal and Genetic Variations
- Nanoparticle-Based Drug Delivery
- Biopolymer Synthesis and Applications
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Chromatin Remodeling and Cancer
State Key Laboratory of Medicinal Chemical Biology
2013-2024
Nankai University
2014-2024
Second Affiliated Hospital of Chengdu University of Traditional Chinese
2024
Central South University
2015-2024
Southern University of Science and Technology
2024
Huazhong University of Science and Technology
2019-2023
Nanjing University of Chinese Medicine
2022
South China Agricultural University
2022
Jinan Central Hospital
2022
Shandong First Medical University
2022
Magnesium (Mg(2+)) is the second most abundant cation in cells, yet relatively few mechanisms have been identified that regulate cellular levels of this ion. The clearly Mg(2+) transporters are bacteria and yeast. Here, we use a yeast complementary screen to identify two mammalian genes, MagT1 TUSC3, as major influx. universally expressed all human tissues its expression level up-regulated low extracellular Mg(2+). Knockdown either or TUSC3 protein significantly lowers total free...
To transform common low-molecular-weight (LMW) cationic polymers, such as polyethylenimine (PEI), to highly efficient gene vectors would be of great significance but remains challenging. Because LMW polymers perform far less efficiently than their high-molecular-weight counterparts, mainly due weaker nucleic acid encapsulation, herein we report the design and synthesis a dipicolylamine-based disulfide-containing zinc(II) coordinative module (Zn-DDAC), which is used functionalize PEI (Mw ≈...
The tumor suppressor Merlin/NF2 functions upstream of the core Hippo pathway kinases Lats1/2 and Mst1/2, as well nuclear E3 ubiquitin ligase CRL4DCAF1. Numerous mutations Merlin have been identified in Neurofibromatosis type 2 other cancer patients. Despite more than two decades research, regulator remains unknown. Here we show by high-resolution crystal structures that Lats1/2-binding site on FERM domain is physically blocked Merlin's auto-inhibitory tail. Angiomotin binding releases...
Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design smart plasmonic nanobiosensor based on individual Au@Ag core–shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA) for detecting microRNA 21 (miR-21) level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift approximately 0.4 nm can be obtained single miR-21...
MAPK4 is an atypical MAPK. Currently, little known about its physiological function and involvement in diseases, including cancer. A comprehensive analysis of 8887 gene expression profiles The Cancer Genome Atlas (TCGA) revealed that overexpression correlates with decreased overall survival, particularly marked survival effects patients lung adenocarcinoma, bladder cancer, low-grade glioma, thyroid carcinoma. Interestingly, human tumor also correlated phosphorylation AKT, 4E-BP1, p70S6K,...
Self-sorting is a common phenomenon in eukaryotic cells and represents one of the versatile strategies for formation advanced functional materials; however, developing artificial self-sorting assemblies within living remains challenging. Here, we report on GSH-responsive situ peptide cancer simultaneous organelle targeting to promote combinatorial dysfunction thereby cell death. The system was created via design two peptides E3C16E6 EVMSeO derived from lipid-inspired interdigitating...
Enzyme-regulated in situ self-assembly of peptides represents one versatile strategy the creation theranostic agents, which, however, is limited by strong dependence on enzyme overexpression. Herein, we reported self-amplifying assembly precisely macrophages associated with expression for improving anti-inflammatory efficacy conventional drugs. The assembling system was created via coassembling an enzyme-responsive peptide its derivative functionalized a protein ligand. Reduction NAD(P)H...
From insects to mammals, metallothionein genes are induced in response heavy metal load by the transcription factor MTF-1, which binds short DNA sequence motifs, termed elements (MREs). Here we describe a novel and seemingly paradoxical role for MTF-1 Drosophila that it also mediates transcriptional activation of Ctr1B, copper importer, upon depletion. Activation depends on same type MRE motifs upstream region Ctr1B gene as normally required induction. Thus, single factor, plays direct both...
Responsive hydrogels hold great potential in controllable drug delivery, regenerative medicine, sensing, etc. We introduced this study the first example of a photo-responsive protein for hydrogel formation. Based on crystal structure protein, Arabidopsis thaliana UVR8, we designed and expressed its derived UVR8-1 with hexa-peptide WRESAI. also prepared supramolecular nanofibers TIP-1 at their surface. The simple mixing these two components resulted rapid formation through specific...
Mitochondrion-targeting therapy exhibits great potential in cancer but significantly suffers from limited therapeutic efficiency. Here we report on mitochondrion-targeting supramolecular antagonist-inducing tumor cell death via simultaneously promoting cellular apoptosis and preventing survival. The antagonist was created coassembly of a pentapeptide with its two derivatives functionalized BH3 domain or the drug camptothecin (CPT). While CPT released induced decreasing mitochondrial membrane...
Abstract Oncoprotein SS18-SSX is a hallmark of synovial sarcomas. However, as part the fusion protein, SS18’s function remains unclear. Here, we depict structures both human SS18/BRG1 and yeast SNF11/SNF2 subcomplexes. Both subcomplexes assemble into heterodimers that share similar conformation, suggesting SNF11 might be homologue SS18 in chromatin remodeling complexes. Importantly, our study shows self-association intrinsically disordered region, QPGY domain, leads to liquid-liquid phase...
The trace element copper is required for normal growth and development, serving as an essential catalytic co-factor enzymes involved in energy generation, oxidative stress protection, neuropeptide maturation, other fundamental processes. In yeast mammals acquisition occurs through the action of Ctr1 family high affinity transporters. Here we describe studies using Drosophila melanogaster to investigate role development. Three distinct genes (Ctr1A, Ctr1B, Ctr1C) have been identified, which...
Copper is an essential nutrient that serves as a co-factor for enzymes involved in critical cellular processes including energy generation, peptide hormone maturation, oxidative stress protection, and iron homeostasis. Although genes have been identified from yeast mammals encoding homologous subunit of plasma membrane high affinity copper transporter, the presence additional subunits function part transport complex has not reported. We observed ctr4(+), previously protein fission...
Abstract An efficient diastereo‐ and enantioselective [3+2] cycloaddition reaction of α‐aryl isocyanoacetates to isatins catalyzed by a quinine‐derived bifunctional amine‐thiourea‐bearing sulfonamide as multiple hydrogen‐bonding donor catalyst has been investigated. The corresponding adducts, which bear spirocyclic quaternary stereocenter at the C‐3 position oxindole, were obtained in good yields (51–95%), high diastereoselectivities (up >20:1 dr ) excellent enantioselectivities 97% ee ).
Abstract The highly conserved Paf1 complex (PAF1C) plays critical roles in RNA polymerase II transcription elongation and the regulation of histone modifications. It has also been implicated other diverse cellular activities, including posttranscriptional events, embryonic development cell survival maintenance stem identity. Here, we report structure human Paf1/Leo1 subcomplex within PAF1C. overall reveals that Leo1 subunits form a tightly associated heterodimer through antiparallel...
Apoptosis is involved in the pathology of a variety diseases. The measurement apoptosis will help us to evaluate onset disease and effect therapeutic interventions. In addition, increased demand for understanding early stages pushing envelope solutions instance real-time monitoring death kinetics. Here we present novel electrochemiluminescent cytosensing strategy quantitate apoptotic cell numbers, screen some anticancer drugs, their effects on hepatocarcinoma line (HepG2) cells by utilizing...
Significance Elongator is a highly conserved multiprotein complex composed of six subunits elongator protein 1 (Elp1 to -6). has been associated with various cellular activities and attracted clinical attention because its role in certain neurodegenerative diseases. To understand the mechanism assembly, we identified dimerization domain both human yeast Elp1 solved crystal structures domains. This study mechanistic analysis dimerization, which plays an essential integrity functional suggests...