A5053686106- Viral Infections and Vectors
- Cardiomyopathy and Myosin Studies
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- interferon and immune responses
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Viral Infections and Outbreaks Research
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Signaling Pathways in Disease
- Lipid Membrane Structure and Behavior
- Plant Virus Research Studies
- Nuclear Structure and Function
- Peptidase Inhibition and Analysis
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Cell death mechanisms and regulation
- Climate change and permafrost
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Tuberculosis Research and Epidemiology
Southern University of Science and Technology
2020-2025
Shenzhen University
2024
Institute of Biophysics
2013-2016
Chinese Academy of Sciences
2008-2016
ShanghaiTech University
2015
University of Chinese Academy of Sciences
2013
The lysin LysGH15, which is derived from the staphylococcal phage GH15, demonstrates a wide lytic spectrum and strong activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we find that of full-length LysGH15 its CHAP domain dependent on calcium ions. To elucidate molecular mechanism, structures three individual domains were determined. Unexpectedly, crystal structure reveals an "EF-hand-like" calcium-binding site near Cys-His-Glu-Asn quartet active groove. date, in...
Severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel bunyavirus (SFTSV). Lack of vaccines and inadequate therapeutic treatments have made the spread virus global concern. Viral nucleocapsid protein (N) essential for its transcription replication. Here, we present crystal structures N from SFTSV homologs Buenaventura (BUE) Granada (GRA) viruses. The reveal that phleboviral folds into compact core domain extended N-terminal arm mediates...
STAT6 participates in classical IL-4/IL-13 signaling and stimulator of interferon genes-mediated antiviral innate immune responses. Aberrations STAT6-mediated are linked to development asthma diseases the system. In addition, remains constitutively active multiple types cancer. Therefore, targeting is an attractive proposition for treating related diseases. Although a lot known about role transcriptional regulation, molecular details on how recognizes binds specific segments DNA exert its...
Negative-stranded RNA viruses cover their genome with nucleoprotein (N) to protect it from the human innate immune system. Abrogation of function N offers a unique opportunity combat spread viruses. Here, we describe fold Leanyer virus (LEAV, Orthobunyavirus genus, Bunyaviridae family) in complex single-stranded refined 2.78 Å resolution as well 2.68 structure LEAV N–ssDNA complex. is made up an N- and C-terminal lobe, binding site located at junction these lobes. The tetramer binds...
Synaptic scaffold proteins (e.g., liprin-α, ELKS, RIM, and RIM-BP) orchestrate ion channels, receptors, enzymes at presynaptic terminals to form active zones for neurotransmitter release. The underlying mechanism of the zone assembly remains elusive. Here, we report that liprin-α have potential oligomerize through N-terminal coiled-coil region. Our structural biochemical characterizations reveal a gain-of-function mutation promotes self-assembly coiled coils in liprin-α2 by disrupting...
Abstract Myosin VI is the only molecular motor that moves towards minus end along actin filaments. Numerous cellular processes require myosin and tight regulations of motor’s activity. Defects in activity are known to cause genetic diseases such as deafness cardiomyopathy. However, mechanisms underlying regulation remain elusive. Here, we determined high-resolution cryo-electron microscopic structure its autoinhibited state. Our reveals adopts a compact, monomeric conformation via extensive...
As the prototype of unconventional myosin motor family, Va (MyoVa) transport cellular cargos along actin filaments in diverse processes. The off-duty MyoVa adopts a closed and autoinhibited state, which can be relieved by cargo binding. molecular mechanisms governing autoinhibition activation remain unclear. Here, we report cryo-electron microscopy structure two full-length, heavy chains complex with 12 calmodulin light at 4.78-Å resolution. triangular multiple intra- interpolypeptide chain...
A psychrotolerant bacterium, designated strain Tibet-IIU11T, was isolated from the Qinghai--Tibet Plateau permafrost region, China. taxonomic study conducted using a polyphasic approach, with determination of physiological and biochemical properties phylogenetic analysis based on 16S rRNA gene sequences. The novel isolate found to belong genus Hymenobacter distinct recognized species this genus. major fatty acids were iso-C15 : 0 (24.5 %), summed feature 3 (C16 1 ω7c and/or iso C15 2-OH;...
Cellular, biochemical, and structural investigation of the MyoVa/MICAL1 complex reveals an intracellular cargo-unloading mechanism.
<title>Abstract</title> Cell adhesion molecules (CAMs) are pivotal in establishing and maintaining synaptic connectivity. Emerging evidence indicates that some secreted factors within the cleft, including C1q-like proteins (C1qls), play a crucial role bridging pre- post-synapses by connecting bilateral CAMs. However, mechanisms of those synapse assembly remain incomplete. Here, we explored C1ql-mediated connectivity, focusing on C1ql1 its post-synaptic receptor brain-specific angiogenesis...
Uch37 is a de-ubiquitinating enzyme that activated by Rpn13 and involved in the proteasomal degradation of proteins. The full-length was shown to exhibit low iso-peptidase activity thought be auto-inhibited. Structural comparisons revealed within homo-dimer Uch37, each catalytic domains blocking other’s ubiquitin (Ub)-binding site. This blockage likely prevented Ub from entering active site might form basis auto-inhibition. To understand mode auto-inhibition clearly shed light on activation...
SH3 domains are common protein binding modules. The target sequence of is usually a proline-rich motif (PRM) containing minimal "PxxP" sequence. mechanism how different specifically choose their targets from vast PxxP-containing sequences still not very clear, as many reported SH3/PRM interactions weak and promiscuous. Here, we identified the domain ASAP1 to PRM MICAL1 with sub-μM affinity, determined crystal structure ASAP1-SH3 MICAL1-PRM complex. Our structural biochemical analyses...
MICAL proteins represent a unique family of actin regulators crucial for synapse development, membrane trafficking, and cytokinesis. Unlike classical regulators, MICALs catalyze the oxidation specific residues within filaments to induce robust filament disassembly. The potent activity requires tight control prevent extensive damage cytoskeleton. However, molecular mechanism governing MICALs' regulation remains elusive. Here, we report cryo-EM structure MICAL1 in autoinhibited state,...
Myo2, a yeast class V myosin, transports broad range of organelles and plays important roles in various cellular processes, including cell division budding yeast. Despite the fact that several structures Myo2/cargo adaptor complexes have been determined, understanding versatile cargo-binding modes Myo2 is still very limited, given large number cargo adaptors identified for Myo2. Here, we used ColabFold, an AlphaFold2-powered easy-to-use tool, to predict complex Myo2-GTD its adaptors. After...
<title>Abstract</title> MICAL (Molecule Interacting with CasL) proteins represent a unique family of actin regulators crucial for synapse development, membrane trafficking, and cytokinesis. Unlike classical regulators, possess enzymatic activity, catalyzing the oxidation specific residues within filaments to induce robust filament severing. The potent activity requires tight control prevent extensive damage cytoskeleton. Due limited structural information on full-length proteins, molecular...
Abstract PRMT1 is the predominant type I protein arginine methyltransferase responsible for generating monomethylarginine and asymmetric dimethylarginine (MMA ADMA) in various substrates. It regulates numerous biological processes, including RNA metabolism, mRNA splicing, DNA damage repair, chromatin dynamics. The crystal structure of rat has been previously reported as a homodimer; however, higher-order oligomeric species human have observed vivo, their structural basis remains elusive. In...
Brain functions rely on synapses that are highly specialized cellular junctions connecting neurons. Synaptic scaffold proteins (e.g. liprin-α, ELKS, RIM and RIM-BP) orchestrate ion channels, receptors enzymes at presynaptic terminals to form active zones for neurotransmitter release. However, the underlying mechanism of zone assembly remains elusive. Here, we reported liprin-α proteins, core organizers zone, have a potential be oligomerized through N-terminal coiled-coil region. Our...