A5031701602- interferon and immune responses
- Viral Infections and Vectors
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Legionella and Acanthamoeba research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Animal Virus Infections Studies
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Immune Response and Inflammation
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Plant Virus Research Studies
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Viral Infections and Outbreaks Research
Fujian Normal University
2017-2025
Northwest A&F University
2022
Institute of Biophysics
2010-2021
Chinese Academy of Sciences
2012-2021
Qingdao National Laboratory for Marine Science and Technology
2019-2021
University of California, Los Angeles
1999-2017
Chinese Academy of Medical Sciences & Peking Union Medical College
2006-2012
Tsinghua University
2006
Self-assembling peptide (SAP) RADA16-I (Ac-(RADA)4-CONH2) has been suffering from a main drawback associated with low pH, which damages cells and host tissues upon direct exposure. In this study, we presented strategy to prepare nanofiber hydrogels two designer SAPs at neutral pH. was appended functional motifs containing cell adhesion RGD neurite outgrowth IKVAV. The were specially designed have opposite net charges the combination of created hydrogel (-IKVAV/-RGD) characterized by...
Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the Triticum monococcum CNL Sr35 directly recognizes effector AvrSr35 from Puccinia graminis f. sp . tritici and report a cryo–electron microscopy structure resistosome crystal AvrSr35. show forms homodimers are disassociated into monomers upon domain Sr35, which induces assembly...
Evolutionary arms race dynamics shape the diversity of viruses and their receptors. Identification key residues which are involved in interspecies transmission is important to predict potential pathogen spillover from wildlife humans. Previously, we have identified genetically diverse SARSr-CoVs Chinese horseshoe bats. Here, show highly polymorphic ACE2 bat populations. These variants support SARS-CoV SARSr-CoV infection but with different binding affinities spike proteins. The higher...
Abstract Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report detailed structural and functional analysis of uncultured Ruminococcus sp . Cas13d (UrCas13d)-crRNA complex. Two hydrated Mg 2+ ions aid stabilizing conformation crRNA repeat region. Sequestration divalent metal does not alter pre-crRNA processing, but abolishes target cleavage by UrCas13d. Notably, processing executed HEPN-2 domain....
Mangrove swamp is one of the world's richest and most productive marine ecosystems. This ecosystem also has a great ecological importance, but highly susceptible to anthropogenic disturbances. The balance mangrove depends largely on microbial communities in sediments. Thus, understanding how respond spatial differences essential for more accurate assessment health. To this end, we performed first medium-distance (150 km) research biogeographic distribution communities. hypervariable regions...
Abstract The emerging SARS-CoV-2 variants, commonly with many mutations in S1 subunit of spike (S) protein are weakening the efficacy current vaccines and antibody therapeutics. This calls for variant-proof targeting more conserved regions S protein. Here, we designed a recombinant vaccine, HR121, HR1 domain S2 HR121 consisting HR1–linker1–HR2–linker2–HR1, is conformationally functionally analogous to present fusion intermediate conformation subunit. Immunization rabbits rhesus macaques...
Argonaute (Ago) proteins are ubiquitous across all kingdoms of life. Eukaryotic Agos (eAgos) use small RNAs to recognize transcripts for RNA silencing in eukaryotes. In contrast, the functions prokaryotic counterparts (pAgo) less well known. Recently, short pAgos conjunction with associated TIR or Sir2 (SPARTA SPARSA) were found serve as antiviral systems combat phage infections. Herein, we present cryo-EM structures nicotinamide adenine dinucleotide (NAD
Abstract The intracellular bacterial pathogen Legionella pneumophila modulates host cell functions by secreting multiple effectors with diverse biochemical activities. In particular, of the SidE family interfere protein ubiquitination in a process that involves production phosphoribosyl ubiquitin (PR-Ub). Here, we show effector LnaB converts PR-Ub into ADP-ribosylated ubiquitin, which is further processed to ADP-ribose and functional (ADP-ribosyl)hydrolase MavL, thus maintaining homeostasis...
Abstract SARS‐CoV‐2 variants are under continuous emergence carry numerous mutations within S1 subunit in spike protein and have escaped neutralization through many currently used vaccines antibodies. The development of next‐generation is a continuing long‐term need. In our prior research, the recombinant vaccine HR121 targeting heptad repeat (HR) 1 domain S2 was constructed, which could evoke highly broad‐spectrum neutralizing antibodies vivo confer efficient protective effect on several...
The lysin LysGH15, which is derived from the staphylococcal phage GH15, demonstrates a wide lytic spectrum and strong activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we find that of full-length LysGH15 its CHAP domain dependent on calcium ions. To elucidate molecular mechanism, structures three individual domains were determined. Unexpectedly, crystal structure reveals an "EF-hand-like" calcium-binding site near Cys-His-Glu-Asn quartet active groove. date, in...
Severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel bunyavirus (SFTSV). Lack of vaccines and inadequate therapeutic treatments have made the spread virus global concern. Viral nucleocapsid protein (N) essential for its transcription replication. Here, we present crystal structures N from SFTSV homologs Buenaventura (BUE) Granada (GRA) viruses. The reveal that phleboviral folds into compact core domain extended N-terminal arm mediates...
Abstract Nucleotide oligomerization domain–like receptors (NLRs) and RIG-I–like (RLRs) detect diverse pathogen-associated molecular patterns to activate the innate immune response. The role of mammalian NLR NOD1 in sensing bacteria is well established. Although several studies suggest also plays a viruses, mechanisms behind this are still largely unknown. In study, we report on synergism antagonism between MDA5 isoforms teleost. zebrafish, overexpression enhances antiviral response mRNA...