A5059545838- Lung Cancer Research Studies
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Cancer therapeutics and mechanisms
- Hippo pathway signaling and YAP/TAZ
- Fibroblast Growth Factor Research
- Neuroendocrine Tumor Research Advances
- RNA modifications and cancer
- interferon and immune responses
- Developmental Biology and Gene Regulation
- Wnt/β-catenin signaling in development and cancer
- Neonatal Respiratory Health Research
- Hedgehog Signaling Pathway Studies
- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Adenosine and Purinergic Signaling
- Microtubule and mitosis dynamics
- Galectins and Cancer Biology
- Congenital heart defects research
- Mitochondrial Function and Pathology
Second Affiliated Hospital of Zhejiang University
2020-2025
Zhejiang University
2016-2025
Zhejiang Cancer Hospital
2025
National Human Genome Research Institute
2008-2018
Chinese Academy of Sciences
2000-2017
University of California, San Francisco
2012-2017
Institute of Mountain Hazards and Environment
2017
National Institutes of Health
2008-2015
The Military General Hospital of Beijing PLA
2015
MRC Laboratory of Molecular Biology
2004-2011
Control of organ size by cell proliferation and survival is a fundamental developmental process, its deregulation leads to cancer. However, the molecular mechanism underlying control remains elusive in vertebrates. In Drosophila , Hippo (Hpo) signaling pathway controls both restricting growth promoting death. Here we investigated whether mammals also require Hpo adult tissue homeostasis. We found that Mst1 Mst2 two mouse homologs sizes some, but not all organs, mice, act as tumor suppressors...
Pulmonary neuroendocrine cells (PNECs) are proposed to be the first specialized cell type appear in lung, but their ontogeny remains obscure. Although studies of PNECs have suggested involvement a number lung functions, neither vivo significance nor molecular mechanisms underlying them been elucidated. Importantly, long speculated constitute origin human small-cell cancer (SCLC) and recent mouse models support this hypothesis. However, genetic system that permits tracing early events PNEC...
Chondrocyte fate determination and maintenance requires Sox9, an intrinsic transcription factor, but is inhibited by Wnt/beta-catenin signaling activated extrinsic Wnt ligands. Here we explored the underlying molecular mechanism which Sox9 antagonizes in chondrocyte differentiation. We found that employed two distinct mechanisms to inhibit signaling: N terminus necessary sufficient promote beta-catenin degradation, whereas C required transcriptional activity without affecting its stability....
Identifying cells of tumor origin is a fundamental question in biology. Answers to this central will not only advance our understanding initiation and progression but also have important therapeutic implications. In study, we aimed uncover the lung adenocarcinoma, major subtype non-small cell cancer. To end, developed new mouse models adenocarcinoma that enabled selective manipulation gene activity surfactant associated protein C (SPC)-expressing cells, including alveolar type II...
YAP confers small cell lung cancer with the intratumoral heterogeneity and resistance to chemotherapy drugs.
Mesoderm formation and subsequent anterior-posterior (A-P) axis elongation are fundamental aspects of gastrulation, which is initiated by the primitive streak (PS). Convergent extension (CE) movements epithelial-mesenchymal transition (EMT) important for A-P in vertebrate embryos. The evolutionarily conserved planar cell polarity (PCP) pathway regulates CE, Wnts regulate many gastrulation including CE EMT. However, Wnt ligands that mammalian development remain unknown. Wnt11 Wnt5a lower...
Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how status IRF3 activation controlled remains elusive. Through functional screen human kinome, we found that mammalian sterile 20-like kinase 1 (Mst1), but not Mst2, profoundly inhibited cytosolic nucleic acid sensing. Mst1 associated...
Shear stress exerted by the blood stream modulates endothelial functions through altering gene expression. KLF2 and KLF4, mechanosensitive transcription factors, are promoted laminar flow to maintain homeostasis. However, how expression of KLF2/4 is regulated shear poorly understood. Here, we showed that activation PIEZO1 upregulates in cells. Mice with endothelial-specific deletion Piezo1 exhibit reduced thoracic aorta pulmonary vascular Mechanistically, activates PIEZO1, which results a...
Abstract Our previous study revealed that PI3K/AKT/mTOR signaling was associated with SCLC radioresistance. SBC2 cells were used as primary radioresistance models, while H446 continuously exposed to ionizing radiation (IR) develop acquired Cell viability and apoptosis assays investigate synergistic effects of BEZ235/GSK2126458 IR in vitro, immunoblotting, metabolite quantitative analysis bioinformatic analyses utilized explore the underlying mechanism. Both genetically engineered mouse...
Abstract Production of an appropriate number distinct cell types in precise locations during embryonic development is critical for proper tissue function. Homeostatic renewal or repair damaged tissues adults also requires expansion and transdifferentiation to replenish lost cells. However, the responses diverse injury are not fully elucidated. Moreover, molecular mechanisms underlying remain poorly understood. This knowledge essential harnessing regenerative potential individual types. study...
ABSTRACT Embryonic morphogenesis of a complex organism requires proper regulation patterning and directional growth. Planar cell polarity (PCP) signaling is emerging as crucial evolutionarily conserved mechanism whereby information conveyed. PCP thought to be established by global cues, recent studies have revealed an instructive role Wnt gradient in epithelial tissues both invertebrates vertebrates. However, it remains unclear whether Wnt/PCP regulated coordinated manner with embryonic...
The initiation and morphogenesis of cutaneous appendages depend on a series reciprocal signaling events between the epithelium mesenchyme embryonic skin. In development feather germs, early dermal signals induce formation epidermal placodes that in turn signal mesoderm to form condensations immediately beneath them. We find spatially temporally restricted pattern transcription for genes encode fibroblast growth factor (FGF) 2 FGF receptor (FGFR) 1 developing germs chicken embryo. FGF-2...
To reveal new tumor markers and target genes from differentially expressed of primary samples using cDNA microarray.The (33 )P labeled cDNAs were synthesized by reverse transcription message RNA the liver cancerous tissue adjacent non-cancerous same patient used to hybridize LifeGrid 1.0 microarray blot containing 8400 known unique human gene targets, an expression profile was produced in one paired tissue. After a global analysis genes, we selected some confirm differential Northern...
Human chromosome 8p23 is known as a region that associated with loss of heterozygosity (LOH), which frequently deleted in hepatocellular carcinoma (HCC) tissues. We report here the characterization gene for liver-related putative tumor suppressor (LPTS) localized at 8p23, was isolated by allelic-loss mapping and positional candidate cloning. The expression LPTS ubiquitous normal human tissues, albeit relatively low levels, whereas levels appeared to be significantly reduced, or sometimes...
Hedgehog (Hh) pathway plays a pivotal role in diverse aspects of development and postnatal physiology. Perturbation Hh signaling activation GLI1 (glioma-associated oncogene 1), dedicated transcription factor for pathway, are highly associated with several cancers, such as medulloblastoma basal cell carcinoma. Dynamic precise control activity is thus important to ensure proper homeostasis tumorigenesis. Here we show that MEKK2 (MAP3K2) MEKK3 (MAP3K3) inhibit transcriptional oncogenic function...
Abstract Necroptotic cell death is mediated by a super-molecular complex called necrosome which consists of receptor-interacting protein kinase 1 and 3 (RIPK1, RIPK3) mixed-lineage domain-like (MLKL). The role these kinases has been extensively investigated in the regulation necroptosis. However, whether phosphatase involved necroptosis still largely unknown. Here, we identified 6 catalytic subunit (PPP6C) promotes TNF-induced genome-wide CRISPR/Cas9 library screening. We found that PPP6C...
Oncolytic viruses have recently been proven to be an effective and promising cancer therapeutic strategy, but there is rare data about oncolytic therapy in esophageal squamous cell carcinoma (ESCC), especially measles virotherapy. Therefore, this study aimed explore whether the recombinant virus vaccine strain rMV-Hu191 has effect against ESCC cells vitro vivo elucidate underlying mechanisms. Our results showed that could efficiently replicate kill through caspase-3/GSDME-mediated...