A5090350551- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Amino Acid Enzymes and Metabolism
- Protein Structure and Dynamics
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Biochemical Acid Research Studies
- Acute Myeloid Leukemia Research
- Computational Drug Discovery Methods
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
- Enzyme Structure and Function
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Photosynthetic Processes and Mechanisms
- vaccines and immunoinformatics approaches
University of Bari Aldo Moro
2016-2025
Health and Human Development (2HD) Research Network
2023
University of Arizona
2023
Laboratoire de Biochimie
2020
CTI BioPharma (Italy)
2019
John Innes Centre
2010
National Research Council
2010
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1), specific to neurons and muscle, supplies aspartate the cytosol and, as a component of malate-aspartate shuttle, enables oxidation cytosolic NADH, thought be important in providing energy for central nervous system. We describe AGC1 deficiency, novel syndrome characterized by arrested psychomotor development, hypotonia, seizures child with homozygous missense mutation solute family 25, member 12, gene SLC25A12, which encodes...
Abstract Artichoke (Cynara cardunculus subsp. scolymus) extracts have high antioxidant capacity, due primarily to flavonoids and phenolic acids, particularly chlorogenic acid (5-caffeoylquinic [CGA]), dicaffeoylquinic caffeic acid, which are abundant in flower bracts bioavailable humans the diet. The synthesis of CGA can occur following different routes plant species, hydroxycinnamoyl-coenzyme A transferases important enzymes these pathways. Here, we report on isolation characterization two...
The recent severe acute respiratory syndrome, known as Coronavirus Disease 2019 (COVID-19) has spread so much rapidly and severely to induce World Health Organization (WHO) declare a state of emergency over the new coronavirus SARS-CoV-2 pandemic. While several countries have chosen almost complete lock-down for slowing down spread, scientific community is called respond devastating outbreak by identifying tools diagnosis treatment dangerous COVID-19. With this aim, we performed an in silico...
M1 macrophages enter a glycolytic state when endogenous nitric oxide (NO) reprograms mitochondrial metabolism by limiting aconitase 2 and pyruvate dehydrogenase (PDH) activity. Here, we provide evidence that NO targets the PDH complex using lipoate to generate nitroxyl (HNO). E2-associated is modified in NO-rich while E3 enzyme, also known as dihydrolipoamide (DLD), irreversibly inhibited. Mechanistically, show facilitates NO-mediated production of HNO, which interacts with thiols forming...
Background and Objective: Congenital myasthenic syndromes are rare inherited disorders characterized by fatigable weakness caused malfunction of the neuromuscular junction. We performed whole exome sequencing to unravel genetic aetiology in an
Flavoprotein oxidoreductases are members of a large protein family specialized dehydrogenases, which include type II NADH dehydrogenase, pyridine nucleotide-disulphide oxidoreductases, ferredoxin-NAD+ reductases, oxidases, and peroxidases, playing crucial role in the metabolism several prokaryotes eukaryotes. Although studies have been performed on single or subgroups flavoprotein comprehensive analysis structure-function relationships among different this great dehydrogenase is still...
In Saccharomyces cerevisiae, alpha-isopropylmalate (alpha-IPM), which is produced in mitochondria, must be exported to the cytosol where it required for leucine biosynthesis. Recombinant and reconstituted mitochondrial oxalacetate carrier (Oac1p) efficiently transported alpha-IPM addition its known substrates oxalacetate, sulfate, malonate contrast other di- tricarboxylate transporters as well previously proposed transporter. Transport was saturable with a half-saturation constant of 75 +/-...
Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is an autosomal recessive disorder of the urea cycle. With exception French-Canadian founder effect, no common mutation has been detected in other populations. In this study, we collected 16 additional HHH cases and expanded spectrum SLC25A15/ORC1 mutations. Eleven novel mutations were identified including six new missense one microrearrangement. We also measured transport properties recombinant purified proteins reconstituted...
<h3>Background</h3> Agenesis of corpus callosum has been associated with several defects the mitochondrial respiratory chain and citric acid cycle. We now report results biochemical molecular studies a patient severe neurodevelopmental disease manifesting by agenesis optic nerve hypoplasia. <h3>Methods results</h3> A was suspected in this based on prominent excretion 2-hydroxyglutaric Krebs cycle intermediates urine finding increased reactive oxygen species content decreased membrane...
Revealing geometric constraints that drive protein folding is a major challenge in computational biology.
Article4 September 2020Open Access Source DataTransparent process Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages Alessio Menga orcid.org/0000-0002-2827-5298 Laboratory of Tumor Inflammation Angiogenesis, Center for Cancer Biology (CCB), VIB, Leuven, Belgium Department Oncology, KU Molecular Biotechnology Health Science, Centre, University Torino, Italy Biosciences, Biotechnologies Biopharmaceutics, Bari, Search more papers this author...