A5080278477- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Erythrocyte Function and Pathophysiology
- Light effects on plants
- Drug Transport and Resistance Mechanisms
- Sphingolipid Metabolism and Signaling
- Molecular Sensors and Ion Detection
- Amino Acid Enzymes and Metabolism
- Lipid Membrane Structure and Behavior
- Photochromic and Fluorescence Chemistry
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- Transgenic Plants and Applications
- Ferrocene Chemistry and Applications
- Cell Adhesion Molecules Research
- Plant Physiology and Cultivation Studies
- Microtubule and mitosis dynamics
- Chemical Synthesis and Analysis
- Plant Gene Expression Analysis
- Nuclear Structure and Function
Austrian Academy of Sciences
2023-2025
CeMM Research Center for Molecular Medicine
2023-2025
Institute of Science and Technology Austria
2019-2023
Central European Institute of Technology
2015-2019
Masaryk University
2015-2019
Central European Institute of Technology
2017
Central European Institute of Technology – Masaryk University
2017
Czech Academy of Sciences, Institute of Experimental Botany
2013
Abstract Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in variety subcellular membranes. Loss-of-function an unusually high proportion SLC is genetically associated with plethora human diseases, making SLCs rapidly emerging but challenging drug target class. Knowledge their environment may elucidate the molecular basis for functional...
Sphingosine-1-phosphate (S1P) is a signaling lysolipid critical to heart development, immunity, and hearing. Accordingly, mutations in the S1P transporter SPNS2 are associated with reduced white cell count hearing defects. also exports S1P-mimicking FTY720-P (Fingolimod) thereby central pharmacokinetics of this drug when treating multiple sclerosis. Here, we use combination cryo-electron microscopy, immunofluorescence, vitro binding vivo export assays, molecular dynamics simulations probe...
Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception importance spatially heterogeneous distribution continue be debated. Here we show that by plasma membrane is an effective additional path for response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches...
Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of effects, in particular those associated with rapid responses. A possible candidate for component additional mechanisms AUXIN BINDING PROTEIN 1 (ABP1), whose function planta remains unclear. Here we combined expression analysis gain- loss-of-function approaches to analyze role ABP1 development. shows broad largely overlapping with, not regulated by,...
TPX2 performs multiple roles in microtubule organization. Previously, it was shown that plant AtTPX2 binds AtAurora1 kinase and colocalizes with microtubules a cell cycle-specific manner. To elucidate the function of further, this work analysed Arabidopsis cells overexpressing AtTPX2-GFP. Distinct arrays bundled microtubules, decorated AtTPX2-GFP, were formed vicinity nuclear envelope nuclei cells. The microtubular showed reduced sensitivity to anti-microtubular drugs. TPX2-mediated...
Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in variety subcellular membranes. Loss-of-function an unusually high proportion SLC is genetically associated with plethora human diseases, making SLCs rapidly emerging but challenging drug target class. Knowledge their environment may elucidate the molecular basis for functional integration...
SUMMARY Phytohormone auxin and its directional transport mediate much of the remarkably plastic development higher plants. Positive feedback between signaling is a key prerequisite for (i) self-organizing processes including vascular tissue formation (ii) growth responses such as gravitropism. Here we identify mechanism, by which directly targets PIN transporters. Via cell-surface ABP1-TMK1 receptor module, rapidly induces phosphorylation thus stabilization PIN2. Following gravistimulation,...
Multistep phosphorelay (MSP) cascades mediate responses to a wide spectrum of stimuli, including plant hormonal signaling, but several aspects MSP await elucidation. Here, we provide first insight into the key step MSP-mediated phosphotransfer in eukaryotic system, phosphorylation receiver domain histidine kinase CYTOKININ-INDEPENDENT 1 (CKI1RD) from Arabidopsis thaliana. We observed that crystal structures free, Mg2+-bound, and beryllofluoridated CKI1RD (a stable analogue labile...
Cytokinins comprise a group of phytohormones with an organ-specific mode action. Although the mechanisms controlling complex networks cytokinin metabolism are partially known, role individual types in maintenance homeostasis remains unclear. Utilizing overproduction single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted endoplasmic reticulum, we post-synthetically modulated ribosides, proposed transport forms cytokinins. We observed asymmetric...
Abstract Sphingosine-1-phosphate (S1P) is a signaling lysolipid critical to heart development, immunity, and hearing. Accordingly, mutations in the S1P transporter SPNS2 are associated with reduced white cell count hearing defects. also exports S1P-mimicking FTY720-P (Fingolimod) thereby central pharmacokinetics of this drug when treating multiple sclerosis. Here, we use combination cryo-electron microscopy, immunofluorescence, vitro binding vivo export assays, molecular dynamic simulations...
Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience 1,2 . Although cytokinin receptors substantially localised to the endoplasmic reticulum 3–5 , cellular sites of perception continue be debated 1,6,7 Several types display bioactivity 8,9 recently a cell-specific gradient was reported roots 10 Yet, importance spatially heterogeneous distribution specific cytokinin(s) that account for different responses remain unclear. Here we show by plasma...
Abstract Auxin belongs among major phytohormones and governs multiple aspects of plant growth development. The establishment auxin concentration gradients, determines, other processes, organ positioning responses to environmental stimuli. Herein we report the synthesis new NBD- or DNS-labelled IAA derivatives elucidation their biological activity, fluorescence properties subcellular accumulation patterns in planta . These novel compounds did not show auxin-like but instead antagonized...