A5052072285- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Polysaccharides and Plant Cell Walls
- Allelopathy and phytotoxic interactions
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Plant Gene Expression Analysis
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Insect and Pesticide Research
- Fungal Plant Pathogen Control
- Fern and Epiphyte Biology
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Light effects on plants
- Plant-Microbe Interactions and Immunity
- Metabolism and Genetic Disorders
- Ethnobotanical and Medicinal Plants Studies
Czech Academy of Sciences, Institute of Experimental Botany
2019-2025
Palacký University Olomouc
2019-2025
Umeå Plant Science Centre
2018-2025
Swedish University of Agricultural Sciences
2018-2025
Umeå University
2018-2022
University of Parma
2013-2021
Indole-3-acetic acid (IAA), the most common form of auxin, is involved in a great range plant physiological processes. IAA synthesized from amino tryptophan and can be transported inactivated myriad ways. Despite intense research efforts, there are still dark corners our comprehension metabolism its interplays with other pathways. Genetic screens powerful tool for unbiasedly looking new players given biological process. However, pleiotropism auxin-related phenotypes indirect effects make it...
In recent years, substantial progress has been made in exploring auxin conjugation and metabolism, primarily aiming at indole-3-acetic acid (IAA). However, the metabolic regulation of another key auxin, phenylacetic (PAA), remains largely uncharacterized. Here, we provide a comprehensive exploration PAA metabolism land plants. Through LC-MS screening across multiple plant species their organs, identified four previously unreported endogenous metabolites: phenylacetyl-leucine (PAA-Leu),...
Vegetative propagation relies on the capacity of plants to regenerate
Summary Dynamic regulation of the concentration natural auxin (IAA) is essential to coordinate most physiological and developmental processes responses environmental changes. Oxidation IAA a major pathway control concentrations in angiosperms and, along with conjugation, respond perturbation homeostasis. However, these regulatory mechanisms remain poorly investigated conifers. To reduce this knowledge gap, we different contributions inactivation pathways MS‐based quantification metabolites...
Abstract Background Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an important insight into ongoing processes is essential for biological studies performed on Results high-throughput sample preparation method liquid chromatography–tandem mass spectrometry determination 25 acidic classed as auxins, jasmonates, abscisates salicylic acid was...
Abstract Together with auxin transport, metabolism is a key determinant of signaling output by plant cells. Enzymatic machinery involved in subject to regulation based on numerous inputs, including the concentration itself. Therefore, experiments characterizing altered availability and subsequent changes could elucidate function regulatory role individual elements metabolic machinery. Here, we studied auxin-dependent tobacco BY-2 We revealed that N-(2-oxindole-3-acetyl)-l-aspartic acid...
Recalcitrant adventitious root (AR) development is a major hurdle in propagating commercially important woody plants. Although significant progress has been made to identify genes involved subsequent steps of AR development, the molecular basis differences apparent recalcitrance form between easy-to-root and difficult-to-root genotypes remains unknown. To address this, we generated cambium tissue-specific transcriptomic data from stem cuttings hybrid aspen, T89 (difficult-to-root) poplar...
Abstract Jasmonates are a family of oxylipin phytohormones regulating plant development and growth mediating “defense versus growth” responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently CORONATIVE INSENSITIVE 1-mediated signaling in several stress-induced developmental processes. However, its perception metabolism only partially understood. An isoleucine analog the biologically active JA-Ile, OPDA-Ile, was detected years ago wounded...
Asexual propagation in Ceratonia siliqua L. (carob), species of economic value, is difficult because adventitious rooting recalcitrance. In Pinus radiata hypocotyl cuttings enhanced by two urea-derivatives, 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU) and 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU), combined with exogenous indole-3-butyric acid (IBA). The research was aimed to define the role these urea-derivatives root (AR) formation carob, identify morphogenic roles induced but also pine, a...
Cytokinin and auxin are plant hormones that coordinate many aspects of development. Their interactions in underground growth well established, occurring at the levels metabolism, signaling, transport. Unlike hormone classes, cytokinins represented by more than one active molecule. Multiple mutant lines, blocking specific parts cytokinin biosynthetic pathways, have enabled research plants with deficiencies cytokinin-types. While most these mutants confirmed impeding effect on root growth,...
Summary Seasonal dynamics of root growth play an important role in large‐scale ecosystem processes; they are largely governed by regulatory compounds and influenced environmental conditions. Yet, our knowledge about physiological drivers is mostly limited to laboratory‐based studies on model plant species. We sampled tips Eriophorum vaginatum analyzed their auxin concentrations meristem lengths biweekly over a growing season situ subarctic peatland, both surface soil at the permafrost...
AbstractIn order to conserve and multiply the aquatic fern Marsilea quadrifolia L., in a long-term vitro procedure, effects of different cytokinins, i.e., 6-benzylaminopurine, zeatine riboside, N6-(2-isopentenyl)adenine, were investigated, varying their concentration period supplementation. No clear stimulatory effect on de novo nodes produced per explant was detected when compared with hormone-free (HF) condition. On contrary, rhizome micropropagation inhibited, inhibition decreasing...
Plants rely on concentration gradients of the native auxin, indole-3-acetic acid (IAA), to modulate plant growth and development. Both metabolic transport processes participate in dynamic regulation IAA homeostasis. Free levels can be reduced by inactivation mechanisms, such as conjugation degradation. conjugated via ester linkage glucose, or amide amino acids, degraded oxidation. Members UDP glucosyl transferase (UGT) family catalyze conversion indole-3-acetyl-1-glucosyl (IAGlc); contrast,...
Abstract Jasmonates (JAs) are a family of oxylipin phytohormones regulating plant development and growth mediating ‘defense versus growth’ responses. The upstream JA biosynthetic precursor cis -(+)-12-oxo-phytodienoic acid ( -OPDA) has been reported to act independently the COI1-mediated signaling in several stress-induced developmental processes. However, its means perception metabolism only partially understood. Furthermore, -OPDA, but not JA, occurs non-vascular species, such as...
Abstract In recent years, substantial progress has been made in exploring auxin conjugation and metabolism, primarily aiming at indole-3-acetic acid (IAA). However, the metabolic regulation of another key auxin, phenylacetic (PAA), remains largely uncharacterized. Here, we provide a comprehensive exploration PAA metabolism land plants. Through LC-MS screening across multiple plant species their organs, identified four previously unreported endogenous metabolites: phenylacetyl-leucine...
AbstractAdansonia digitata L. (African baobab), is an important multi-purpose tree, whose distribution at present limited to wild or semi-domesticated individuals widespread in Africa. Its threatened by seedling clearance for other land use and potentially overharvesting induced growing commercial of baobab fruit. Recently, efforts have been made establish domestication conservation strategies, with mixed results due the low germinability seeds, a factor that hinders possibility developing...
Cis-(+)-12-oxophytodienoic acid (cis-(+)-OPDA) is a bioactive jasmonate, precursor of jasmonic acid, which also displays signaling activity on its own. Modulation cis-(+)-OPDA actions may be carried out via biotransformation leading to metabolites various functions, similar other phytohormones. This work introduces methodology for the synthesis racemic cis-OPDA conjugates with amino acids (OPDA-aa) and their deuterium-labeled analogs, enables identification accurate quantification these...