A5010902252- Ovarian cancer diagnosis and treatment
- Cancer Mechanisms and Therapy
- PARP inhibition in cancer therapy
- CAR-T cell therapy research
- Plant Molecular Biology Research
- vaccines and immunoinformatics approaches
- Single-cell and spatial transcriptomics
- Plant nutrient uptake and metabolism
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Plant biochemistry and biosynthesis
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Science, Research, and Medicine
- Cancer Cells and Metastasis
- Phytochemicals and Antioxidant Activities
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- Metabolomics and Mass Spectrometry Studies
- Cell Image Analysis Techniques
Ghent University
2020-2024
VIB Single Cell Core
2021-2024
Erasmus MC
2024
Ghent University Hospital
2024
VIB-UGent Center for Inflammation Research
2020-2024
Roots primed for better phosphate uptake Phosphate is a key resource plants, and remediating deficiency drives considerable fertilizer use. In low-phosphate conditions, roots make more root hairs, which makes them able to take up what little can be found. Wendrich et al. performed single-cell transcriptomics on the developing Arabidopsis queried resulting gene-expression atlas responses related vascular development. The authors found that signals regulating hair development began in inner...
In the plant meristem, tissue-wide maturation gradients are coordinated with specialized cell networks to establish various developmental phases required for indeterminate growth. Here, we used single-cell transcriptomics reconstruct protophloem trajectory from birth of progenitors terminal differentiation in
Abstract As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization leaf mesophyll epidermal cell layers orchestrates photosynthesis gas exchange. In addition, water nutrients for growth transported through tissue. To establish single-cell transcriptomic landscape these different tissues, we performed high-throughput transcriptome sequencing individual cells isolated from young Arabidopsis (Arabidopsis thaliana) seedlings...
In rare instances, pediatric SARS-CoV-2 infection results in a novel immunodysregulation syndrome termed multisystem inflammatory children (MIS-C). We compared MIS-C immunopathology with severe COVID-19 adults. does not result pneumocyte damage but is associated vascular endotheliitis and gastrointestinal epithelial injury. MIS-C, the cytokine release characterized by IFNγ type I interferon. Persistence of patrolling monocytes differentiates from COVID-19, which dominated HLA-DRlo classical...
Abstract Background Multiplexing of samples in single-cell RNA-seq studies allows a significant reduction the experimental costs, straightforward identification doublets, increased cell throughput, and sample-specific batch effects. Recently published multiplexing techniques using oligo-conjugated antibodies or -lipids allow barcoding cells, process called “hashing.” Results Here, we compare hashing performance TotalSeq-A -C antibodies, custom synthesized lipids MULTI-seq lipid hashes four...
Abstract OTULIN is a deubiquitinase that specifically cleaves linear ubiquitin chains. Here we demonstrate the ablation of Otulin selectively in keratinocytes causes inflammatory skin lesions develop into verrucous carcinomas. Genetic deletion Tnfr1 , knockin expression kinase-inactive Ripk1 or keratinocyte-specific Fadd and Mlkl completely rescues mice with deficiency from dermatitis tumorigenesis, thereby identifying keratinocyte cell death as driving force for inflammation. Single-cell...
Despite the scientific and economic importance of maize, little is known about its specialized metabolism. Here, five maize organs were profiled using different reversed-phase liquid chromatography-mass spectrometry methods. The resulting spectral metadata, combined with candidate substrate-product pair (CSPP) networks, allowed structural characterization 427 5,420 compounds, including phenylpropanoids, flavonoids, benzoxazinoids, auxin-related among others. Only 75 compounds already...
Abstract High-grade serous ovarian cancer (HGSOC) is responsible for the largest number of deaths. The frequent therapy-resistant relapses necessitate a better understanding mechanisms driving therapy resistance. Therefore, we mapped more than hundred thousand cells HGSOC patients in different phases disease, using single-cell RNA sequencing. Within patients, compared chemonaive with chemotreated samples. As such, were able to create atlas lesions and their treatment. This revealed high...
The long-term effectiveness of immunotherapies against Multiple Myeloma (MM) remains elusive, demonstrated by the inevitable relapse in patients. This underscores urgent need for an in-depth analysis MM tumor-immune microenvironment (TME). Hereto, a representative immunocompetent mouse model can offer valuable approach to study dynamic changes within MM-TME and uncover potential resistance mechanisms hampering effective durable therapeutic strategies MM. We generated comprehensive...
Abstract The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed protophloem developmental trajectory of 19 from birth terminal differentiation at single resolution Arabidopsis root. We found cellular specification is mediated near stem niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, PEAR dependent program repressed a broad gradient...
Abstract Single-cell RNA sequencing is instrumental to unravel the cellular and transcriptomic heterogeneity of T B cells in health disease. Recent technological advances add additional layers information allowing researchers simultaneously explore transcriptomic, surface protein immune receptor diversity during adaptive responses. The increasing data complexity poses a burden on workload for bioinformaticians, who are often not familiar with specificities biology profiling. wet-lab...
ABSTRACT Since the precursor frequency of naïve T cells is extremely low, investigating early steps antigen-specific cell activation challenging. To overcome this detection problem, adoptive transfer a cohort purified from receptor (TCR) transgenic donors has been extensively used but not readily available for emerging pathogens. Constructing TCR mice hybridomas labor-intensive and sometimes erratic process, since best clones are selected based on antigen-induced CD69 upregulation or IL-2...
Since the precursor frequency of naive T cells is extremely low, investigating early steps antigen-specific cell activation challenging. To overcome this detection problem, adoptive transfer a cohort purified from receptor (TCR) transgenic donors has been extensively used but not readily available for emerging pathogens. Constructing TCR mice hybridomas labor-intensive and sometimes erratic process, since best clones are selected based on antigen-induced CD69 upregulation or IL-2 production...
Since the precursor frequency of naïve T cells is extremely low, investigating early steps antigen-specific cell activation challenging. To overcome this detection problem, adoptive transfer a cohort purified from receptor (TCR) transgenic donors has been extensively used but not readily available for emerging pathogens. Constructing TCR mice hybridomas labor-intensive and sometimes erratic process, since best clones are selected based on antigen-induced CD69 upregulation or IL-2 production...
Since the precursor frequency of naïve T cells is extremely low, investigating early steps antigen-specific cell activation challenging. To overcome this detection problem, adoptive transfer a cohort purified from receptor (TCR) transgenic donors has been extensively used but not readily available for emerging pathogens. Constructing TCR mice hybridomas labor-intensive and sometimes erratic process, since best clones are selected based on antigen-induced CD69 upregulation or IL-2 production...
<title>Abstract</title> Dendritic cells (DCs) are short-lived immune that continuously roam our body in search for foreign or self-antigens. Upon acquisition of antigen, they mature and start migrating to the lymph node present antigen naïve T cells. Depending on context wherein is acquired, DCs will a homeostatic immunogenic manner. So far, field lacking proper tools distinguish between two maturation states. Most markers shared states therefore inappropriate use. Still, defining type...
Since the precursor frequency of naive T cells is extremely low, investigating early steps antigen-specific cell activation challenging. To overcome this detection problem, adoptive transfer a cohort purified from receptor (TCR) transgenic donors has been extensively used but not readily available for emerging pathogens. Constructing TCR mice hybridomas labor-intensive and sometimes erratic process, since best clones are selected based on antigen-induced CD69 upregulation or IL-2 production...