A5044755566- Immunotherapy and Immune Responses
- Immune cells in cancer
- Inflammasome and immune disorders
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Autophagy in Disease and Therapy
- Endometriosis Research and Treatment
- Single-cell and spatial transcriptomics
- Chemokine receptors and signaling
- Eosinophilic Esophagitis
- Parasites and Host Interactions
- Phagocytosis and Immune Regulation
- Parasitic Diseases Research and Treatment
VIB-UGent Center for Inflammation Research
2019-2025
Vrije Universiteit Brussel
2019-2025
Background Modulation and depletion strategies of regulatory T cells (Tregs) constitute valid approaches in antitumor immunotherapy but suffer from severe adverse effects due to their lack selectivity for the tumor-infiltrating (ti-)Treg population, indicating need a ti-Treg specific biomarker. Methods We employed single-cell RNA-sequencing mouse model non-small cell lung carcinoma (NSCLC) obtain comprehensive overview T-cell compartment, with focus on subpopulations. These findings were...
Macrophages are often prominently present in the tumor microenvironment, where distinct macrophage populations can differentially affect progression. Although metabolism influences function, studies on metabolic characteristics of ex vivo tumor-associated (TAM) subsets rather limited. Using transcriptomic and analyses, we now reveal that pro-inflammatory major histocompatibility complex (MHC)-IIhi TAMs display a hampered tricarboxylic acid (TCA) cycle, while reparative MHC-IIlo show higher...
IL1β is a central mediator of inflammation. Secretion typically requires proteolytic maturation by the inflammasome and formation membrane pores gasdermin D (GSDMD). Emerging evidence suggests an important role for in promoting cancer progression patients, but underlying mechanisms are ill-defined. Here, we have shown key driving tumor two distinct mouse models. Notably, activation inflammasome, caspase-8, as well pore-forming proteins GSDMD mixed lineage kinase domain-like protein host were...
Local delivery of mRNA-based immunotherapy offers a promising avenue as it enables the production specific immunomodulatory proteins that can stimulate immune system to recognize and eliminate cancer cells while limiting systemic exposure toxicities. Here, we develop employ lipid-based nanoparticles (LNPs) intratumorally deliver an mRNA mixture encoding cytokines interleukin (IL)−21 IL-7 immunostimulatory molecule 4-1BB ligand (Triplet LNP). IL-21 synergy with 4-1BBL leads profound increase...
Large peritoneal macrophages (LPMs) play a role as gatekeepers of homeostasis by providing first line defense against pathogens. A third the LPMs express surface receptor VSIG4, but it is unclear whether these cells differ from their VSIG4-negative counterparts and perform dedicated functions. We demonstrate that VSIG4+, not VSIG4-, are in majority derived embryonal precursors, occurrence largely independent sex microbiota increases with age. Although transcriptome proteome indistinguishable...
The long-term effectiveness of immunotherapies against Multiple Myeloma (MM) remains elusive, demonstrated by the inevitable relapse in patients. This underscores urgent need for an in-depth analysis MM tumor-immune microenvironment (TME). Hereto, a representative immunocompetent mouse model can offer valuable approach to study dynamic changes within MM-TME and uncover potential resistance mechanisms hampering effective durable therapeutic strategies MM. We generated comprehensive...
Conventional dendritic cells (cDCs) are at the forefront of activating immune system to mount an anti-tumor response. Flt3L is a cytokine required for DC development that can increase abundance in tumor when administered therapeutically. However, impact on phenotype distinct cDC subsets microenvironment still largely undetermined. Here, using multi-omic single-cell analysis, we show therapy increases all orthotopic E0771 and TS/A breast cancer LLC lung models, but this did not result...
Junctional adhesion molecule-A (JAM-A), expressed on the surface of myeloid cells, is required for extravasation at sites inflammation and may also modulate cell activation. Infiltration cells a common feature tumors that drives disease progression, but function JAM-A in this phenomenon its impact tumor-infiltrating little understood. Here we show systemic cancer-associated mice enhanced expression selectively circulating monocytes an IL1β-dependent manner. Using myeloid-specific...
Large peritoneal macrophages (LPMs) play a role as gatekeepers of homeostasis by providing first line defense against pathogens. A third the LPMs express surface receptor VSIG4, but it is unclear whether these cells differ from their VSIG4-negative counterparts and perform dedicated functions. We demonstrate that VSIG4+, not VSIG4-, are in majority derived embryonal precursors occurrence largely independent sex microbiota. Although transcriptome proteome indistinguishable VSIG4- at...
<div>Abstract<p>IL1β is a central mediator of inflammation. Secretion IL1β typically requires proteolytic maturation by the inflammasome and formation membrane pores gasdermin D (GSDMD). Emerging evidence suggests an important role for in promoting cancer progression patients, but underlying mechanisms are ill-defined. Here, we have shown key driving tumor two distinct mouse models. Notably, activation inflammasome, caspase-8, as well pore-forming proteins GSDMD mixed lineage...
<p>Supplementary Figures 1-11</p>
<p>Supplementary Figures 1-11</p>
<div>Abstract<p>IL1β is a central mediator of inflammation. Secretion IL1β typically requires proteolytic maturation by the inflammasome and formation membrane pores gasdermin D (GSDMD). Emerging evidence suggests an important role for in promoting cancer progression patients, but underlying mechanisms are ill-defined. Here, we have shown key driving tumor two distinct mouse models. Notably, activation inflammasome, caspase-8, as well pore-forming proteins GSDMD mixed lineage...
Macrophages are often prominently present in the tumor microenvironment, where distinct macrophage populations can differentially affect progression. Although metabolism of macrophages influences their function, little is known about metabolic characteristics tumor-associated (TAM) subsets. Using transcriptomic and metabolomic analyses, we now reveal that pro-inflammatory MHC-IIhi TAMs display a hampered TCA cycle, while reparative MHC-IIlo show higher oxidative glycolytic metabolism. Both...