Niels Vandamme
A5012268585- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Biosimilars and Bioanalytical Methods
- Chemokine receptors and signaling
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- PARP inhibition in cancer therapy
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Cancer Immunotherapy and Biomarkers
- Acute Lymphoblastic Leukemia research
- melanin and skin pigmentation
- Melanoma and MAPK Pathways
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
Ghent University
2015-2024
VIB-VUB Center for Structural Biology
2022-2024
VIB Single Cell Core
2021-2024
VIB-UGent Center for Inflammation Research
2015-2024
Cancer Research Institute Ghent
2017-2024
VIB-KU Leuven Center for Cancer Biology
2023-2024
Guangzhou University
2023
University of Lausanne
2023
National Botanical Research Institute
2023
Council of Scientific and Industrial Research
2023
Thymus development, cell by The human thymus is the organ responsible for maturation of many types T cells, which are immune cells that protect us from infection. However, it not well known how these develop with a full complement contains necessary variation to variety pathogens. By performing single-cell RNA sequencing on more than 250,000 Park et al. examined changes occur in over course life. They found development occurs coordinated manner among and their developmental microenvironment....
Metabolic-associated fatty liver disease (MAFLD) represents a spectrum of states ranging from simple steatosis to non-alcoholic steatohepatitis (NASH). Hepatic macrophages, specifically Kupffer cells (KCs), are suggested play important roles in the pathogenesis MAFLD through their activation, although exact played by these remain unclear. Here, we demonstrated that KCs were reduced being replaced macrophages originating bone marrow. Recruited existed two subsets with distinct activation...
Roots primed for better phosphate uptake Phosphate is a key resource plants, and remediating deficiency drives considerable fertilizer use. In low-phosphate conditions, roots make more root hairs, which makes them able to take up what little can be found. Wendrich et al. performed single-cell transcriptomics on the developing Arabidopsis queried resulting gene-expression atlas responses related vascular development. The authors found that signals regulating hair development began in inner...
Highlights•ZEB2 is highly expressed across the macrophage lineage•ZEB2 preserves tissue-specific identities of macrophages tissues•ZEB2 deficient are outcompeted by WT counterparts•LXRα crucial for Kupffer cell identity and maintained ZEB2SummaryHeterogeneity between different populations has become a defining feature this lineage. However, conserved factors remain largely unknown. The transcription factor ZEB2 best described its role in epithelial to mesenchymal transition; however, within...
Background Modulation and depletion strategies of regulatory T cells (Tregs) constitute valid approaches in antitumor immunotherapy but suffer from severe adverse effects due to their lack selectivity for the tumor-infiltrating (ti-)Treg population, indicating need a ti-Treg specific biomarker. Methods We employed single-cell RNA-sequencing mouse model non-small cell lung carcinoma (NSCLC) obtain comprehensive overview T-cell compartment, with focus on subpopulations. These findings were...
Natural killer (NK) cell maturation is a tightly controlled process that endows NK cells with functional competence and the capacity to recognize target cells. Here, we found transcription factor (TF) Zeb2 was most highly induced TF during maturation. known control epithelial mesenchymal transition, but its role in immune mostly undefined. Targeted deletion of resulted impaired maturation, survival, exit from bone marrow. function preserved, mice lacking were more susceptible B16 melanoma...
Abstract As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization leaf mesophyll epidermal cell layers orchestrates photosynthesis gas exchange. In addition, water nutrients for growth transported through tissue. To establish single-cell transcriptomic landscape these different tissues, we performed high-throughput transcriptome sequencing individual cells isolated from young Arabidopsis (Arabidopsis thaliana) seedlings...
In rare instances, pediatric SARS-CoV-2 infection results in a novel immunodysregulation syndrome termed multisystem inflammatory children (MIS-C). We compared MIS-C immunopathology with severe COVID-19 adults. does not result pneumocyte damage but is associated vascular endotheliitis and gastrointestinal epithelial injury. MIS-C, the cytokine release characterized by IFNγ type I interferon. Persistence of patrolling monocytes differentiates from COVID-19, which dominated HLA-DRlo classical...
Dendritic cells (DCs) mature in an immunogenic or tolerogenic manner depending on the context which antigen is perceived, preserving balance between immunity and tolerance. Whereas pathways driving maturation response to infectious insults are well-characterized, signals that drive during homeostasis still poorly understood. We found engulfment of apoptotic triggered homeostatic type 1 conventional DCs (cDC1s) within spleen. This process could be mimicked by empty, nonadjuvanted lipid...
Non-small cell lung cancer (NSCLC) is known for high relapse rates despite resection in early stages. Here, we present the results of a phase I clinical trial which dendritic (DC) vaccine targeting patient-individual neoantigens evaluated patients with resected NSCLC. Vaccine manufacturing feasible six 10 enrolled patients. Toxicity limited to grade 1-2 adverse events. Systemic T responses are observed five out vaccinated patients, remaining detectable up 19 months post vaccination....
Abstract Purpose: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease, affecting children and adults. Chemotherapy treatments show high response rates but have debilitating effects carry risk of relapse. Previous work implicated NOTCH1 other oncogenes. However, direct inhibition these pathways affects healthy tissues cancer alike. Our goal in this has been to identify enzymes active T-ALL whose activity could be targeted for therapeutic purposes. Experimental Design: To...
Abstract Epithelial-to-mesenchymal transition (EMT)-inducing transcription factors (TF) are well known for their ability to induce mesenchymal states associated with increased migratory and invasive properties. Unexpectedly, nuclear expression of the EMT-TF ZEB2 in human primary melanoma has been shown correlate reduced invasion. We report here that is required outgrowth melanomas metastases at secondary sites. Ablation Zeb2 hampered vivo, whereas ectopic enhanced proliferation growth both...
Abstract The thymus provides a nurturing environment for the differentiation and selection of T cells, process orchestrated by their interaction with multiple thymic cell types. We utilised single-cell RNA-sequencing (scRNA-seq) to create census human reconstruct T-cell trajectories receptor (TCR) recombination kinetics. Using this approach, we identified located in situ novel CD8αα + populations, fibroblast subtypes activated dendritic (aDC) states. In addition, reveal bias TCR selection,...