A5017318795- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Neuroblastoma Research and Treatments
- Cell Image Analysis Techniques
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Neonatal Respiratory Health Research
- Ferroptosis and cancer prognosis
- Acute Myeloid Leukemia Research
- Childhood Cancer Survivors' Quality of Life
- Meningioma and schwannoma management
- Vascular Malformations Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Ethics and Legal Issues in Pediatric Healthcare
- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- Adolescent and Pediatric Healthcare
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Research and Treatments
Universitair Ziekenhuis Leuven
2021-2025
KU Leuven
2020-2024
Sciensano (Belgium)
2024
Clinically relevant immunological biomarkers that discriminate between diverse hypofunctional states of tumor-associated CD8 + T cells remain disputed. Using multiomics analysis cell features across multiple patient cohorts and tumor types, we identified niche–dependent exhausted other types states. in “supportive” niches, like melanoma or lung cancer, exhibited reactivity–driven exhaustion (CD8 EX ). These included a proficient effector memory phenotype, an expanded receptor (TCR)...
Uterine leiomyosarcomas (uLMS) are aggressive tumours with poor prognosis and limited treatment options. Although immune checkpoint blockade (ICB) has proven effective in some 'challenging-to-treat' cancers, clinical trials showed that uLMS do not respond to ICB. Emerging evidence suggests aberrant PI3K/mTOR signalling can drive resistance We therefore explored the relevance of pathway for ICB pharmacological inhibition this sensitise these
Abstract Glioblastoma remains a highly malignant, inevitably recurring brain tumor with dismal prognosis. Tumor progression is influenced by cellular interactions between the and its microenvironment, yet quantitative data on these interactions, particularly under standard of care therapy, sparse. We utilized spatial, single-cell, multiome profiling to map therapy-induced changes in architecture >700 paired glioblastoma samples derived from 101 patients at diagnosis recurrence. This...
A randomized, double-blind, placebo-controlled trial investigating the efficacy and safety of systemic hydrocortisone treatment initiated 7 to 14 days after birth in ventilatordependent, very preterm infants found no significant difference composite primary outcome death or bronchopulmonary dysplasia at 36 weeks' postmenstrual age. 1 However, a preplanned exploratory analysis reduced risk age favor hydrocortisone.We examined prespecified follow-up neurodevelopmental impairment (NDI) 2 years'...
Abstract Children and Adolescents Young Adults with cancer represent a young population specific needs, which need to be addressed in patient- cancer-driven way. There is an urgent support extend the ongoing initiatives Belgium. First, multidisciplinary care programmes dedicated children reviewed, those for developed close collaboration between paediatric adult oncology haematology teams. This needs done considering entire patient journey; from prevention, diagnosis, treatment,...
Abstract The impact of aging on the immune landscape luminal breast cancer (Lum‐BC) is poorly characterized. Understanding age‐related dynamics editing in Lum‐BC anticipated to improve therapeutic benefit immunotherapy older patients. To this end, here we applied ‘multiple iterative labeling by antibody neo‐deposition’ (MILAN) technique, a spatially resolved single‐cell multiplex immunohistochemistry method. We created tissue microarrays sampling both tumor center and invasive front tumors...
ABSTRACT Tumoural-CD8 + T cells exhibit exhausted or dysfunctional states. Contrary to immunotherapy-responsive exhausted-CD8 cells, the clinical features of dysfunctional-CD8 are disputed. Hence, we conducted large-scale multi-omics and multi-dimensional mapping CD8 cell-states across multiple cancer patient-cohorts. This identified tumour-specific continuum 6 human cancers, partly imprinted by organ-specific immuno-modulatory niches. Herein, melanoma glioblastoma enriched prototypical (CD8...
Acute megakaryoblastic leukemia (AMKL) is a rare disease, occurring mostly in infants and young children. The chromosomal translocation t(1;22)(p13;q13), resulting the RBM15‑MKL1 fusion gene, recurrent diagnostic with AMKL. present case report describes of newborn girl, without Down's syndrome, congenital At birth, infant had hepatosplenomegaly peripheral blood count revealed anemia, thrombopenia leukocytosis, 28% blasts. Immunophenotyping demonstrated blasts positive for CD34, CD61 CD42b....
<title>Abstract</title> The authors have requested that this preprint be removed from Research Square.
Abstract Glioblastoma (GBM) remains a highly malignant, intrinsically resistant and inevitably recurring brain tumor with dismal prognosis. The aggressiveness lack of effective GBM treatments can be attributed to the heterogeneous plastic nature cells, which easily confer resistance standard-of-care (SOC) therapy. While progression has also been interactions microenvironment, quantitative data describing these are still largely missing. Here, we used an multiome approach combining...
Abstract Introduction Diffuse intrinsic pontine glioma is a rare disease with high mortality. Our primary aim was to determine the incidence of this in Belgium. Secondly, we wanted compare treatment approach Belgian pediatric oncology centres, investigate possibilities for improvement. Methods We retrospectively collected and analysed data on DIPG-patients diagnosed between 1994 2018 recorded Cancer Registry. included patients ≤ 18 years who were followed one eight centres. Results 100 over...
e15089 Background: The ‘immune landscape’ of luminal breast tumors is poorly described to date. We applied the ‘multiple iterative labeling by antibody ‘neodeposition’ (MILAN) technique for in-depth characterization tumor microenvironment luminal-like cancer (BC) in relation age. Methods: Newly diagnosed patients with early BC (grade 2/3, ER+, HER2-, size ≥1,5 cm) were prospectively included UH Leuven between March 2014 and November 2015 divided into 3 distinct age categories (young: 35-45...
Abstract Diffuse leptomeningeal glioneuronal tumors (DLGNT) are rare neoplasms of the central nervous system and have been included in 2016 update WHO classification. This is first description a DLGNT disseminating to peritoneal cavity via ventriculoperitoneal shunt (VPS) child. We describe an 11-year old girl who received VPS for Dandy-Walker malformation at age seven, was diagnosed with spinal pilocytic astrocytoma metastases six months later. She chemotherapy (SIOP-LGG protocol) partial...
Abstract Diffuse leptomeningeal glioneuronal tumors (DLGNT) are rare neoplasms of the central nervous system. We describe generation patient-derived models from a DLGNT that metastasized to peritoneal cavity via ventriculoperitoneal shunt in child. The original tumor contained KIAA1549:BRAF fusion with chromosome 1p deletion and corresponded methylation subclass DLGNT-MC-2 From sample ascitic fluid, metastatic tumoral cells could be extracted expanded ex vivo into long-term cell culture...
Abstract Brain tumors are still a major cause of morbidity and mortality in children, despite extensive research. An individualized therapy is warranted to combat the heterogeneity present these tumors. Therefore, this study aims at developing patient-derived models from both low- high-grade As such, can be further characterized treatment sensitivities studied. All pediatric patients diagnosed with brain tumor University Hospitals Leuven receiving surgical intervention were included after...
Abstract High-grade glioma are the main cause of cancer-related death in children. The highly heterogeneous composition tumor cells and their interactions with micro-environment (TME), contribute substantially to poor response treatment high levels morbidity mortality. Here, we used high-dimensional, multiplexed immunohistochemistry map single-cell tissue architecture 26 pediatric samples covering 8 histologic diagnoses, allowing us determine spatial distribution various tumoral subtypes how...
Abstract Glioblastoma (GBM) remains a highly malignant, intrinsically resistant and inevitably recurring brain tumor with dismal prognosis. The aggressiveness lack of effective GBM treatments can be attributed to the heterogeneous plastic nature cells, which easily confer resistance standard-of-care (SOC) therapy. While progression has also been interactions microenvironment, quantitative data describing these are still largely missing. Here, we used high-dimensional, multiplexed...
Abstract High-grade glioma are the main cause of cancer-related death in children. Despite extensive research, their prognosis remains poor with very few treatment options. This can be attributed to highly heterogeneous and plastic nature tumor cells interactions microenvironment, although quantitative data still largely missing. Here, we used high-dimensional, multiplexed immunohistochemistry map spatial, single-cell tissue architecture 31 pediatric samples covering 9 histologic diagnoses....