A5063282588- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Advanced Biosensing Techniques and Applications
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Prostate Cancer Treatment and Research
- Breast Cancer Treatment Studies
- Endometrial and Cervical Cancer Treatments
- Cancer Cells and Metastasis
- Advanced Radiotherapy Techniques
- Salivary Gland Tumors Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Nanopore and Nanochannel Transport Studies
- Cell Adhesion Molecules Research
- Characterization and Applications of Magnetic Nanoparticles
- Cancer survivorship and care
- Phytoestrogen effects and research
- RNA modifications and cancer
- Metastasis and carcinoma case studies
- BRCA gene mutations in cancer
- Nanofabrication and Lithography Techniques
- Aldose Reductase and Taurine
- Breast Lesions and Carcinomas
- Advanced biosensing and bioanalysis techniques
- Cardiac Ischemia and Reperfusion
Ghent University Hospital
2017-2024
Ghent University
2017-2023
Cancer Research Institute Ghent
2017-2023
Abstract Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation characterization protocols. We assessed the impact some commonly implemented pre-analytical, analytical post-analytical variables in EV research. Centrifugal filters with different membrane types pore sizes are used to reduce large volume biofluids prior or concentrate EVs. compared five reported for their efficiency when using plasma, urine EV-spiked PBS. Regenerated...
Abstract Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity biofluids technical variation introduced during sample preparation analysis. To understand mitigate these limitations, we generated trackable recombinant (rEV) as a reference material. Employing complementary characterization methods, demonstrate that rEV are stable bear...
ABSTRACT Extracellular vesicles (EV) are increasingly being recognized as important vehicles of intercellular communication and promising diagnostic prognostic biomarkers in cancer. Despite this enormous clinical potential, the plethora methods to separate EV from biofluids, providing material highly variable purity, lacking knowledge regarding methodological repeatability pose a barrier translation. Urine is considered an ideal proximal fluid for study urological cancers due its direct...
Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding biomarker development. In this study, we fractionate by combining size-exclusion chromatography (SEC) OptiPrep density gradient centrifugation to study clinical context-dependent time-dependent variations in the biomolecular landscape of systemically circulating EV. Using pooled samples breast cancer patients, first demonstrate technical repeatability fractionation. serial...
Abstract The analysis of extracellular vesicles (EV) in blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health disease. Technical variation must be minimized confidently assess EV‐associated biomarkers, but impact pre‐analytics on EV characteristics remains minimally explored. We present results from first large‐scale Blood Benchmarking (EVBB) study which we systematically compared 11 collection tubes (BCT; six preservation...
The nodal burden of patients with residual isolated tumor cells (ITCs) in the sentinel lymph nodes (SLNs) after neoadjuvant chemotherapy (NAC) (ypN0i+) is unknown, and axillary management not standardized. We investigated rates additional positive (LNs) at node dissection (ALND) oncologic outcomes ypN0i+ treated without ALND.
Breast cancer cells closely interact with different cell types of the surrounding adipose tissue to favor invasive growth and metastasis. Extracellular vesicles (EVs) are nanometer-sized secreted by that shuttle proteins nucleic acids establish cell-cell communication. To study role EVs released cancer-associated in breast progression metastasis a standardized EV isolation protocol obtains pure maintains their functional characteristics is required. We implemented differential...
In the surgical treatment of breast cancer, primary tumor is removed together with sentinel lymph nodes (SLNs). Current clinical procedures involve radioactive compounds (exposing both patient and surgeon) blue dyes, which often induce long-lasting skin discoloration. Here, we demonstrate in vivo detection SLNs mice using X-ray computed tomography (CT) near-infrared fluorescence via a dual-modality imaging probe. CT contrast provided by hafnium oxide nanocrystals, stabilized physiological...
Extracellular vesicles (EV) are extensively studied in human body fluids as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the specificity and reproducibility EV sample preparation well intensive manual labor. We present an automated liquid handling workstation density-based separation from compare its performance to by (in)experienced researchers.Automated versus trackable recombinant extracellular (rEV) spiked PBS significantly reduces...
Background: The prognostic and predictive role of stromal tumor-infiltrating lymphocytes (sTILs) is undetermined in pleomorphic invasive lobular cancer (pILC). same applies for the expression PD-1/PD-L1 this rare breast subtype. Here, we aimed to investigate sTILs, analyze PD-L1 levels pILC.
 Methods: Archival tissues from sixty-six patients with pILC were collected. sTIL density was scored as a percentage tumor area, using following cut-offs: 0%; <5%; 5-9%; 10-50%. analyzed IHC...
Neo-adjuvant radiotherapy (NART) for breast cancer has shown promising survival results in retrospective trials. However, there are some obstacles such as a chemotherapy delay, an increased overall treatment time (OTT) and the risk of increasing surgical morbidity. Accelerated (RT) 5 fractions allows to deliver NART very short span minimizes delay surgery chemotherapy. This trial investigates this schedule safety, feasibility OTT.Twenty patients eligible neo-adjuvant (NACT) conserving...
Introduction Breast cancer is the most common type in women worldwide. Due to hormone receptor positivity majority of breast tumours endocrine therapy a crucial part treatment landscape cancer. Endocrine consists use selective oestrogen-receptor modulators or aromatase inhibitors. These medicines generate hypoestrogenic environment by reducing circulating oestrogen altering effect on tissue cells blockade. As side effect, vulvovaginal atrophy occurs patients with using therapy. Vulvovaginal...
Background: The prognostic and predictive role of stromal tumor-infiltrating lymphocytes (sTILs) is undetermined in pleomorphic invasive lobular cancer (pILC). same applies for the expression PD-1/PD-L1 this rare breast subtype. Here, we aimed to investigate sTILs analyze PD-L1 levels pILC. Methods: Archival tissues from sixty-six patients with pILC were collected. sTIL density was scored as a percentage tumor area using following cut-offs: 0%; <5%; 5–9%; 10–50%. analyzed IHC on...
<h3>Introduction/Background</h3> Vulvar cancer is a rare malignancy. Treatment in the early stages consists of broad excision and sentinel node procedure. Tumour remains challenging due to high risk local recurrence (43%) associated with positive tumour margins. Therefore, precise method preserve healthy tissue detect margins intra-operatively, needed. Direct intra-operative assessment would avoid need for re-operation their psychological, technical, financial consequences. Currently, margin...
Abstract Background: Breast cancer is the most common type in women worldwide. Due to hormone receptor positivity majority of breast tumors endocrine therapy a crucial part treatment landscape cancer. Selective estrogen-receptor modulators or aromatase inhibitors are used options for therapy. These medicines generate hypoestrogenic environment by reducing circulating estrogen altering effect on tissue cells blockade. As side effect, vulvovaginal atrophy occurs patients using Vulvovaginal has...