Hetty Helsmoortel
A5075931930- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Extracellular vesicles in disease
- Acute Myeloid Leukemia Research
- Neuroblastoma Research and Treatments
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Signaling Pathways in Disease
- Acute Lymphoblastic Leukemia research
- Lung Cancer Research Studies
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Cancer therapeutics and mechanisms
- interferon and immune responses
- Digital Imaging for Blood Diseases
- Childhood Cancer Survivors' Quality of Life
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Sarcoma Diagnosis and Treatment
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Blood disorders and treatments
Cancer Research Institute Ghent
2017-2024
Ghent University
2018-2024
Ghent University Hospital
2014-2024
Abstract RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, sequencing methods quantify total content outside cells are rare. Here, we evaluate performance SMARTer Stranded Total RNA-Seq method platelet-rich plasma, platelet-free urine, conditioned medium, and vesicles (EVs) from these We found be accurate, precise, compatible with low-input volumes able few thousand genes....
Overwhelming evidence indicates that long non-coding RNAs have essential roles in tumorigenesis. Nevertheless, their role the molecular pathogenesis of pediatric B-cell precursor acute lymphoblastic leukemia has not been extensively explored. Here, we conducted a comprehensive analysis RNA transcriptome ETV6/RUNX1-positive BCP-ALL, one most frequent subtypes leukemia. First, used primary patient samples to identify an ETV6/RUNX1 specific expression signature consisting 596 lncRNA...
In this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of noninvasive biomarkers with potential in monitoring metastatic disease. After determining miRNome, 743 miRNAs were screened 2 independent cohorts 131 and 54 patients. Evaluation miRNA variance a model testing tumor stage, MYCN status, age at diagnosis, overall survival revealed stage as most significant factor impacting abundance serum. Differential analysis between patients...
Extracellular RNA (cell-free RNA; exRNA) from blood-derived liquid biopsies is an appealing, minimally invasive source of disease biomarkers. As pre-analytical variables strongly influence exRNA measurements, their reporting essential for meaningful interpretation and replication results. The aim this review was to chart what extent are documented, pinpoint shortcomings improve future reporting. In total, 200 blood plasma studies published in 2018 or 2023 were reviewed annotation 22...
DNA copy number analysis has been instrumental for the identification of genetic alterations in B-cell precursor acute lymphoblastic leukemia. Notably, some these defects have associated with poor treatment outcome and might be relevant future risk stratification. In this study, we characterized recurrent deletions CD200 BTLA genes, mediated by recombination-activating used breakpoint-specific polymerase chain reaction assay to screen a cohort 1154 cases leukemia uniformly treated according...
<title>Abstract</title> Using blood-based extracellular RNA (circulating cell-free RNA; exRNA) as a clinical biomarker requires validated procedures for sample collection and processing quantification. No study to date has systematically tested pre-analytical variables affecting transcriptome-wide exRNA analysis. We evaluated compared ten blood tubes, three time intervals between draw processing, eight purification methods using the supplier-specified minimal maximal biofluid input volumes....
Abstract The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum biofluid input...
Abstract RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, sequencing methods quantify total content outside cells are rare. Here, we evaluate performance SMARTer Stranded Total RNA-Seq method platelet-rich plasma, platelet-free urine, conditioned medium, and vesicles (EVs) from these We found be accurate, precise, compatible with low-input volumes able few thousand genes....
<title>Abstract</title> The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum...
Abstract In this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of non-invasive biomarkers with potential in monitoring metastatic disease. After determining miRNome, 743 miRNAs were screened two independent cohorts 131 and 54 patients. Evaluation miRNA variance a model testing tumor stage, MYCN status, age at diagnosis overall survival, revealed stage as most significant factor impacting abundance serum. Differential expression analysis...
Abstract Cancer biomarker studies require procedures that provide accurate and precise test results with high analytical sensitivity. Consequently, the growing use of extracellular RNA from human biofluids as clinically relevant requires implementation benchmarked methods for sample collection, processing, profiling. While several small-scale have pointed at impact individual preanalytical factors, no comprehensive study has addressed many variables affecting downstream sequencing...