A5014202881- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Cancer Genomics and Diagnostics
- Renal and Vascular Pathologies
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Artificial Intelligence in Healthcare
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Extracellular vesicles in disease
- Neutropenia and Cancer Infections
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Foreign Body Medical Cases
- Congenital Heart Disease Studies
- Organ Donation and Transplantation
- Virology and Viral Diseases
- Aortic Disease and Treatment Approaches
- Viral gastroenteritis research and epidemiology
- Multiple Myeloma Research and Treatments
- Eosinophilic Disorders and Syndromes
- Mast cells and histamine
Ghent University Hospital
2015-2024
Cancer Research Institute Ghent
2022-2024
Ghent University
2014-2024
Predictive models for delayed graft function (DGF) after kidney transplantation are usually developed using logistic regression. We want to evaluate the value of machine learning methods in prediction DGF. 497 transplantations from deceased donors at Ghent University Hospital between 2005 and 2011 included. A feature elimination procedure is applied determine optimal number features, resulting 20 selected parameters (24 conversion indicator parameters) out 55 retrospectively collected...
Extracellular RNA (cell-free RNA; exRNA) from blood-derived liquid biopsies is an appealing, minimally invasive source of disease biomarkers. As pre-analytical variables strongly influence exRNA measurements, their reporting essential for meaningful interpretation and replication results. The aim this review was to chart what extent are documented, pinpoint shortcomings improve future reporting. In total, 200 blood plasma studies published in 2018 or 2023 were reviewed annotation 22...
Background:This study compared 22 different definitions of delayed graft function (DGF) following kidney transplantation. Material/Methods:Our included 497 transplantations from deceased donors at our center between 2005 and 2011.Graft survival analysis including log-rank tests Cox proportional hazards model was performed.Sensitivity specificity were calculated in relation to failure. Results:Mean follow-up time 5.1 years.All dialysis-based associated with failure characterized by high...
BACKGROUND:Kidney transplantation is the preferred treatment for patients with end-stage renal disease. Delayed graft function (DGF) a common complication and associated short- long-term outcomes. Several predictive models DGF have been developed. MATERIAL AND METHODS:497 kidney transplantations from deceased donors at our center between 2005–2011 are included. Firstly, accuracy of existing proposed by Irish et al. (M1), Jeldres (M2), Chapal (M3), Zaza (M4) was assessed. Secondly, were...
Abstract Circulating nucleic acids in blood plasma form an attractive resource to study human health and disease. Here, we applied mRNA capture sequencing of cell-free RNA from 266 cancer patients cancer-free controls (discovery n = 208, 25 types; replication 58, 3 types). We observed type-specific as well pan-cancer alterations transcriptomes compared controls. Differentially abundant RNAs were heterogenous among cohorts, hampering identification robust biomarkers. Therefore, developed a...
<title>Abstract</title> Using blood-based extracellular RNA (circulating cell-free RNA; exRNA) as a clinical biomarker requires validated procedures for sample collection and processing quantification. No study to date has systematically tested pre-analytical variables affecting transcriptome-wide exRNA analysis. We evaluated compared ten blood tubes, three time intervals between draw processing, eight purification methods using the supplier-specified minimal maximal biofluid input volumes....
Diffuse large B-cell lymphoma (DLBCL) and primary mediastinal (PMBCL) are aggressive histological subtypes of non-Hodgkin's lymphoma. Improved understanding the underlying molecular pathogenesis has led to new classification risk stratification tools, including development cell-free biomarkers through liquid biopsies. The goal this study was investigate RNA (cfRNA) in DLBCL PMBCL patients. Blood plasma samples (n=168) matched diagnostic formalin-fixed paraffin-embedded (FFPE) tissue (n=69)...
Anisakiasis is an emerging zoonosis caused by the fish parasitic nematode Anisakis infecting gastrointestinal tract.We describe a case of 58-year-old woman diagnosed with gastro-allergic anisakiasis, in which patient developed acute food-induced IgE-mediated hypersensitivity reaction as well concurrent gastro-intestinal manifestations after consumption raw fish. The presented epigastric pain, anaphylaxis and dysphagia eosinophilic oesophagitis.Anisakis allergy should be considered causative...
Abstract The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum biofluid input...
<title>Abstract</title> The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum...
The potential of RNA-based liquid biopsy is increasingly being recognized in diffuse large B-cell lymphoma (DLBCL), the most common subtype non-Hodgkin's lymphoma. This study explores cell-free transcriptome a humanized DLBCL patient-derived tumor xenograft (PDTX) model. Blood plasma samples (n = 171) derived from PDTX model, including 27 (HIS) PDTX, 8 HIS non-PDTX, and 21 non-HIS non-obese diabetic (NOD)-scid IL2Rgnull (NSG) mice were collected during humanization, xenografting, treatment,...