A5008250826- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Immune cells in cancer
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Peripheral Artery Disease Management
- Dermatology and Skin Diseases
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Inflammasome and immune disorders
- Rheumatoid Arthritis Research and Therapies
- Allergic Rhinitis and Sensitization
- Cancer-related molecular mechanisms research
- Autoimmune and Inflammatory Disorders Research
- Pediatric health and respiratory diseases
- Viral-associated cancers and disorders
- Cell Adhesion Molecules Research
- Pharmacological Effects of Natural Compounds
- Histone Deacetylase Inhibitors Research
- Gestational Diabetes Research and Management
- Periodontal Regeneration and Treatments
- Dietary Effects on Health
- Medical and Biological Ozone Research
- Vagus Nerve Stimulation Research
PhysioPathologie des Adaptations Nutritionnelles
2015-2017
University of Ottawa
2016
University of Bristol
2011-2015
University of Sassari
2012-2015
Imperial College London
2015
Mayo Clinic
2015
Lung Institute
2015
NIHR Bristol Cardiovascular Biomedical Research Unit
2014
University of Brescia
2012
Istituti di Ricovero e Cura a Carattere Scientifico
2012
Circulating proangiogenic cells (PACs) support postischemic neovascularization. Cardiovascular disease and diabetes mellitus impair PAC regenerative capacities via molecular mechanisms that are not fully known. We hypothesize a role for microRNAs (miRs). miRs currently investigated as potential diagnostic prognostic biomarkers.
Myocardial infarction (MI) is the leading cause of death worldwide. MicroRNAs regulate expression their target genes, thus mediating a plethora pathophysiological functions. Recently, miRNA-24 emerged as an important but controversial miRNA involved in post-MI responses. Here, we aimed at clarifying effect adenovirus-mediate intra-myocardial delivery decoy for mouse MI model and to investigate impact inhibition on angiogenesis cardiovascular apoptosis. After induction, was lower peri-infarct...
Objective— Gestational diabetes mellitus (GDM) produces fetal hyperglycemia with increased lifelong risks for the exposed offspring of cardiovascular and other diseases. Epigenetic mechanisms induce long-term gene expression changes in response to utero environmental perturbations. Moreover, microRNAs (miRs) control function endothelial cells (ECs) under physiological pathological conditions can target epigenetic machinery. We investigated functional expressional effect GDM on human ECs...
Epigenetic mechanisms may regulate the expression of pro-angiogenic genes, thus affecting reparative angiogenesis in ischemic limbs. The enhancer zest homolog-2 (EZH2) induces thtrimethylation lysine 27 on histone H3 (H3K27me3), which represses gene transcription. We explored (i) if EZH2 is regulated by hypoxia and ischemia; (ii) impact two genes: eNOS BDNF; (iii) functional effect inhibition cultured endothelial cells (ECs); (iv) therapeutic potential a mouse model limb ischemia (LI). was...
Micro-immunotherapy (MI) is a therapeutic option employing low doses (LD) and ultra-low (ULD) of cytokines immune factors to help the organism at modulating responses. In an overpowering inflammatory context, this strategy may support restoration body's homeostasis, as active ingredients MI medicines' (MIM) could boost or slow down physiological functions cells. The aim study evaluate for first time in vitro anti-inflammatory properties some actives employed by MIM interest several human cell models.
Background and Aims Human breast milk is an extremely dynamic fluid containing many biologically-active components which change throughout the feeding period day. We designed a miRNA assay on minimized amounts of raw obtained from mothers preterm infants. investigated changes in expression within month 2 lactation then over course 24 hours. Materials Methods Analyses were performed pooled milk, made by combining samples collected at different clock times same mother donor, along with time...
Tumor-associated macrophages (TAMs) play a pivotal role in cancer regulation by influencing tumor growth, metastasis, and the immune microenvironment. By providing low doses ultra-low (ULD) of regulators to organism, micro-immunotherapy (MI) medicines (MIM) could be seen as valuable adjuvant drugs context wide range pathological conditions, including cancers. Thus, these MIM target TAMs, affecting their phenotype activities. In this study, anti-tumor immune-stimulatory effects four capsules...
Diabetes is a cause of cardiac dysfunction, reduced myocardial perfusion, and ultimately heart failure. Nerve growth factor (NGF) exerts protective effects on the cardiovascular system. This study investigated whether NGF gene transfer can prevent diabetic cardiomyopathy in mice. We worked with mice streptozotocin-induced type 1 diabetes nondiabetic control After having established that reduces mRNA expression, we tested therapies adeno-associated viral vectors (AAVs) for capacity to protect...
Tumor necrosis factor-α (TNF-α) and IL-1β are 2 pro-inflammatory cytokines known to be involved in rheumatic diseases. The therapeutic strategy used micro-immunotherapy (MI) reduce chronic inflammation attenuate pain consists mainly targeting these cytokines. 2LARTH® is a sublingually administered medicine consisting of lactose-saccharose globules impregnated with ethanolic preparations immune mediators nucleic acids at ultra-low doses.The aim the study explore effect MI on TNF-α secretion...
Background: Epstein–Barr virus (EBV) is often kept silent and asymptomatic; however, its reactivation induces a chronic and/or recurrent infection that associated with numerous diseases, including cancer inflammation-related disorders. As no specific treatment currently available, the immune factors-based micro-immunotherapy (MI) medicine 2LEBV® could be considered valuable therapeutic option to sustain system in EBV reactivation. Methods: The present work aimed investigate, for first time,...
Varicella-zoster virus (VZV) affects over 90% of the global population. The initial encounter with VZV, often in early years childhood, results varicella. From latency, VZV can reactivate later stages life, leading to development herpes zoster. Considering importance host immune responses preventing reactivation and clinical manifestations associated infection, a therapy that sustains system could be great interest. present work aimed set basis possible mode action 2LZONA®,...
Mitochondria play a crucial role in oxidative stress control and reactive oxygen species (ROS) generation, impacting many cellular processes. Dysregulated mitochondria are linked to diseases such as colorectal cancer (CRC), known for its aggressiveness. Since ROS plays tumor growth metastasis, there is considerable interest developing therapies that target these reactives. This study investigates the effects of some active substances from micro-immunotherapy (MI) medicine 2LMIREG® on...
Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, which can cause cartilage and bone damages as well pain disability. In order to prevent disease progression, reduce pain, major symptoms of RA, one good strategy consists in targeting proinflammatory cytokines that have the key role vicious circle synovial inflammation pain. The micro-immunotherapy medicine (MIM) 2LARTH® targets involved inflammation.The aim study evaluate effect MIM compared vehicle an vivo model induced...
Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are pro-inflammatory cytokines involved in acute chronic inflammatory diseases. Indeed, immunotherapy blocking these 2 has been developed. Micro-immunotherapy (MI) also uses ultra-low doses (ULD) of cytokines, impregnated on lactose-sucrose pillules, to counteract their overexpression. The study conducted with objectives: examine the anti-inflammatory effect vitro capacity unitary medicines, TNF-α (27 CH) IL-1β CH), reduce secretion...
As a cytokine, gamma-interferon (IFN-γ) is considered key player in the fine-tuned orchestration of immune responses. The extreme cellular sensitivity to cytokines attested by fact that very few these bioactive molecules per cell are enough trigger functions. These findings can, at least partially, explain how/why homeopathically-prepared cytokines, and especially micro-immunotherapy (MI) medicines, able drive We focused our fundamental research on unitary MI preparation IFN-γ, specifically...
In this study, we evaluated the efficacy of a micro-immunotherapy medicine (MIM), 2LALERG, in preclinical model allergic respiratory disease sensitized with birch pollen extract (BPE). BALB/c mice were immunized BPE, or saline solution, and then challenged. Micro-immunotherapy pillules diluted water, 3 doses (0.75; 1.5; mg/mouse) tested compared to vehicle control (3 mg/mouse). Treatments orally administered by gavage for 10 days. (0.75 reduced number total cells as well levels interleukin...
In this study, the immunomodulatory effects of a sequential micro-immunotherapy medicine, referred as MIM-seq, were appraised in human primary M1 and M2 macrophages, which secretion pro-inflammatory cytokines, such interleukin (IL)-1β, IL-6, IL-12, IL-23, tumor necrosis factor (TNF)-alpha, was inhibited. addition, potential anti-proliferative MIM-seq on cells assessed three models colorectal cancer (CRC): an vitro two-dimensions (2D) model HCT-116 cells, tri-dimensional (3D) spheroids, vivo...
As one of the major cytokines implicated in orchestration immune responses, interleukin 6 (IL-6) can either act as a pro- or an anti-inflammatory factor, depending on micro-environment. In micro-immunotherapy (MI) medicines, IL-6 is employed at low doses (LD) and ultra-low (ULD), expressed centesimal Hahnemannian (CH), used alone combination with other regulators to modulate patients’ responses. The present study focused assessing vitro immune-modulatory effects two IL-6-containing MI...
This study aimed at evaluating the effects of micro-immunotherapy medicine (MIM) 2LEID, both in vitro and vivo, on several components innate adaptive immune system. MIM increased phagocytic activity macrophages, it augmented expression activation markers CD69 HLA-DR NK cells monocytes/macrophages, respectively. The effect was evaluated a model respiratory infection induced by influenza A virus administration to immunocompetent mice which able improve neutrophil recruitment within lungs (p =...
Periodontal therapies use immune mediators, but their side effects can increase with dosage. Micro-immunotherapy (MI) is a promising alternative that employs regulators at low and ultralow doses to minimize adverse effects. In this study, the of 5 capsules entire 10-capsule sequence sequential MI medicine (MIM-seq) were tested in two vitro models periodontitis. Firstly, human gingival fibroblasts (hGFs) exposed interleukin (IL)-1β induce inflammation treated five different MIM-seq for 3 days...
Human papillomavirus (HPV) is the second most common infectious agent causing cancer. Persistent infection with high-risk (HR)-HPV can lead to cervical intra-epithelial neoplasia and carcinomas (CC). While host immune response necessary for viral clearance, chronic activation contributes a low-grade inflammation that ultimately carcinogenesis. The micro-immunotherapy medicine (MIM) 2LPAPI® could be valuable tool manage clearance of virus reduce risk developing CC. In this in vitro study, we...