A5091425823- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- Clusterin in disease pathology
- RNA modifications and cancer
- Nerve injury and regeneration
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Cancer, Lipids, and Metabolism
- Biomarkers in Disease Mechanisms
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Coronary Interventions and Diagnostics
- RNA Research and Splicing
- Mesenchymal stem cell research
- Autophagy in Disease and Therapy
- Peripheral Artery Disease Management
- Genomics and Chromatin Dynamics
- Telomeres, Telomerase, and Senescence
- Wound Healing and Treatments
- Epigenetics and DNA Methylation
- IL-33, ST2, and ILC Pathways
- Cardiac Ischemia and Reperfusion
- RNA Interference and Gene Delivery
Università di Camerino
2025
University of Teramo
2025
British Heart Foundation
2011-2024
University of Edinburgh
2015-2024
The Queen's Medical Research Institute
2014-2024
Queen's Medical Centre
2020-2024
University of Bristol
2008-2020
NIHR Bristol Cardiovascular Biomedical Research Unit
2008-2019
British Heart Foundation Centre for Cardiovascular Science
2014
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2012
Background— Diabetes mellitus impairs endothelial cell (EC) function and postischemic reparative neovascularization by molecular mechanisms that are not fully understood. microRNAs negatively regulate the expression of target genes mainly interaction in their 3′ untranslated region. Methods Results— We found microRNA-503 (miR-503) ECs is upregulated culture conditions mimicking diabetes (high D-glucose) ischemia-associated starvation (low growth factors). Under normal conditions,...
High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand libraries can access far greater space, provided that the predictive accuracy sufficient useful Through largest and most diverse virtual HTS campaign reported date, comprising 318 individual projects, we demonstrate our AtomNet® convolutional neural network...
Objective— The impact of diabetes on the bone marrow (BM) microenvironment was not adequately explored. We investigated whether induces microvascular remodeling with negative consequence for BM homeostasis. Methods and Results— found profound structural alterations in from mice type 1 depletion hematopoietic component fatty degeneration. Blood flow (fluorescent microspheres) density (immunohistochemistry) were remarkably reduced. Flow cytometry verified MECA-32 + endothelial cells. Cultured...
Circulating proangiogenic cells (PACs) support postischemic neovascularization. Cardiovascular disease and diabetes mellitus impair PAC regenerative capacities via molecular mechanisms that are not fully known. We hypothesize a role for microRNAs (miRs). miRs currently investigated as potential diagnostic prognostic biomarkers.
Nerve growth factor (NGF) promotes angiogenesis and cardiomyocyte survival, which are both desirable for postinfarction myocardial healing. Nonetheless, the NGF potential cardiac repair has never been investigated.To define expression localization of its high-affinity receptor TrkA (tropomyosin-related A) in human infarcted heart to investigate roles endogenous engineered using a mouse model infarction (MI).Immunostaining was performed on samples from humans deceased MI or unrelated...
Myocardial infarction (MI) is the leading cause of death worldwide. MicroRNAs regulate expression their target genes, thus mediating a plethora pathophysiological functions. Recently, miRNA-24 emerged as an important but controversial miRNA involved in post-MI responses. Here, we aimed at clarifying effect adenovirus-mediate intra-myocardial delivery decoy for mouse MI model and to investigate impact inhibition on angiogenesis cardiovascular apoptosis. After induction, was lower peri-infarct...
In response to blood vessel wall injury, aberrant proliferation of vascular smooth muscle cells (SMCs) causes pathological remodeling. However, the controlling mechanisms are not completely understood.
Abstract Our objective was to identify microRNA (miRNA) biomarkers of drug-induced liver and kidney injury by profiling the circulating miRNome in patients with acetaminophen overdose. Plasma miRNAs were quantified age- sex-matched overdose (N = 27) without organ (APAP-TOX APAP-no TOX, respectively). Classifier tested a separate cohort 81). miRNA specificity determined non-acetaminophen murine models. Sensitivity stratification at hospital presentation 67). From 1809 miRNAs, 75 3-fold or...
Studies in transgenic mice showed the key role of (Pim-1) (proviral integration site for Moloney murine leukemia virus-1) control cardiomyocyte function and viability.We investigated whether Pim-1 represents a novel mechanistic target cure diabetic cardiomyopathy, steadily increasing cause nonischemic heart failure.In streptozotocin-induced type 1 mice, protein levels declined during progression along with upregulation inhibitors, phosphatase 2A, microRNA-1. Moreover, hearts low...
Abstract The communication between vascular endothelial cells (ECs) and pericytes in the microvasculature is fundamental for growth homeostasis; however, these processes are disrupted by diabetes. Here we show that modulation of p75 NTR expression ECs exposed to high glucose activates transcription miR-503, which negatively affects pericyte function. NF-κB bind miR-503 promoter upregulate ECs. further induces activation Rho kinase shedding microparticles carrying transfer from pericytes....
Clusterin (CLU) protein is widely distributed in animal tissues and involved many different processes, including apoptosis neoplastic transformation. Green tea catechins (GTC) are known to exert chemopreventive effects cancer models, transgenic adenocarcinoma mouse prostate (TRAMP) mice that spontaneously develop (CaP). We report here growth of SV40-immortalized human epithelial cells (PNT1A) as well tumorigenic, poorly differentiated (PC-3) was potently inhibited by EGCG, the major green...
The increasing incidence of diabetes mellitus will result in a new epidemic heart failure unless novel treatments able to halt diabetic cardiomyopathy early its course are introduced. This study aimed determine whether the activity Akt/Pim-1 signaling pathway is altered at critical stages and supplementation with vitamin B1 analog benfotiamine (BFT) helps sustain above prosurvival mechanism, thereby preserving cardiomyocyte viability function.Untreated streptozotocin-induced type 1 or...
MicroRNAs (miRNAs) directly regulate gene expression at a post-transcriptional level and represent an attractive therapeutic target for wide range of diseases. Here, we report novel strategy delivering miRNAs to endothelial cells (ECs) angiogenesis, using polymer functionalized carbon nanotubes (CNTs). CNTs were coated with two different polymers, polyethyleneimine (PEI) or polyamidoamine dendrimer (PAMAM), followed by conjugation miR-503 oligonucleotides as recognized regulators...
Transforming growth factor beta (TGF-β) is crucial for regulation of the endothelial cell (EC) homeostasis. Perturbation TGF-β signaling leads to pathological conditions in vasculature, causing cardiovascular disease and fibrotic disorders. The pathway critical endothelial-to-mesenchymal transition (EndMT), but a gap remains our understanding related endothelium. This study applied gain- loss-of function approach an vivo model skin wound healing demonstrate that miR-148b regulates has key...