A5040705718- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Retinal Development and Disorders
- DNA and Nucleic Acid Chemistry
- Retinal and Macular Surgery
- Virus-based gene therapy research
- Intraperitoneal and Appendiceal Malignancies
- Retinal Diseases and Treatments
- Advanced Fluorescence Microscopy Techniques
- Inhalation and Respiratory Drug Delivery
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Supramolecular Self-Assembly in Materials
- Retinal and Optic Conditions
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Lipid Membrane Structure and Behavior
- Protein Interaction Studies and Fluorescence Analysis
- Corneal Surgery and Treatments
- Advanced Drug Delivery Systems
- RNA regulation and disease
- Vascular Malformations Diagnosis and Treatment
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
Ghent University
2016-2025
Cancer Research Institute Ghent
2016-2025
Ghent University Hospital
2019-2020
Pharmaceutical Biotechnology (Czechia)
2020
University College London
2016
In gene therapy, endosomal escape represents a major bottleneck since nanoparticles often remain entrapped inside endosomes and are trafficked toward the lysosomes for degradation. A detailed understanding of barrier would be beneficial developing rational strategies to improve transfection escape. By visualizing individual events in live cells, we obtain insight into mechanistic factors that influence proton sponge-based comparative study, found HeLa cells treated with JetPEI/pDNA...
Drug delivery to the posterior segment of eye is challenging due several anatomical and physiological barriers. Thus, there a need for prolonged action targeted drug treat retinal diseases. Intravitreal injections avoid anterior barriers, but vitreoretinal interface inner limiting membrane (ILM) may prevent access systems retina. Existing data on permeation intravitreal nanoparticles are sparse probably misleading inter-species differences structures in rodents humans. To bridge this gap,...
Messenger RNA (mRNA) is revolutionizing the future of therapeutics in a variety diseases, including neurological disorders. Lipid formulations have shown to be an effective platform technology for mRNA delivery and are basis approved vaccines. In many these lipid formulations, polyethylene glycol (PEG)-functionalized provides steric stabilization thus plays key role improving stability both ex vivo vivo. However, immune responses towards PEGylated lipids may compromise use those some...
To gain a better understanding of intracellular processing nanomedicines, we employed quantitative live-cell fluorescence colocalization microscopy to study endosomal trafficking polyplexes in retinal pigment epithelium cells. A new, dynamic algorithm was developed, based on particle tracking and trajectory correlation, allowing for spatiotemporal characterization internalized comparison with compartments labeled EGFP constructs. This revealed early the specifically Rab5-...
Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in intact vitreous body. Materials & methods: Excised bovine eyes were prepared preserve fragile structure humor, while permitting high-resolution fluorescence microscopy single-particle tracking analysis intravitreally injected nanoparticles. This was validated by analyzing polystyrene beads further employed gene nanomedicines composed poly(amido amine)s plasmid DNA. Results: The able...
Small nucleic acid (NA) therapeutics, such as small interfering RNA (siRNA), are generally formulated in nanoparticles (NPs) to overcome the multiple extra- and intracellular barriers upon vivo administration. Interaction with target cells typically triggers endocytosis sequesters NPs endosomes, thus hampering pharmacological activity of encapsulated siRNAs that occurs cytosol. Unfortunately, for most state-of-the-art NPs, endosomal escape is largely inefficient. As a result, bulk...