A5054193594- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Parasites and Host Interactions
- Immune cells in cancer
- Macrophage Migration Inhibitory Factor
- Galectins and Cancer Biology
- Insect symbiosis and bacterial influences
- Complement system in diseases
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Radiopharmaceutical Chemistry and Applications
- Toxin Mechanisms and Immunotoxins
- Cancer Research and Treatments
- Psoriasis: Treatment and Pathogenesis
- Aquaculture disease management and microbiota
- Phagocytosis and Immune Regulation
- Biochemical and Structural Characterization
- Invertebrate Immune Response Mechanisms
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Peptidase Inhibition and Analysis
- Bacterial biofilms and quorum sensing
VIB-UGent Center for Inflammation Research
2016-2025
Vrije Universiteit Brussel
2016-2025
Vlaams Instituut voor Biotechnologie
2004-2022
VIB-VUB Laboratory Myeloid Cell Immunology
2012-2020
Universitätsklinikum Würzburg
2009
Hôpital Fontmaure
2005
University of Würzburg
2004
Emory University Hospital
1997
Baxter (United States)
1997
Baylor University Medical Center
1997
Tumors contain a heterogeneous myeloid fraction comprised of discrete MHC-II(hi) and MHC-II(lo) tumor-associated macrophage (TAM) subpopulations that originate from Ly6C(hi) monocytes. However, the mechanisms regulating abundance phenotype distinct TAM subsets remain unknown. Here, we investigated role colony-stimulating factor (M-CSF) in differentiation polarization different mouse tumor models. We demonstrate treatment tumor-bearing mice with blocking anti-M-CSFR monoclonal antibody...
Nanobodies (Nbs), the variable domains of camelid heavy chain-only antibodies, are a promising class therapeutics or in vivo imaging reagents entering clinic. They possess unique characteristics, including minimal size, providing fast pharmacokinetics, high-target specificity, and an affinity (sub-)nanomolar range conjunction with easy selection production, which allow them to outperform conventional antibodies for radiotherapeutic purposes. As all protein theranostics, extended safety...
Antigen variation is a successful defense system adopted by several infectious agents to evade the host immune response. The principle of this strategy in African trypanosome paradigm involves dense packing variant surface glycoproteins (VSG) exposing only highly variable and immuno-dominant epitopes system, whereas conserved become inaccessible for large molecules. Reducing size binders that target conserved, less-immunogenic, cryptic VSG forms an obvious solution combat these parasites....
The vitamin D receptor (VDR) is a hormone nuclear regulating bone and calcium homeostasis. Studies revealing the expression of VDR on immune cells point toward role for VDR-dependent signaling pathways in immunity. Here we verified ability natural ligand, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to interfere inflammatory T cell stimulatory capacity macrophages, particular within chronic disease features experimental type 1 diabetes (T1D). We demonstrated that constitutively expressed...
Liver X receptors (LXRs) are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. However, exactly how LXRs modulate inflammation during infection remains unknown. To explore this, we used a mouse model Mycobacterium tuberculosis infection. Upon intratracheal with M. tuberculosis, LXR target genes were induced CD11c+ lung alveolar cells. Furthermore, mice deficient both isoforms, LXRα LXRβ (Lxra–/–Lxrb–/– mice), more...
Macrophages are often prominently present in the tumor microenvironment, where distinct macrophage populations can differentially affect progression. Although metabolism influences function, studies on metabolic characteristics of ex vivo tumor-associated (TAM) subsets rather limited. Using transcriptomic and analyses, we now reveal that pro-inflammatory major histocompatibility complex (MHC)-IIhi TAMs display a hampered tricarboxylic acid (TCA) cycle, while reparative MHC-IIlo show higher...
The TNF-alpha-inducing capacity of different trypanosome components was analyzed in vitro, using as indicator cells a macrophage cell line (2C11/12) or peritoneal exudate from LPS-resistant C3H/HeJ mice and LPS-sensitive C3H/HeN mice. variant-specific surface glycoprotein (VSG) identified the major component present trypanosome-soluble extracts. Both soluble (sVSG) membrane-bound VSG (mfVSG) were shown to manifest similar capacities, indicating that dimyristoylglycerol (DMG) compound mfVSG...
Abstract The initial host response toward the extracellular parasite Trypanosoma brucei is characterized by early release of inflammatory mediators associated with a type 1 immune response. In this study, we show that dependent on activation innate system mediated adaptor molecule MyD88. present MyD88-deficient macrophages are nonresponsive both soluble variant-specific surface glycoprotein (VSG), as well membrane-bound VSG purified from T. brucei. Infection mice either clonal or nonclonal...
The development of classically activated monocytic cells (M1) is a prerequisite for effective elimination parasites, including African trypanosomes. However, persistent activation M1 that produce pathogenic molecules such as TNF and NO contributes to the trypanosome infection-associated tissue injury liver cell necrosis in experimental mouse models. Aiming identify mechanisms involved regulation activity, we have recently documented during Trypanosoma brucei infection, CD11b+Ly6C+CD11c+ iNOS...
Scientists found a new miRNA involved in macrophage responses, paving the way for an improved management of inflammatory diseases.
Abstract Background Sodalis glossinidius , a gram-negative bacterial endosymbiont of the tsetse fly, has been proposed as potential in vivo drug delivery vehicle to control trypanosome parasite development an approach known paratransgenesis. Despite this interest S. paratransgenic platform organism flies, few effector molecules have identified so far and date none these successfully expressed bacterium. Results In study, was transformed express single domain antibody, (Nanobody ® ) Nb_An33,...
ABSTRACT The increased use of medical implants has resulted in a concomitant rise device-related infections. majority these infections are caused by Staphylococcus epidermidis biofilms. Immunoprophylaxis and immunotherapy targeting vivo -expressed, biofilm-associated, bacterial cell surface-exposed proteins promising new approaches to prevent treat biofilm-related infections, respectively. Using an silico procedure, we identified 64 that predicted be S . e pidermidis s urface exposed (Ses),...
The African trypanosome Trypanosoma brucei, which persists within the bloodstream of mammalian host, has evolved potent mechanisms for immune evasion. Specifically, antigenic variation variant-specific surface glycoprotein (VSG) and a highly active endocytosis recycling coat efficiently delay killing mediated by anti-VSG antibodies. Consequently, conventional VSG-specific intact immunoglobulins are non-trypanocidal in absence complement. In sharp contrast, monovalent antigen-binding...
To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices dairy cows towards mastitis-causing pathogens.Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven these strains against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). chromogenes L217 the strongest inhibitory effect, being active all tested pathogens most CoNS. Based on cation exchange...
Background In order to promote infection, the blood-borne parasite Trypanosoma brucei releases factors that upregulate arginase expression and activity in myeloid cells. Methodology/Principal findings By screening a cDNA library of T. with an antibody neutralizing arginase-inducing released factors, we identified Kinesin Heavy Chain isoform, termed TbKHC1, as responsible for this effect. Following interaction mouse cells, natural or recombinant TbKHC1 triggered SIGN-R1 receptor-dependent...
Background and aimsLiver macrophages fulfill various homeostatic functions represent an essential line of defense against pathogenic insults. However, it remains unclear whether a history infectious disease in the liver instructs long-term alterations to macrophage compartment.MethodsWe utilized curable model parasitic infection invoked by protozoan parasite Trypanosoma brucei investigate can durably reshape hepatic identity function. Employing combination fate mapping, single cell...