A5059525483- Liver Disease Diagnosis and Treatment
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Liver Disease and Transplantation
- Diabetes Management and Research
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Immune cells in cancer
- Organ Transplantation Techniques and Outcomes
- Vitamin C and Antioxidants Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Research on Leishmaniasis Studies
- T-cell and B-cell Immunology
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Lipid metabolism and disorders
- Single-cell and spatial transcriptomics
- Cardiovascular Disease and Adiposity
- Macrophage Migration Inhibitory Factor
- Adipokines, Inflammation, and Metabolic Diseases
- Alcohol Consumption and Health Effects
KU Leuven
2015-2024
Universitair Ziekenhuis Leuven
2019
Aarhus University Hospital
2018
Institut Scientifique de Santé Publique
2005-2017
Catholic University of America
2014
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2010-2013
Ludwig Cancer Research
2010
UCLouvain
2010
Ghent University Hospital
2009
Vlaams Instituut voor Biotechnologie
2003-2006
Objective Macrophage interleukin (IL)-10 signalling plays a critical role in the maintenance of regulatory phenotype that prevents development IBD. We have previously found anti-tumour necrosis factor (TNF) monoclonal antibodies act through Fcγ-receptor (FcγR) to promote repolarisation proinflammatory intestinal macrophages CD206+ phenotype. The IL-10 anti-TNF-induced macrophage has not been examined. Design used human peripheral blood monocytes and mouse bone marrow-derived study production...
Current interventions for arresting autoimmune diabetes have yet to strike the balance between sufficient efficacy, minimal side effects, and lack of generalized immunosuppression. Introduction antigen via gut represents an appealing method induction antigen-specific tolerance. Here, we developed a strategy tolerance restoration using mucosal delivery in mice biologically contained Lactococcus lactis genetically modified secrete whole proinsulin autoantigen along with immunomodulatory...
The vitamin D receptor (VDR) is a hormone nuclear regulating bone and calcium homeostasis. Studies revealing the expression of VDR on immune cells point toward role for VDR-dependent signaling pathways in immunity. Here we verified ability natural ligand, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to interfere inflammatory T cell stimulatory capacity macrophages, particular within chronic disease features experimental type 1 diabetes (T1D). We demonstrated that constitutively expressed...
Liver X receptors (LXRs) are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. However, exactly how LXRs modulate inflammation during infection remains unknown. To explore this, we used a mouse model Mycobacterium tuberculosis infection. Upon intratracheal with M. tuberculosis, LXR target genes were induced CD11c+ lung alveolar cells. Furthermore, mice deficient both isoforms, LXRα LXRβ (Lxra–/–Lxrb–/– mice), more...
Growing insight into the pathogenesis of type 1 diabetes (T1D) and numerous studies in preclinical models highlight potential antigen-specific approaches to restore tolerance efficiently safely. Oral administration protein antigens is a preferred method for induction, but degradation during gastrointestinal passage can impede such protein-based therapies, reducing their efficacy making them cost-ineffective. To overcome these limitations, we generated tolerogenic bacterial delivery...
The use of hypocalcemic vitamin D analogs is an appealing strategy to exploit the immunomodulatory actions active in vivo while circumventing its calcemic side effects. functional modulation dendritic cells by these molecules regarded as key mechanism underlying their ability regulate T cell reactivity. In this article, we demonstrate capacity analog, TX527, target directly. Microarray analysis purified human CD3(+) cells, cultured presence revealed differential expression genes involved...
The biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], is able to promote the generation tolerogenic mature dendritic cells (mDCs) with an impaired ability activate autoreactive T cells. These could represent a reliable tool for promotion or restoration Ag-specific tolerance through vaccination strategies, example in type 1 diabetes patients. However, successful transfer 1,25(OH)2D3-treated mDCs (1,25D3-mDCs) depends on capacity 1,25(OH)2D3 imprint similar profile...
ABSTRACT Hypoxia and low concentrations of nitric oxide have been reported to upregulate in vitro gene expression 48 proteins the dormancy (DosR) regulon Mycobacterium tuberculosis . These are thought be essential for survival bacteria during persistence vivo targeted by immune system latent infection humans. Here we analyzed immunogenicity eight DosR regulon-encoded antigens plasmid DNA vaccination BALB/c C57BL/6 mice, i.e., Rv1733c, Rv1738, Rv2029c ( pfkB ), Rv2031c/ hspX acr Rv2032 acg...
Abstract Recognition of conserved pathogen‐associated molecular patterns constitutes a crucial step in the initiation innate immune responses. We studied contribution to host‐pathogen interaction mycolic acid (MA), major lipid component cell envelope macrophage intracellular pathogen Mycobacterium tuberculosis and other mycobacteria. MA administered peritoneal cavity or airways induced unique morphotype, similar foamy derivatives observed tuberculous granulomas characterized by accumulation...
Acute-on-chronic liver failure (ACLF) is associated with dysfunctional circulating monocytes whereby patients become highly susceptible to bacterial infections. Here, we identify the pathways underlying monocyte dysfunction in ACLF and investigate whether metabolic rewiring reinstates their phagocytic inflammatory capacity.Following phenotypic characterisation, performed RNA sequencing on CD14+CD16- from decompensated alcoholic cirrhosis. Additionally, an vitro model mimicking...
Intestinal dysbiosis and elevated lipopolysaccharides (LPS) levels have been implicated in the development of obesity, insulin resistance non-alcoholic steatohepatitis (NASH). In order to determine if LPS are patients with NASH compared fatty liver (NAFL) and, correlated histological severity disease (NAFLD) we LPS, markers bioactivity pro-inflammatory cytokines/chemokines undergoing bariatric surgery. At time surgery a biopsy was taken allowing stratification into well-delineated subgroups...
The introduction of β-cell autoantigens via the gut through Lactococcus lactis (L. lactis) has been demonstrated to be a promising approach for diabetes reversal in NOD mice. Here we show that combination therapy low-dose anti-CD3 with clinical-grade self-containing L. lactis, appropriate human application, secreting proinsulin and interleukin-10, cured 66% mice new-onset diabetes, which is comparable results plasmid-driven lactis. Initial blood glucose concentrations (<350 mg/dL)...
Summary Interleukin‐17A ( IL ‐17A), a pro‐inflammatory cytokine acting on neutrophil recruitment, is known to play an important role during M ycobacterium tuberculosis infection, but the of ‐17 A receptor signalling in immune defence against this intracellular pathogen remains poorly documented. Here we have analysed using C 57 BL /6 mice genetically inactivated subunit RA −/− ). Although early after infection bacterial growth was controlled same extent as wild‐type mice, were defective...
The emergence of regulatory T cells (Tregs) as central mediators peripheral tolerance in the immune system has led to an important area clinical investigation target these for treatment autoimmune diseases such type 1 diabetes. We have demonstrated earlier that vitro from healthy individuals with TX527, a low-calcemic analog bioactive vitamin D, can promote CD4+CD25highCD127low profile and imprint migratory signature specific homing sites inflammation. Towards application D-induced Tregs...
Background & AimsNon-alcoholic fatty liver disease (NAFLD) is reaching epidemic proportions, fueled by the obesity pandemic. In NAFLD, monocytes infiltrate visceral adipose tissue that promote local and hepatic inflammation. It however remains unclear what drives inflammation how immune landscape in differs across NAFLD severity spectrum. We aimed to assimilate macrophage (ATM) heterogeneity a cohort.MethodsVisceral macrophages from lean obese patients stratified into phenotypes underwent...
The GPI-anchored trypanosome variant surface glycoprotein (VSG) triggers macrophages to produce TNF, involved in trypanosomiasis-associated inflammation and the clinical manifestation of sleeping sickness. Aiming at inhibiting immunopathology during experimental Trypanosoma brucei infections, a VSG-derived GPI-based treatment approach was developed. To achieve this, mice were exposed GPI before an infectious challenge. This strategy resulted significant prolonged survival substantial...