A5047485184- Vitamin D Research Studies
- Estrogen and related hormone effects
- Vitamin C and Antioxidants Research
- Biotin and Related Studies
- FOXO transcription factor regulation
- Click Chemistry and Applications
- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- Fluorine in Organic Chemistry
- Cyclopropane Reaction Mechanisms
- Bone health and osteoporosis research
- Endoplasmic Reticulum Stress and Disease
- Diabetes and associated disorders
- Synthesis and Reactivity of Heterocycles
- Bone Metabolism and Diseases
- Adrenal Hormones and Disorders
- Bone health and treatments
- Growth Hormone and Insulin-like Growth Factors
- Immunotherapy and Immune Responses
- Gestational Trophoblastic Disease Studies
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Genomics and Chromatin Dynamics
- Cytokine Signaling Pathways and Interactions
- Synthesis and Reactions of Organic Compounds
KU Leuven
2016-2025
Rutgers, The State University of New Jersey
2002-2015
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2010-2013
AZ Sint-Jan
2012
Ghent University
1996-2007
Universitair Ziekenhuis Leuven
2000
Antwerp University Hospital
1998
Laboratoire de Synthèse Organique
1997
Rega Institute for Medical Research
1989-1995
Metabolic switches in various immune cell subsets enforce phenotype and function. In the present study, we demonstrate that active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), induces human monocyte-derived tolerogenic dendritic cells (DC) by metabolic reprogramming. Microarray analysis demonstrated 1,25(OH)2D3 upregulated several genes directly related to glucose metabolism, tricarboxylic acid cycle (TCA), oxidative phosphorylation (OXPHOS). Although OXPHOS was promoted...
SUMMARY Receptors for 1,25(OH)2vitaminD3 are found in most immune cells and important immunological effects have been described vitro, reflected by its capacity to prevent autoimmunity prolong graft survival. The aim of this study was examine the presence nature enzyme responsible final activation molecule, 1-α-hydroxylase, murine macrophages analyse regulation possible role system. Peritoneal from C57Bl/6 mice were incubated with lipopolysaccharide (LPS; 100 μg/ml), interferon-gamma (IFN-γ;...
Abstract 1α,25‐Dihydroxyvitamin D 3 (1,25(OH) 2 ) has important effects on the growth and function of multiple cell types. These pleiotropic 1,25(OH) are mediated through binding to vitamin receptor (VDR). Several polymorphisms human VDR gene have been identified, with Fok I polymorphism resulting in proteins different structures, a long f ‐VDR or shorter F ‐VDR. The aim this study was investigate functional consequences immune cells. In transfection experiments, presence resulted higher...
The use of hypocalcemic vitamin D analogs is an appealing strategy to exploit the immunomodulatory actions active in vivo while circumventing its calcemic side effects. functional modulation dendritic cells by these molecules regarded as key mechanism underlying their ability regulate T cell reactivity. In this article, we demonstrate capacity analog, TX527, target directly. Microarray analysis purified human CD3(+) cells, cultured presence revealed differential expression genes involved...
The biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], is able to promote the generation tolerogenic mature dendritic cells (mDCs) with an impaired ability activate autoreactive T cells. These could represent a reliable tool for promotion or restoration Ag-specific tolerance through vaccination strategies, example in type 1 diabetes patients. However, successful transfer 1,25(OH)2D3-treated mDCs (1,25D3-mDCs) depends on capacity 1,25(OH)2D3 imprint similar profile...
Abstract Immune cells carry receptors for 1,25-dihydroxyvitamin D3 [1,25(OH)2D3; vitamin D receptor (VDR)] and individuals with severe deficiency have immune abnormalities. The aim of this study was to investigate the role in system by studying VDR-knockout (VDR-KO) mice. VDR-KO mice had same metabolic phenotype as rachitic animals hypocalcemia. Leukocytosis, lymphocyte subset composition different organs, splenocyte proliferation several stimuli were normal, except a lower response anti-CD3...
We previously showed that checkpoint kinase 1 (Chk1) and Claspin, two DNA-damage proteins, were down-regulated by 1,25-dihydroxyvitamin D(3), a known inhibitor of cell proliferation. In the present study, we aimed to investigate transcriptional regulation Chk1 Claspin study their expression levels in human breast cancer tissue. Transient transfection experiments MCF-7 cells promoter activities regulated E2F family transcription factors. Subsequently, transcript Chk1, E2F1 determined...
We correlated serum 25-hydroxyvitamin D 3 (25OHD) levels with tumor characteristics and clinical disease outcome in breast cancer patients assessed the impact of genetic determinants vitamin insufficiency. collected from 1800 early at diagnosis, measured 25OHD by radioimmunoassay (RIA), determined variants D-related genes Sequenom. Multivariable regression models were used to correlate characteristics. Cox proportional hazard assess overall survival (OS), disease-specific (DSS), disease-free...
High homocysteine (HCY) levels are a risk factor for osteoporotic fracture. Furthermore, bone quality and strength compromised by elevated HCY owing to its negative impact on collagen maturation. is cleared cystathionine β-synthase (CBS), the first enzyme in transsulfuration pathway. CBS converts cystathionine, thereby committing it cysteine synthesis. A microarray experiment MC3T3-E1 murine preosteoblasts treated with 1,25-dihydroxyvitamin D(3) [1,25(OH)(2) ] revealed cluster of genes...
Abstract Background Differentiation and maturation of dendritic cells yield a cell type with the ability to prime immune responses towards defence destruction. 1,25(OH) 2 D 3 , active form vitamin fosters development tolerogenic cells. This study aimed evaluate effects on murine behaviour in vitro vivo . Methods Dendritic were differentiated from bone marrow female C57Bl/6 mice presence or absence 10 −8 M for 8 days (IL4 GM‐CSF). Maturation was induced 48 h (IFNγ, LPS BALB/C islet homogenate...
High doses of the active form vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], prevent diabetes in NOD mouse but also elicit unwanted calcemic side effects. Because immune cells themselves can convert into 1,25(OH)2D3 locally, we hypothesized that dietary disease. Thus, evaluated whether administration high regular (800 IU/day) during different periods life (pregnancy and lactation, early [3-14 weeks age], or lifelong [3-35 age]) safely prevents mice. We found only treatment raised serum...