A5075159084- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Bowel Disease
- Immune Cell Function and Interaction
- Microscopic Colitis
- Immune cells in cancer
- Celiac Disease Research and Management
- Autophagy in Disease and Therapy
- Cerebrovascular and Carotid Artery Diseases
- Liver Disease Diagnosis and Treatment
- Cardiovascular Health and Disease Prevention
- Parasites and Host Interactions
- Tuberculosis Research and Epidemiology
- Ferroptosis and cancer prognosis
- Hepatitis B Virus Studies
- Cancer, Hypoxia, and Metabolism
- Hepatitis C virus research
- T-cell and B-cell Immunology
- Biosimilars and Bioanalytical Methods
- Research on Leishmaniasis Studies
- Synthesis and biological activity
- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Helicobacter pylori-related gastroenterology studies
- Retinal Diseases and Treatments
Amsterdam University Medical Centers
2019
Amsterdam UMC Location University of Amsterdam
2010-2018
University of Amsterdam
2010-2018
Objective Macrophage interleukin (IL)-10 signalling plays a critical role in the maintenance of regulatory phenotype that prevents development IBD. We have previously found anti-tumour necrosis factor (TNF) monoclonal antibodies act through Fcγ-receptor (FcγR) to promote repolarisation proinflammatory intestinal macrophages CD206+ phenotype. The IL-10 anti-TNF-induced macrophage has not been examined. Design used human peripheral blood monocytes and mouse bone marrow-derived study production...
We have recently shown that the mode of action IgG1 anti-tumour necrosis factor [TNF] antibodies in inflammatory bowel disease [IBD] requires Fcγ-receptor [FcγR] engagement on macrophages. Here we examine effect signalling by anti-TNF macrophage IL-12/IL-23 secretion.Cytokine production human macrophages was assessed at level RNA and protein. TNF-anti-TNF immune complex formation determined size-exclusion chromatography visualized immunofluorescence. IL-12/IL-23p40 measured CD14+ lamina...
Negative effects on the progression of adenocarcinomas by hyperinsulinaemia and insulin analogue glargine (A21Gly,B31Arg,B32Arg human insulin) have recently been suggested. Most actions this hitherto explained direct stimulation growth potential neoplastic cells its IGF-1 related properties. However, insulin-stimulated angiogenesis could be an additional factor involved in tumour clinical outcomes associated with cancer.Five types adenocarcinoma (breast, colon, pancreas, lung kidney) were...
Anti-tumour necrosis factor [TNF] antibodies induce regulatory macrophages which display a phenotype resembling M2 type macrophages. Anti-TNF induced [Mϕind] have immunosuppressive and wound healing properties. The factors that contribute to the induction of Mϕind remain be explored. Autophagy has been described as is important for function We studied contribution autophagy Mϕind. effect on in vitro using peripheral blood mononuclear cells. Interferon gamma [IFN-γ] [Mφ1] were generated by...
Regulatory macrophages play a critical role in tissue repair, and we have previously shown that anti-tumour necrosis factor [TNF] antibodies induce these vitro vivo IBD patients. The induction of regulatory can be potentiated using the combination anti-TNF thiopurines, consistent with enhanced efficacy this therapy described clinical trials. As thiopurines are unfortunately associated significant side effects, here aimed to identify alternatives for anti-TNF, macrophage model as screening...
Abstract Background Alterations in the mesentery of Crohn’s disease (CD) patients have long been described, although functional importance this tissue is less clear. Some studies hypothesise an anti-inflammatory role for enhanced mesentery, whereas others consider a more pathologic function, given its close proximity to inflamed intestine. Better phenotypic and evaluation cells present warranted improve our understanding disease. We previously shown disproportionate presence macrophages CD...
Background: Treatment with the IgG1 anti-TNF antibodies infliximab and adalimumab achieves complete mucosal healing in a considerable proportion of patients Crohn's disease. In contrast, Fab' fragment certolizumab showed only 4% endoscopic remission. These observations suggest that Fc-region contributes to induction healing. We have previously induces CD206+ regulatory macrophages these were increased lamina propria responders, but not non-responders. Here, we investigate importance Fc-gamma...