A5086705988- Blood Coagulation and Thrombosis Mechanisms
- Hemophilia Treatment and Research
- Monoclonal and Polyclonal Antibodies Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Venomous Animal Envenomation and Studies
- Immunodeficiency and Autoimmune Disorders
- Celiac Disease Research and Management
- Influenza Virus Research Studies
- Blood groups and transfusion
- Intramuscular injections and effects
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Diabetes and associated disorders
- Hemostasis and retained surgical items
- Cardiac and Coronary Surgery Techniques
Janssen (Netherlands)
2017-2023
Johnson & Johnson (Netherlands)
2017
Sanquin
2007-2009
University of Amsterdam
2009
University Medical Center Utrecht
2007
Genmab (Netherlands)
2007
Maastricht University
2007
Hasselt University
2007
Leiden University Medical Center
2002-2004
Loyola University Medical Center
2004
Antibodies play a central role in immunity by forming an interface with the innate immune system and, typically, mediate proinflammatory activity. We describe novel posttranslational modification that leads to anti-inflammatory activity of antibodies immunoglobulin G, isotype 4 (IgG4). IgG4 are dynamic molecules exchange Fab arms swapping heavy chain and attached light (half-molecule) heavy-light pair from another molecule, which results bispecific antibodies. Mutagenesis studies revealed...
Durable influenza protection Vaccines are indispensable for the control and prevention of influenza, but there several challenges to efficacy. Some individuals respond poorly vaccination, virus variation makes targeting optimal antigens difficult. Broadly neutralizing antibodies one solution, they have their own pitfalls, including limited cross-reactivity both A B strains need repeated injections. Now, Laursen et al. developed multidomain with breadth potency. Administered intranasally mice...
The Fc fragment of IgG4 can interact with the another IgG molecule. This interaction is a confounding factor when measuring rheumatoid levels. Recently, we demonstrated that half-molecules exchange to form bispecific Ab. We expected these two phenomena be related and investigated physicochemical aspects Fc-Fc interactions. found >99% monomeric by size-exclusion chromatography; therefore, interactions in fluid phase (if any) would short-lived. However, (125)I-labeled does bind IgG1 coupled...
Activated protein C (APC) exerts its anticoagulant activity via proteolytic degradation of the heavy chains activated factor VIII (FVIIIa) and V (FVa). So far, three APC cleavage sites have been identified in chain FVa: Arg-306, Arg-506, Arg-679. To obtain more insight structural functional implications each individual cleavage, recombinant (rFV) mutants were constructed which two or mutated. After expression COS-1 cells, rFV purified, with thrombin, inactivated by APC. During this study we...
Recently, new missense mutations in the activated protein C (APC) cleavage sites of human factor V (FV) distinct from R506Q (FV Leiden) mutation have been reported. These affect APC site at arginine (Arg) 306 heavy chain FV. Whether these result resistance and are associated with a risk thrombosis is not clear. The main objective present study was to identify APC-resistant phenotype FV molecules different sites. To this, recombinant mutants were reconstituted FV-deficient plasma, after which...
The R2 haplotype of the FV gene spans from exon 8 through 25 and comprises several strongly linked polymorphisms in gene, including some missense mutations. Carriership R2-FV allele has been associated with reduced plasma levels, increased FV1/FV2 ratios mild APC resistance. Some studies have reported that carriership is an risk venous thombosis. At this moment, individual contribution to R2-associated phenotypes different mutations unclear. main objective our study was obtain insight...
Background: COVID-19 vaccines have been widely used to control the SARS-CoV-2 pandemic.In individuals receiving replication-incompetent, adenovirus vector-based or Ad26.COV2.S [Johnson & Johnson-Janssen] vaccines), a very rare but serious adverse reaction has reported and described as vaccine-induced immune thrombotic thrombocytopenia (VITT).The exact mechanism of VITT following vaccination is under investigation.Antibodies directed against human platelet-factor 4 (PF4) are considered...
Thrombocytopenia (VITT).Inflammasome activation is a common feature of Immunothrombosis.Here we hypothesized that inflammasome might be involved in the development VITT.Aims: This study aims to characterize patients with VITT.Methods: Forty post-vaccine thrombosis (PVT) and 39 age/sex matched unvaccinated individuals were included (CAAE-48532621.8.0000.5262and CAAE-52396621.0.0000.5262).Cohort characteristics are described Table-1.Anti-PF4 IgGs (immucor), Tissue Factor (TF), CD62p (...