A5015085896- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Surface Properties and Treatments
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Lignin and Wood Chemistry
- Crop Yield and Soil Fertility
- Enzyme-mediated dye degradation
- Postharvest Quality and Shelf Life Management
- Greenhouse Technology and Climate Control
- Biofuel production and bioconversion
- Environmental Toxicology and Ecotoxicology
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Radioactive contamination and transfer
- Horticultural and Viticultural Research
- Ubiquitin and proteasome pathways
University of Lausanne
2017-2024
Center for Research in Agricultural Genomics
2023-2024
Sainsbury Laboratory
2015-2021
University of Cambridge
2015-2021
University of Helsinki
2012-2015
Helsinki Institute of Physics
2012
Vilnius University
2010
Higher plant function is contingent upon the complex three-dimensional (3D) architecture of tissues, yet severe light scattering renders deep, 3D tissue imaging very problematic. Although efforts to 'clear' tissues have been ongoing for over a century, many innovations made in recent years. Among them, protocol called ClearSee efficiently clears and diminishes chlorophyll autofluorescence while maintaining fluorescent proteins - thereby allowing analysis gene expression protein localisation...
Abstract Plant roots integrate environmental signals with development using exquisite spatiotemporal control. This is apparent in the deposition of suberin, an apoplastic diffusion barrier, which regulates flow water, solutes and gases, environmentally plastic. Suberin considered a hallmark endodermal differentiation but absent tomato endodermis. Instead, suberin present exodermis, cell type that model organism Arabidopsis thaliana . Here we demonstrate regulatory network has same parts...
A powerful method to study gene function is expression or overexpression in an inducible, cell type-specific system followed by observation of consequent phenotypic changes and visualization linked reporters the target tissue. Multiple inducible systems have been developed for plants, but very few these combine plant selection markers, control domains, access multiple promoters protein fusion reporters, chemical induction, high-throughput cloning capabilities. Here, we introduce a MultiSite...
Article18 March 2020Open Access Transparent process SCHENGEN receptor module drives localized ROS production and lignification in plant roots Satoshi Fujita Corresponding Author [email protected] orcid.org/0000-0002-3514-3349 Department of Plant Molecular Biology, Biophore, University Lausanne, Switzerland Search for more papers by this author Damien De Bellis orcid.org/0000-0001-9480-7208 Electron Microscopy Facility, Kai H Edel Institut für Biologie und Biotechnologie der Pflanzen,...
The development and growth of higher plants is highly dependent on the conduction water minerals throughout plant by xylem vessels. In Arabidopsis roots organized as an axis cell files with two distinct fates: central metaxylem peripheral protoxylem. During vascular development, high low expression levels class III HD-ZIP transcription factors promote protoxylem identities, respectively. Protoxylem specification determined both mobile, ground tissue-emanating miRNA165/6 species, which...
Significance Lignin is a defining polymer of vascular plants and great physiological, ecological, economical importance. Yet, its polymerization in the cell wall still not understood. polymerizing enzymes, laccases peroxidases, exist vast numbers plant genomes. By focusing on specific lignin structure, ring-like Casparian strips (CSs), we reduced candidate abolished essentially all with detectable endodermal expression. even slight defects CS formation were detected. contrast, quintuple...
In the plant meristem, tissue-wide maturation gradients are coordinated with specialized cell networks to establish various developmental phases required for indeterminate growth. Here, we used single-cell transcriptomics reconstruct protophloem trajectory from birth of progenitors terminal differentiation in
Abstract Background Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use CRISPR-Cas9 has increased rapidly recent years and becoming a routine generating single higher order Arabidopsis thaliana mutants. Low entry, reliable assembly CRISPR/Cas9 vectors mutagenesis necessary to enable maximum researchers break through the genetic redundancy within plant multi-gene families allow plethora studies that have been previously...
Plants continuously face various environmental stressors throughout their lifetime. To be able to grow and adapt in different environments, they developed specialized tissues that allowed them maintain a protected yet interconnected body. These undergo specific primary secondary cell wall modifications are essential ensure normal plant growth, adaptation successful land colonization. The composition of walls can vary among species, organs tissues. ability remodel is fundamental for plants...
Precise coordination between cells and tissues is essential for differential growth in plants. During lateral root formation Arabidopsis thaliana , the endodermis actively remodeled to allow outgrowth of new organ. Here, we show that microtubule arrays facing founder display a higher order compared on opposite side same cell, this asymmetry required endodermal remodeling initiation. We identify MICROTUBULE ASSOCIATED PROTEIN 70-5 (MAP70-5) necessary establishment spatially defined...
The plant cell wall is a plastic structure of variable composition that constitutes the first line defence against environmental challenges. Lodging and drought are two stressful conditions severely impact maize yield. In previous work, we characterised walls inbreds, EA2024 (susceptible) B73 (resistant) to stalk lodging. Here, show induces distinct phenotypical, physiological, wall, transcriptional changes in with exhibiting lower tolerance this stress than EA2024. control conditions,...
Abstract Multicellular organisms control environmental interactions through specialized barriers in specific cell types. A conserved barrier plant roots is the endodermal Casparian strip (CS), a ring-like structure made of polymerized lignin that seals apoplastic space. Most angiosperms have another root type, exodermis, reported to form barrier. Our understanding exodermal developmental and molecular regulation function limited as this type absent from Arabidopsis thaliana . We demonstrate...
ABSTRACT Multicellular organisms control interactions with their environment through the development of specialized barriers in specific cell types. A conserved barrier plant roots is endodermal Casparian strip (CS). The CS made polymerized lignin and forms a ring-like structure that seals apoplastic space between cells. Most angiosperms also have another root type, exodermis, reported to form barrier. Our understanding exodermal developmental molecular regulation, as well function, limited...
Abstract The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed protophloem developmental trajectory of 19 from birth terminal differentiation at single resolution Arabidopsis root. We found cellular specification is mediated near stem niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, PEAR dependent program repressed a broad gradient...
SUMMARY Plant roots integrate environmental signals and developmental programs using exquisite spatiotemporal control. This is apparent in the deposition of suberin, an apoplastic diffusion barrier, which regulates entry exit water, solutes gases, environmentally plastic. Suberin considered a hallmark endodermal differentiation, but we find that it absent tomato endodermis during normal development. Instead, suberin present exodermis, cell type model organism Arabidopsis thaliana . Here,...
Stress granules (SGs) are ribonucleoprotein functional condensates that form under stress conditions in all eukaryotic cells. Although their stress-survival function is far from clear, SGs have been implicated the regulation of many vital cellular pathways. Consequently, SG dysfunction thought to be a mechanistic point origin for neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Additionally, play role pathogenic pathways as diverse viral infection and chemotherapy...