A5047573198- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Surface Properties and Treatments
- Plant Parasitism and Resistance
- RNA Research and Splicing
- Nematode management and characterization studies
- Tree Root and Stability Studies
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Light effects on plants
- Algal biology and biofuel production
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Magnetic and Electromagnetic Effects
- Glycosylation and Glycoproteins Research
- Polymer-Based Agricultural Enhancements
- Postharvest Quality and Shelf Life Management
Swedish University of Agricultural Sciences
2020-2025
Umeå Plant Science Centre
2020-2025
University of Lausanne
2017-2022
Institute of Science and Technology Austria
2013-2021
Ghent University
2010-2014
VIB-UGent Center for Plant Systems Biology
2011-2014
Vlaams Instituut voor Biotechnologie
2010-2012
Higher plant function is contingent upon the complex three-dimensional (3D) architecture of tissues, yet severe light scattering renders deep, 3D tissue imaging very problematic. Although efforts to 'clear' tissues have been ongoing for over a century, many innovations made in recent years. Among them, protocol called ClearSee efficiently clears and diminishes chlorophyll autofluorescence while maintaining fluorescent proteins - thereby allowing analysis gene expression protein localisation...
The apical hook of dark-grown Arabidopsis seedlings is a simple structure that develops soon after germination to protect the meristem tissues during emergence through soil and opens upon exposure light. Differential growth at proceeds in three sequential steps are regulated by multiple hormones, principally auxin ethylene. We show progress these developmental phases depends on dynamic, asymmetric distribution auxin, which efflux carriers PIN family. Several proteins exhibited specific,...
Reactivation of cell division is crucial for the regeneration damaged tissues, which a fundamental process across all multicellular organisms. However, mechanisms underlying activation in plants during remain poorly understood. Here, we show that single-cell endodermal ablation generates transient change local mechanical pressure on neighboring pericycle cells to activate patterned tissue Arabidopsis roots. Moreover, provide strong evidence this relies phytohormone ethylene. Thus, our...
The architecture of a plant's root system, established postembryonically, results from both coordinated growth and lateral branching. plant hormones auxin cytokinin are central endogenous signaling molecules that regulate organogenesis positively negatively, respectively. Tight control mutual balance their antagonistic activities particularly important during the early phases to ensure continuous initiation (LRI) proper development primordia (LRP). Here, we show organogenesis, including...
Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation auxin distribution is a developmentally crucial hormonal interaction, its molecular basis largely unknown. Here we show direct regulatory link between signalling the transport machinery uncovering mechanistic framework for cytokinin-auxin cross-talk. We that CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream perception, transcriptionally control genes encoding...
Patterning in plants relies on oriented cell divisions and acquisition of specific identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism restorative patterning that repairs wounding. Laser-assisted elimination different cells Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis the regeneration...
Article18 March 2020Open Access Transparent process SCHENGEN receptor module drives localized ROS production and lignification in plant roots Satoshi Fujita Corresponding Author [email protected] orcid.org/0000-0002-3514-3349 Department of Plant Molecular Biology, Biophore, University Lausanne, Switzerland Search for more papers by this author Damien De Bellis orcid.org/0000-0001-9480-7208 Electron Microscopy Facility, Kai H Edel Institut für Biologie und Biotechnologie der Pflanzen,...
To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with preserved proliferation capacity. The root pericycle represents unique tissue conditional meristematic activity, and its tight control determines initiation lateral organs. Here we show that the activity is constrained by interaction adjacent endodermis. Release these restraints elimination endodermal single-cell ablation triggers re-enter cell cycle. We found endodermis removal substitutes...
Plant-parasitic nematodes (PPNs) cause tremendous yield losses worldwide in almost all economically important crops. The agriculturally most PPNs belong to a small group of root-infecting sedentary endoparasites that includes cyst and root-knot nematodes. Both induce specialized long-term feeding structures root vasculature from which they obtain their nutrients. A cell layer roots called the endodermis, has walls reinforced with suberin deposits lignin-based Casparian strip (CS), protects...
Abstract Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization receptors proposed endoplasmic reticulum (ER) membrane as principal cytokinin perception site, while study transport pointed to plasma (PM)-mediated signalling. Here, detailed monitoring subcellular localizations fluorescently labelled natural probe and receptor ARABIDOPSIS...
Suberin is a fundamental plant biopolymer, found in protective tissues, such as seed coats, exodermis and endodermis of roots. deposited most suberizing cells the form lamellae just outside plasma membrane, below primary cell wall. How monomeric suberin precursors, thought to be synthesized at endoplasmic reticulum, are transported cell, for polymerization into has remained obscure. Using electron-microscopy, we observed large numbers extracellular vesiculo-tubular structures (EVs)...
Abstract Multiple plant developmental processes, such as lateral root development, depend on auxin distribution patterns that are in part generated by the PIN-formed family of auxin-efflux transporters. Here we propose AUXIN RESPONSE FACTOR7 (ARF7) and ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly form a coherent feed-forward motif mediates auxin-responsive PIN3 planta to steer early steps formation. This regulatory mechanism might endow circuitry with temporal ‘memory’...
Abstract Plant–parasitic nematodes, specifically cyst nematodes (CNs) and root‐knot (RKNs), pose significant threats to global agriculture, leading substantial crop losses. Both CNs RKNs induce permanent feeding sites in the root of their host plants, which then serve as only source nutrients throughout lifecycle. Plants deploy reactive oxygen species (ROS) a primary defense mechanism against nematode invasion. Notably, both have evolved sophisticated strategies manipulate host's redox...
Phytohormones are important plant growth regulators that control many developmental processes, such as cell division, differentiation, organogenesis and morphogenesis. They regulate a multitude of apparently unrelated physiological often with overlapping roles, they mutually modulate their effects. These features imply synergistic antagonistic interactions between the various hormones. Auxin cytokinin central hormones involved in regulation development, including processes determining root...
Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed plants. Here, show that correct establishment Casparian Strip (CS) network relies on local neighbor communication. We positioning membrane domains (CSDs) is tightly coordinated between neighbors wild-type restriction domain formation involves...