A5061573426- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Insect-Plant Interactions and Control
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Insect behavior and control techniques
- Seed Germination and Physiology
- Calcium signaling and nucleotide metabolism
- Plant Parasitism and Resistance
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Plant Growth Enhancement Techniques
- Plant Virus Research Studies
- Polymer-Based Agricultural Enhancements
- Natural product bioactivities and synthesis
- Composting and Vermicomposting Techniques
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant responses to elevated CO2
- Mycorrhizal Fungi and Plant Interactions
- Light effects on plants
Universitat Jaume I
2019-2025
European Union
2021
Public Risk Management Association
2021
Instituto de Biología Molecular y Celular de Plantas
2004-2020
Universitat Politècnica de València
2006-2020
Centro de Investigaciones Biológicas Margarita Salas
2016-2020
Consejo Superior de Investigaciones Científicas
2002-2017
Planta
2016
VIB-UGent Center for Plant Systems Biology
2009-2013
Ghent University
2007-2013
Abscisic acid (ABA) is a key hormone for plant growth, development, and stress adaptation. Perception of ABA through four types receptors has been reported. We show here that impairment perception the PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF RECEPTORS (RCAR) branch reduces vegetative growth seed production leads to severe open stomata ABA-insensitive phenotype, even though other branches remain functional. An Arabidopsis thaliana sextuple mutant impaired in six...
Abstract Mutants able to germinate and perform early growth in medium containing a high NaCl concentration were identified during the course of two independent screenings named salt resistant (sre) salobreño (sañ). The sre sañ mutants also high-osmoticum medium, indicating that they are osmotolerant germination assay. Complementation analyses revealed sre1-1, sre1-2, sañ3-1, sañ3-2 alleles abscisic acid (ABA) biosynthesis ABA2 gene. A map-based cloning strategy allowed identification...
Summary HAB1 was originally cloned on the basis of sequence homology to ABI1 and ABI2 , indeed, a multiple alignment 32 Arabidopsis protein phosphatases type‐2C (PP2Cs) reveals cluster composed by four closely related proteins, ABI1, ABI2, At1g17550 (here named HAB2). Characterisation transgenic plants harbouring transcriptional fusion Pro : green fluorescent ( GFP ) indicates that is broadly expressed within plant, including key target sites abscisic acid (ABA) action as guard cells or...
Plant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but molecular mechanisms underlying this are poorly understood. Stress-derived energy deprivation activates Snf1-related protein kinases1 (SnRK1s), triggering vast transcriptional and metabolic reprogramming that restores homeostasis promotes tolerance to adverse conditions. Here, we show two clade A type 2C phosphatases (PP2Cs), established repressors abscisic acid...
Abscisic acid (ABA) signaling plays a critical role in regulating root growth and system architecture. ABA-mediated promotion tropic response under water stress are key responses for plant survival limiting conditions. In this work, we have explored the of Arabidopsis (Arabidopsis thaliana) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS signaling. As result, discovered that PYL8 nonredundant regulation sensitivity. Unexpectedly, given multigenic nature...
The phytohormones jasmonates (JAs) constitute an important class of elicitors for many plant secondary metabolic pathways. However, JAs do not act independently but operate in complex networks with crosstalk to several other phytohormonal signaling Here, was detected between the JA and abscisic acid (ABA) pathways regulation tobacco ( Nicotiana tabacum ) alkaloid biosynthesis. A gene from PYR/PYL/RCAR family, NtPYL4 , expression which is regulated by JAs, found encode a functional ABA...
Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly generation new varieties with improved water use efficiency drought tolerance. Different studies have shown potential applications Arabidopsis PYR/PYL/RCAR ABA receptors enhance plant resistance. Consequently functional characterization orthologous genes crops holds promise for agriculture. The full set tomato (Solanum...
Much of the literature on phytohormone abscisic acid (ABA) describes it as a mediator in triggering plant responses to environmental stimuli, well growth inhibitor. ABA-deficient mutants, however, display stunted phenotype even under well-watered conditions and high relative humidity, which suggests that promotion may also be one roles endogenous ABA. Zeaxanthin epoxidase, product ABA1 gene Arabidopsis thaliana, catalyses epoxidation zeaxanthin antheraxanthin violaxanthin, generating...
Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence a previously uncharacterized member this PP2C family Arabidopsis (Arabidopsis thaliana), At5g59220, is regulator osmotic stress and function was only apparent when double loss-of-function mutants with...
Rapid stomatal closure induced by changes in the environment, such as elevation of CO2, reduction air humidity, darkness, and pulses pollutant ozone (O3), involves SLOW ANION CHANNEL1 (SLAC1). SLAC1 is activated OPEN STOMATA1 (OST1) Ca(2+)-dependent protein kinases. OST1 activation controlled through abscisic acid (ABA)-induced inhibition type 2 phosphatases (PP2C) PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR) receptor proteins. To address role signaling PYR/RCARs...
Membrane-delimited events play a crucial role for ABA signaling and PYR/PYL/RCAR receptors, clade A PP2Cs SnRK2/CPK kinases modulate the activity of different plasma membrane components involved in action. Therefore, turnover PYR/PYL/RCARs proximity might be step that affects receptor function downstream signaling. In this study we describe single-subunit RING-type E3 ubiquitin ligase RSL1 interacts with PYL4 PYR1 receptors at membrane. Overexpression reduces sensitivity rsl1 RNAi lines...
Abstract Because abscisic acid (ABA) is recognized as the critical hormonal regulator of plant stress physiology, elucidating its signaling pathway has raised promise for application in agriculture, instance through genetic engineering ABA receptors. PYRABACTIN RESISTANCE1/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF RECEPTORS receptors interact with high affinity and inhibit clade A phosphatases type-2C (PP2Cs) an ABA-dependent manner. We generated allele library composed 10,000 mutant clones...
Nitric oxide may limit ABA signaling in plants by rapid inactivation of the receptors through tyrosine nitration.
Recently, we described the ubiquitylation of PYL4 and PYR1 by RING E3 ubiquitin ligase RSL1 at plasma membrane Arabidopsis thaliana This suggested that ubiquitylated abscisic acid (ABA) receptors might be targeted to vacuolar degradation pathway because such is usually an internalization signal for endocytic route. Here, show FYVE1 (previously termed FREE1), a recently component endosomal sorting complex required transport (ESCRT) machinery, interacted with RSL1-receptor complexes recruited...
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits unique ability invade adapt ecological niches throughout tropical subtropical regions the world, though medfly infestations have been prevented controlled by sterile technique (SIT) as part integrated management programs (IPMs). genetic analysis manipulation has subject intensive study in an effort improve SIT...
Membrane-delimited abscisic acid (ABA) signal transduction plays a critical role in early ABA signaling, but the molecular mechanisms linking core signaling components to plasma membrane are unclear. We show that transient calcium-dependent interactions of PYR/PYL receptors with membranes mediated through 10-member family C2-domain ABA-related (CAR) proteins Arabidopsis thaliana. Specifically, we found PYL4 interacted an ABA-independent manner CAR1 both and nucleus plant cells. belongs...
Abstract The plant hormone abscisic acid (ABA) plays a crucial role in the control of stress response and regulation growth development. ABA binding to PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF RECEPTORS intracellular receptors leads inhibition key negative regulators signaling, i.e. clade A protein phosphatases type 2C (PP2Cs) such as ABA-INSENSITIVE1 HYPERSENSITIVE TO ABA1 (HAB1), causing activation signaling pathway. To gain further understanding on mechanism...
The stress hormone abscisic acid (ABA) induces expression of defence genes in many organs, modulates ion homeostasis and metabolism guard cells, inhibits germination seedling growth. Concerning the latter effect, several mutants Arabidopsis thaliana with improved capability for H+ efflux (wat1-1D, overexpression AKT1 ost2-1D) are less sensitive to inhibition by ABA than wild type. This suggested that could inhibit (H+-ATPase) induce cytosolic acidification as a mechanism growth inhibition....
Significance Drought and salinity are the major threats to crop productivity at a worldwide scale. A fundamental portion of plant response these environmental stresses occurs cell membrane, where molecular machinery preserve turgor appropriate balance intracellular ions is found. The C2-domain ABA-related (CAR) family proteins contributes processes by delivering regulatory controlling this from other compartments membrane. Our analysis provides an explanation on how CAR specifically reach...