A5006303034- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Ecology and Vegetation Dynamics Studies
- Protist diversity and phylogeny
- Light effects on plants
- Plant Taxonomy and Phylogenetics
- Plant Water Relations and Carbon Dynamics
- Botany and Plant Ecology Studies
- Fern and Epiphyte Biology
- Cardiovascular Effects of Exercise
- Viral Infections and Immunology Research
- Microplastics and Plastic Pollution
- Recycling and Waste Management Techniques
- Lichen and fungal ecology
- Biocrusts and Microbial Ecology
- Seed Germination and Physiology
- Marine Biology and Environmental Chemistry
- Plant and animal studies
- Leaf Properties and Growth Measurement
University of Wisconsin–Oshkosh
2021-2024
Maine-Dartmouth Family Medicine Residency
2022
Southern Illinois University Carbondale
2002-2017
University of British Columbia
2011-2015
The Ohio State University
2005-2015
Santa Clara University
2013
Indiana University Bloomington
2008-2012
Indiana University
2008
The two guard cells of a stoma are produced by single symmetric division just before terminal differentiation. Recessive mutations in the FOUR LIPS (FLP) gene abnormally induce at least four contact with one another. These pattern defects result from persistence precursor cell identity that leads to extra divisions end lineage. FLP is likely be required for correct timing transition cycling encodes two-repeat (R2R3) MYB protein whose expression accumulates division. A paralogous gene, MYB88,...
Abstract Stomata, which are epidermal pores surrounded by two guard cells, develop from a specialized stem cell lineage and function in shoot gas exchange. The Arabidopsis thaliana FOUR LIPS (FLP) MYB88 genes encode closely related atypical two-MYB-repeat proteins, when mutated result excess divisions abnormal groups of stomata contact. Consistent with role transcription, we show here that FLP nuclear proteins DNA binding preferences distinct other known MYBs. To identify possible FLP/MYB88...
Abstract The Arabidopsis thaliana MAP65-1 and MAP65-2 genes are members of the larger eukaryotic MAP65/ASE1/PRC gene family microtubule-associated proteins. We created fluorescent protein fusions driven by native promoters that colocalized to a subset interphase microtubule bundles in all epidermal hypocotyl cells. labeling was highly dynamic within bundles, showing episodes linear extension retraction coincident with growth shortening. Dynamic colocalization MAP65-1/2 polymerizing...
Abstract Multiple plant developmental processes, such as lateral root development, depend on auxin distribution patterns that are in part generated by the PIN-formed family of auxin-efflux transporters. Here we propose AUXIN RESPONSE FACTOR7 (ARF7) and ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly form a coherent feed-forward motif mediates auxin-responsive PIN3 planta to steer early steps formation. This regulatory mechanism might endow circuitry with temporal ‘memory’...
Abstract The acentriolar cortical microtubule arrays in dark-grown hypocotyl cells organize into a transverse coaligned pattern that is critical for axial plant growth. In light-grown Arabidopsis thaliana seedlings, the array on outer (periclinal) cell face creates variety of patterns with significant bias (>3:1) microtubules polymerizing edge-ward and side (anticlinal) faces cell. To study mechanisms required creating coalignment, we developed dual-hormone protocol synchronously...
As one of the earliest plant groups to evolve stomata, hornworts are key understanding origin and function stomata. Hornwort stomata large scattered on sporangia that grow from their bases release spores at tips. We present data development immunocytochemistry identify a role for hornwort is correlated with sporangial spore maturation. measured guard cells across genera assess developmental changes in size analyze any correlation genome size. Stomata form base sporophyte green region, where...
Summary Arabidopsis guard cell ( GC ) fate is conferred via a transient pulse of expression FAMA that encodes bHLH transcription factor. Stomata often function for years, suggesting the window stabilizes long‐term identity or additional factors operate. Transgenic lines harboring copy transgene were found to induce resetting mature s lineage‐specific stem cells causing new stomata arise within shells old, Stoma‐in‐Stoma SIS phenotype. These disrupt normal trimethylation on lysine 27 histone3...
Microtubule arrays in living cells were analysed during Arabidopsis stomatal development order to more closely define stages the pathway and contexts where intercellular signalling might operate. stomata are patterned iteratively via orientation of an asymmetric division a cell located next existing stoma. It was found that preprophase bands microtubules (PPBs) correctly placed away from two types precursor cells. This suggests all three participate orients site. These other divisions...
Summary We investigated the role of Arabidopsis microtubule associated proteins 65‐1 and 65‐2 (MAP65‐1 MAP65‐2) in control axial root growth. Transgenic plants expressing fluorescent fusion from native promoters indicated exactly overlapping accumulation MAP65‐1 MAP65‐2 tip elongation zone. Nearly identical protein patterns were observed when expressed behind a constitutive CaMV 35S promoter, suggesting level post‐transcriptional that restricts these to rapidly growing portions root....
Myxospermy, the release of seed mucilage upon hydration, plays multiple roles in biology. Here, we explore whether occurs a suite temperate grassland species to test if prevalence producing is associated with habitat type or characteristics. Seventy plant found wet dry North American grasslands were tested for presence through microscopic examination seeds imbibed histochemical stain mucilage. Mucilage production was compared among different moisture requirements and mass. In this study, 43...
Abstract We tested the general hypothesis that bundling stabilizes dynamic properties of constituent microtubules (MTs) in vivo. quantified assembly dynamics bundled and unbundled MTs interphase cortical array Arabidopsis hypocotyl cells using high range spinning disk confocal microscopy. find no evidence are stabilized against depolymerization through changes to their properties. Our observations MT plus minus ends indicate both polymers undergo persistent treadmilling this system. conclude...
Dynein motor proteins, often considered to be missing in land plants, are found plants that reproduce with flagellated sperm.
Journal Article Structure and Function of Hornwort Stomata Get access Jessica R Lucas, Lucas Department Plant Biology, Southern Illinois University, Carbondale, IL 62901 Search for other works by this author on: Oxford Academic Google Scholar Karen S Renzaglia Microscopy Microanalysis, Volume 8, Issue S02, 1 August 2002, Pages 1090–1091, https://doi.org/10.1017/S143192760210701X Published: 01 2002
Stomata display a mirror-like symmetry that is adaptive for shoot/atmosphere gas exchange. This includes the facing guard cells around lens-shaped and bilaterally symmetric pore, as well radially arranged microtubule arrays primarily originate at pore then grow outwards. Mutations in MUSTACHES (MUS), which encodes leucine-rich repeat receptor-like kinase, disrupt this symmetry, resulting defects ranging from skewed pores abnormally focused depolarized radial arrays, to paired face away each...
Preprophase bands are belts of cortical microtubules that appear at the end interphase and predict where cell plates will fuse with parental walls during division. Phragmoplasts microtubule-rich arrays orchestrate growth guidance cytokinesis. Descriptions development these often assume non-polar formation, preprophase developing more or less simultaneously around circumference. described as initiating center then expanding evenly outwards until fusion parent walls. We analyzed...
Abstract Microtubule arrays drastically reorganize during the cell cycle to facilitate specific events. Many cells contain a centrosome that dictates assembly and organization of microtubule arrays. However, plant many others do not centrosomes or discrete organizing centers. In plants, microtubules nucleate polymerize from gamma‐tubulin‐containing complexes in interphase cortex. During division, near nuclei form mitotic spindle plant‐specific phragmoplast required for cytokinesis....
Self incompatibility (SI) in rare plants presents a unique challenge-SI protects from inbreeding depression, but requires sufficient number of mates and xenogamous pollination. Does SI persist an endangered polyploid? Is pollinator visitation to ensure reproductive success? there evidence inbreeding/outbreeding depression? We characterized the mating system, primary pollinators, pollen limitation, depression Erysimum teretifolium guide conservation efforts.We compared seed production...
Stomatal pores are adjustable microscopic holes on the surface of photosynthetic tissues that help regulate multiple aspects plant physiology. facilitate gas exchange necessary for photosynthesis, water transport, and temperature regulation. Pore size is influenced by many intertwined environmental, molecular, cellular, physiological cues. Accurate precise measurements pore important understanding mechanisms adjust Here we investigate whether conventional width appropriate economically crop...
Microtubules are essential components of eukaryotic cells. Myriad proteins associate with microtubules to facilitate the organization and operation microtubule arrays. Various M icrotubule A ssociated P roteins (MAPs) assist assembly function mitotic spindles interphase Nine MAP65 genes exist in genome acentrosomal model plant, Arabidopsis thaliana, majority these is unclear. To address this knowledge gap, we demonstrate localization A. thaliana MAP65-6 MAP65-7 fusion expressed from native...