A5101656373- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Cellular transport and secretion
- Plant Stress Responses and Tolerance
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Metabolism and Genetic Disorders
- Eicosanoids and Hypertension Pharmacology
- Plant-Microbe Interactions and Immunity
- Pneumocystis jirovecii pneumonia detection and treatment
- dental development and anomalies
- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Oral and Craniofacial Lesions
- Trace Elements in Health
- Endoplasmic Reticulum Stress and Disease
- Bone and Dental Protein Studies
- Dermatological and Skeletal Disorders
- DNA and Nucleic Acid Chemistry
- Oral and Maxillofacial Pathology
Inha University
2022
University of British Columbia
2009-2020
Ghent University
2015
Vlaams Instituut voor Biotechnologie
2015
Gyeongsang National University
2009
Eukaryotic endoplasmic reticulum (ER)-plasma membrane (PM) contact sites are evolutionarily conserved microdomains that have important roles in specialized metabolic functions such as ER-PM communication, lipid homeostasis, and Ca2+ influx. Despite recent advances knowledge about site components yeast (Saccharomyces cerevisiae) mammals, relatively little is known the functional significance of these structures plants. In this report, we characterize Arabidopsis (Arabidopsis thaliana)...
Abstract Cytokinesis is the division of cytoplasm and its separation into two daughter cells. Cell plate growth cytokinesis appear to require callose, but direct functional evidence still lacking. To determine role callose synthesis during cytokinesis, we identified characterized mutants in many members GLUCAN SYNTHASE-LIKE (GSL; or CALLOSE SYNTHASE) gene family Arabidopsis (Arabidopsis thaliana). Most gsl (gsl1–gsl7, gsl9, gsl11, gsl12) exhibited roughly normal seedling development....
In multicellular organisms, morphogenesis relies on a strict coordination in time and space of cell proliferation differentiation. contrast to animals, plant development displays continuous organ formation adaptive growth responses during their lifespan relying tight proliferation. How developmental signals interact with the cell-cycle machinery is largely unknown. Here, we characterize A2-type cyclins, small gene family mitotic show how they contribute fine-tuning local development....
Significance Interorganelle connectivity and nonvesicular information transfer are hallmarks of biological systems. These processes facilitate communication between organelles, allowing them to adapt changing cellular environments. In plants, the endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (EPCSs) physically connect cortical ER PM, act as general platforms for Ca 2+ homeostasis regulation adaptation environmental stresses. Our identification ionic stress PM...
Abstract Multiple plant developmental processes, such as lateral root development, depend on auxin distribution patterns that are in part generated by the PIN-formed family of auxin-efflux transporters. Here we propose AUXIN RESPONSE FACTOR7 (ARF7) and ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly form a coherent feed-forward motif mediates auxin-responsive PIN3 planta to steer early steps formation. This regulatory mechanism might endow circuitry with temporal ‘memory’...
Nucleocytoplasmic trafficking is emerging as an important aspect of plant immunity. The three related pathways affecting immunity include Nuclear Localization Signal (NLS)-mediated nuclear protein import, Export (NES)-dependent export, and mRNA export relying on MOS3, a nucleoporin belonging to the Nup107-160 complex. Here we report characterization, identification, detailed analysis Arabidopsis modifier snc1, 11 (mos11). Mutations in MOS11 can partially suppress dwarfism enhanced disease...
Summary Arabidopsis guard cell ( GC ) fate is conferred via a transient pulse of expression FAMA that encodes bHLH transcription factor. Stomata often function for years, suggesting the window stabilizes long‐term identity or additional factors operate. Transgenic lines harboring copy transgene were found to induce resetting mature s lineage‐specific stem cells causing new stomata arise within shells old, Stoma‐in‐Stoma SIS phenotype. These disrupt normal trimethylation on lysine 27 histone3...
Summary Functional redundancy arises between gene paralogs as well non‐homologous genes that play a common role at shared node. The bHLH transcription factor FAMA, along with the paralogous MYB genes, FOUR LIPS ( FLP ) and MYB88 all ensure Arabidopsis stomata contain just two guard cells (GCs) by enforcing single symmetric precursor cell division before stomatal maturity. Consistent this function, FAMA exhibit same expression pattern in which both translational GFP fusions emit fluorescence...
In plant cells, environmental stressors promote changes in connectivity between the cortical endoplasmic reticulum (ER) and plasma membrane (PM). Although this process is tightly regulated space time, molecular signals structural components mediating these interorganelle communication are only starting to be characterized. report, we confirm presence of a putative tethering complex containing synaptotagmins 1 5 (SYT1 SYT5) Ca2+- lipid-binding protein (CLB1/SYT7). This enriched at ER-PM...
Gamete formation is an important step in the life cycle of sexually reproducing organisms. In flowering plants, haploid spores are formed after meiotic division spore mother cells. These develop into male and female gametophytes containing gametes undergoing mitotic divisions. female, megaspore cell undergoes meiosis forming four megaspores, which one functional three degenerate. The then cycles thus generating embryo sac with eight nuclei. cellularization to form mature seven-celled...
Consistent with their valve-like function in shoot–atmosphere gas exchange, guard cells are smaller than other epidermal and usually harbour 2C DNA levels diploid plants. The paralogous Arabidopsis R2R3 MYB transcription factors, FOUR LIPS MYB88, ensure that stomata contain just two by restricting mitosis. loss of both FLP MYB88 flp myb88 double mutants induces repeated mitotic divisions lead to the formation clusters direct contact. By contrast, CYCLIN DEPENDENT KINASE B1 is required for...
PREMISE OF THE STUDY: The FOUR LIPS (FLP) and MYB88 transcription factors, which are closely related in structure function, control the development of stomata, as well entry into megasporogenesis Arabidopsis thaliana . However, other locations where these factors expressed poorly described. Documenting additional genes might define new functions for genes. METHODS: Expression patterns were examined throughout vegetative reproductive development. expression from two transcriptional‐reporter...
Although diverse cell penetrating motifs not only from naturally occurring proteins but also synthetic peptides have been discovered and developed, the selectivity of cargo delivery connected to these into desired target cells is generally low. Here, we demonstrate selective cytotoxicity tuning an anticancer KLA peptide with a motif activatable by matrix metalloproteinase-2 (MMP2). The anionic masking sequence introduced at end through MMP2-cleavable linker selectively cleaved MMP2 cationic...
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Abstract Lipocalin 2 (Lcn2) has long been regarded to induce cellular proliferation because expression of Lcn2 associated in a variety proliferative cells. Consistent with this, it demonstrated the present study that was significantly elevated human HCC (hepatocellular carcinoma) tissues compared non-tumor liver tissues. However, roles hepatocarcinogenesis are far from clear. To investigate effects on hepatocarcinogenesis, Chang and SK-Hep1, cell lines, were genetically manipulated express...
physiology or medicine 50 years ago for the discovery of DNA double helix.That finding is well known to have been important development molecular biology.However, it's not widely appreciated that Crick and an-
ABSTRACT In plant cells, environmental stressors induce changes in the cytosolic concentration of calcium ([Ca 2+ ] cyt ) that are transduced by Ca -sensing proteins. To confer specificity to stress signaling response, [Ca sensing must be tightly regulated space and time; molecular mechanisms restrict localization dynamics sensors plants, however, largely unknown. this report, we identify a putative -sensitive complex containing synaptotagmins 1 5 (SYT1 SYT5) -dependent lipid binding protein...