A5084968904- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- Polysaccharides Composition and Applications
- Postharvest Quality and Shelf Life Management
- Plant Disease Management Techniques
- Seed Germination and Physiology
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Advanced Cellulose Research Studies
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Food composition and properties
- Crop Yield and Soil Fertility
- Growth and nutrition in plants
- Plant Reproductive Biology
- GABA and Rice Research
- Legume Nitrogen Fixing Symbiosis
- Agricultural Science and Fertilization
- Plant Molecular Biology Research
- Lignin and Wood Chemistry
- Microbial Metabolites in Food Biotechnology
- Seaweed-derived Bioactive Compounds
- Educational Innovations and Technology
Universidad de León
2016-2025
Universidad de León
2011-2024
The plant cell wall is a plastic structure of variable composition that constitutes the first line defence against environmental challenges. Lodging and drought are two stressful conditions severely impact maize yield. In previous work, we characterised walls inbreds, EA2024 (susceptible) B73 (resistant) to stalk lodging. Here, show induces distinct phenotypical, physiological, wall, transcriptional changes in with exhibiting lower tolerance this stress than EA2024. control conditions,...
Abstract Maize ( Zea mays L.) suspension‐cultured cells with up to 70% less cellulose were obtained by stepwise habituation dichlobenil (DCB), a biosynthesis inhibitor. Cellulose deficiency was accompanied marked changes in cell wall matrix polysaccharides and phenolics as revealed Fourier transform infrared (FTIR) spectroscopy. Cell compositional analysis indicated that the cellulose‐deficient walls showed an enhancement of highly branched cross‐linked arabinoxylans, well increased content...
The P. pinaster species, known as 'Pino nigral or rodeno', is used in the treatment of colds, asthma, flu, and tuberculosis.This study determined anti-inflammatory, analgesic, antibacterial activities resin, identifying compounds with higher biological activity.A bio-guided isolation was carried out by selecting most active extracts anti-inflammatory analgesic effects HBEC3-KT, MRC-5, THP-1 cell lines. activity against S. aureus, pneumoniae, K. pneumoniae aeruginosa strains.The following...
Grafting is a horticultural technique that involves healing process requires grafted plants to develop physiological responses overcome oxidative stress. In this study, damage, total antioxidant capacity and enzymatic activities were analysed in functional non-functional tomato autografts for eight days after grafting, considering scion rootstock tissues separately. The results showed measured as lipid peroxidation, was controlled, especially grafts. Scion significant increases of key...
Bean (Phaseolus vulgaris L.) cell suspensions were adapted for growth in 12 &mgr;M dichlobenil (2,6-dichlorobenzonitrile or DCB) by a stepwise increase the concentration of inhibitor each subculture. Non-tolerant (I50 = 0.3 &mgr;M) gave rise to single cells small clusters while tolerant 30 grown DCB formed large clusters. The these surrounded thick and irregular wall with lamellate structure lacking differentiated middle lamella. Analysis habituated walls Fourier transform infrared...
A large part of the production tomato plants is grafted. Although it has recently been described that cell walls play an important role in graft healing, spatiotemporal dynamics wall changes this critical process remains largely unknown. The aim work was to immunolocalize major matrix components autograft union tissues throughout course from 1 20 days after grafting (DAG). Homogalacturonan de novo synthetized and deposited cut edges, displaying low methyl-esterified homogalacturonan a...
Grafting is a technique applied to considerable number of crops, with tomato standing out. However, this limited by the obtaining unfunctional grafts, which decrease success rate and therefore benefits achieved. The aim work was analyze failure in intraspecific grafting plants, focusing on tissue development, cell wall defense reactions, distribution starch soluble sugars at graft junction. autografts higher than that homografts heterografts. Unfunctional heterografts showed similar...
Grafting involves a sequence of modifications that may vary according to genotypes, grafting techniques and growing conditions. This process is often monitored using destructive methods, precluding the possibility monitoring entire in same grafted plant. The aim this study was test effectiveness two non-invasive methods-thermographic inference transpiration determination chlorophyll quantum yields-for graft dynamics tomato (Solanum lycopersicum L.) autografts compare results with other...
The effects of the cellulose inhibitor dichlobenil on cell wall composition and structure during habituation/dehabituation process suspension‐cultured bean cells were assessed. A range techniques used including fractionation, sugar analysis, immunofluorescence fluorochrome labelling resin‐embedded sections, immunodot assays (IDAs) fractions. walls from suspensions with initial levels habituation to had decreased cellulose, but this effect lessened increasing numbers subcultures. All analysed...
Many phytopathogenic fungi produce necrosis and ethylene inducing peptide 1 (Nep1-like proteins or NLP) that trigger leaf the activation of defense mechanisms. These have been widely studied in plant pathogens as Moniliophthora perniciosa Botrytis cinerea between others, but little is known about their biological roles grapevine trunk pathogens. Advances sequencing genomes several involved diseases revealed these are present copies genomes. The aim this project was to analyze presence genes...
The variety of bioassays developed to evaluate different inhibition responses for cellulose biosynthesis inhibitors makes it difficult compare the results obtained. This work aims (i) test a single inhibitory assay comparing active concentrations set putative and (ii) characterize their effect on cell wall polysaccharides following short-term exposure. For first aim, dose-response curves dry-weight increase 30 days exposure bean callus-cultured cells these were compound concentration capable...