A5008839289- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects and Toxicity Studies
- HIV/AIDS drug development and treatment
- Effects and risks of endocrine disrupting chemicals
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Molecular Sensors and Ion Detection
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Animal Virus Infections Studies
- Pharmacogenetics and Drug Metabolism
- Metabolism and Genetic Disorders
- Viral Infections and Immunology Research
- Cytomegalovirus and herpesvirus research
- Chemical Synthesis and Analysis
Austrian Academy of Sciences
2019-2025
CeMM Research Center for Molecular Medicine
2019-2023
Abstract Solute carrier (SLC) transporters govern most of the chemical exchange across cellular membranes and are integral to metabolic regulation, which in turn is linked function identity. Despite their key role, individual functions SLC superfamily members were not evaluated systematically. We determined transcriptional profiles upon overexpression knock-out or wild-type isogenic cell backgrounds for 378 SLCs 441 SLCs, respectively. Targeted metabolomics provided a fingerprint 189...
Abstract Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in variety subcellular membranes. Loss-of-function an unusually high proportion SLC is genetically associated with plethora human diseases, making SLCs rapidly emerging but challenging drug target class. Knowledge their environment may elucidate the molecular basis for functional...
Abstract About a thousand genes in the human genome encode for membrane transporters. Among these, several solute carrier proteins (SLCs), representing largest group of transporters, are still orphan and lack functional characterization. We reasoned that assessing genetic interactions among SLCs may be an efficient way to obtain information allowing their deorphanization. Here we describe network strong indicating contribution mitochondrial respiration redox metabolism SLC25A51/MCART1,...
Solute carrier (SLC) transporters govern most of the chemical exchange across cellular membranes and are integral to metabolic regulation, which in turn is linked function identity. Despite their key role, individual functions members SLC superfamily were not evaluated systematically. We determined transcriptional profiles upon overexpression knock-out or wild-type isogenic cell backgrounds. Targeted metabolomics provided a fingerprint 189 intracellular metabolites, while transcriptomics...
Host factor requirements for different classes of viruses have not been fully unraveled. Replication the viral genome and synthesis proteins within human host cell are associated with an increased demand nutrients specific metabolites. With more than 400 acknowledged members to date in humans, solute carriers (SLCs) represent largest family transmembrane dedicated transport ions small molecules such as amino acids, sugars nucleotides. Consistent their impact on cellular metabolism, several...
Creatine is an essential metabolite for the storage and rapid supply of energy in muscle nerve cells. In humans, impaired metabolism, transport, distribution creatine throughout tissues can cause varying forms mental disability, also known as deficiency syndrome (CDS). So far, 80 mutations transporter (SLC6A8) have been associated to CDS. To better understand effect human genetic variants on physiology SLC6A8 their possible impact CDS, we studied 30 missense including 15 unknown...
Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in variety subcellular membranes. Loss-of-function an unusually high proportion SLC is genetically associated with plethora human diseases, making SLCs rapidly emerging but challenging drug target class. Knowledge their environment may elucidate the molecular basis for functional integration...
Solute Carriers (SLCs) represent the largest family of human transporter proteins, consisting more than 400 members 1,2 . Despite importance these proteins in determining metabolic states and adaptation to environmental changes, a large proportion them is still orphan lacks associated substrates 1,3,4 Here we describe systematic mapping genetic interactions among SLCs cells. Network-based identification correlated interaction profile neighborhoods resulted initial functional assignments...
Abstract The activity and potency of a drug is inherently affected by the metabolic state its target cell. Solute Carriers (SLCs) represent largest family transmembrane transporters in humans constitute major determinants cellular metabolism. Several SLCs have been shown to be required for uptake individual chemical compounds into systems, but systematic surveys transporter-drug relationships human cells are currently lacking. We performed series genetic screens haploid cell line HAP1 using...
Abstract Host factor requirements for different classes of viruses are still to be fully unraveled. Replication the viral genome and synthesis proteins inside human host cell associated with altered, often enhanced, cellular metabolism increased demand nutrients as well specific metabolites. With more than 400 members listed date in humans, solute carriers (SLCs) represent largest family transmembrane dedicated transport ions small molecules such amino acids, sugars nucleotides. Consistent...