A5001854753- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Light effects on plants
- Plant nutrient uptake and metabolism
- Hydrocarbon exploration and reservoir analysis
- Digital Economy and Work Transformation
- Maritime Ports and Logistics
- Climate variability and models
- Methane Hydrates and Related Phenomena
- Advanced Polymer Synthesis and Characterization
- Polysaccharides and Plant Cell Walls
- Toxic Organic Pollutants Impact
- Labor market dynamics and wage inequality
- Coal Properties and Utilization
- Transgenic Plants and Applications
- Coal and Its By-products
- Liquid Crystal Research Advancements
- Food Allergy and Anaphylaxis Research
- Geology and Paleoclimatology Research
- Analytical chemistry methods development
- Meteorological Phenomena and Simulations
- Agricultural pest management studies
- Analytical Chemistry and Chromatography
Nagoya University
2015-2025
Fukuoka Institute of Health and Environmental Sciences
2008-2025
Kanagawa Dental University
2002-2024
Kyoei University
2023
Japan Institute for Labour Policy and Training
2016-2022
Geological Survey of Japan
2017-2022
National Institute of Advanced Industrial Science and Technology
2017-2021
ORCID
2021
Sage Therapeutics (United States)
2021
Nagoya City University
2018
Abstract The phytohormone auxin is a major regulator of diverse aspects plant growth and development. ubiquitin-ligase complex SCFTIR1/AFB (for Skp1-Cul1-F-box protein), which includes the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX (TIR1/AFB) receptor family, has recently been demonstrated to be critical for auxin-mediated transcriptional regulation. Early-phase auxin-induced hypocotyl elongation, on other hand, long explained by acid-growth theory, proton extrusion plasma membrane...
Abstract The phytohormone auxin controls many processes in plants, at least part through its regulation of cell expansion 1 . acid growth hypothesis has been proposed to explain auxin-stimulated for five decades, but the mechanism that underlies auxin-induced cell-wall acidification is poorly characterized. Auxin induces phosphorylation and activation plasma membrane H + -ATPase pumps protons into apoplast 2 , yet how activates remains unclear. Here we show transmembrane kinase (TMK)...
Blue light (BL) receptor phototropins activate the plasma membrane H(+)-ATPase in guard cells through phosphorylation of a penultimate threonine and subsequent binding 14-3-3 protein to phosphorylated C-terminus H⁺-ATPase, mediating stomatal opening. To date, detection level cell H⁺-ATPase has been performed biochemically using protoplasts (GCPs). However, preparation GCPs from Arabidopsis for this purpose requires >5,000 rosette leaves takes >8 h. Here, we show that BL-induced is detected...
Plasma membrane H(+)-ATPase is thought to mediate hypocotyl elongation, which induced by the phytohormone auxin through phosphorylation of penultimate threonine H(+)-ATPase. However, regulation during elongation other signals has not been elucidated. Hypocotyl in etiolated seedlings Arabidopsis thaliana was suppressed inhibitors vanadate and erythrosine B, significantly reduced aha2-5, a knockout mutant major isoform seedlings. Application ABA within 30 min at half-inhibitory concentration...
Abstract Plasma membrane (PM) H + -ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) required enzyme activation. In this study, comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red blue light increase the Thr881, PM -ATPase. Light-induced in conductance are reduced transgenic plants expressing mutant AHA1-T881A aha1–9 , whereas pen-Thr...
Mg-chelatase H subunit (CHLH) is a multifunctional protein involved in chlorophyll synthesis, plastid-to-nucleus retrograde signaling, and ABA perception. However, whether CHLH acts as an actual receptor remains controversial. Here we present evidence that affects signaling stomatal guard cells but not itself receptor. We screened ethyl methanesulfonate-treated Arabidopsis thaliana plants with focus on aperture-dependent water loss detached leaves isolated rapid transpiration 1 (rtl1) mutant...
Brassinosteroids (BRs) are steroid phytohormones that regulate plant growth and development, promote cell elongation at least in part via the acid-growth process. BRs have been suggested to induce by activating plasma membrane (PM) H+-ATPase. However, mechanism which activate PM H+-ATPase has not clarified. In this study, we investigated effects of BR on hypocotyl phosphorylation status a penultimate residue, threonine, H+-ATPase, affects activation, etiolated seedlings Arabidopsis thaliana....
Abstract The auxin-inducible degron (AID) system enables rapid depletion of target proteins within the cell by applying natural auxin IAA. AID is useful for investigating physiological functions essential proteins; however, this generally requires high dose to achieve effective in vertebrate cells. Here, we describe a super-sensitive that incorporates synthetic derivative 5-Ad-IAA and its high-affinity-binding partner OsTIR1F74A. enabled more than 1000-fold reduction inducer concentrations...
Plant cell growth requires the elongation of cells mediated by wall remodelling and turgor pressure changes. The plasma membrane (PM) H+-ATPase facilitates both loosening changes acidifying apoplast cells, referred to as acid growth. theory is mostly established on auxin-induced activation PM in non-photosynthetic tissues. However, how affects photosynthetic tissues Arabidopsis remains unclear. Here, a combination transcriptomics cis-regulatory element analysis was conducted identify impact...
Stomatal movements are regulated by multiple environmental signals. Recent investigations indicate that photoperiodic flowering components, such as CRY, GI, CO, FT and TSF, expressed in guard cells positively affect stomatal opening Arabidopsis thaliana. Here we show SOC1, which encodes a MADS box transcription factor integrates signals, also exerts positive effect on opening. FLC potent repressor of FRI acts an activator FLC. Thus, examined phenotypes FRI-Col, contains active allele...
Abstract Stomatal pores in the plant epidermis open and close to regulate gas exchange between leaves atmosphere. Upon light stimulation, plasma membrane (PM) H + -ATPase is phosphorylated activated via an intracellular signal transduction pathway stomatal guard cells, providing a primary driving force for opening movement. To uncover manipulate this pathway, we screened chemical library identified benzyl isothiocyanate (BITC), Brassicales-specific metabolite, as potent stomatal-opening...
The plant plasma membrane (PM) H+-ATPase regulates pH homeostasis and cell elongation in roots through the formation of an electrochemical H+ gradient across PM a decrease apoplastic pH; however, detailed signaling for regulation H+-ATPases remains unclear. Here, we show that auxin influx carrier, AUXIN RESISTANT1 (AUX1), is required maintenance activity proper root elongation. We isolated low pH-hypersensitive 1 (loph1) mutant by genetic screen Arabidopsis thaliana on agar plates. loph1...
The Indian subcontinent suffered from a severe drought during the summer monsoon season of 2009 when El Niño‐like conditions evolved in Pacific. warm sea surface temperature (SST) anomalies this were seen throughout tropical Pacific and over subtropical northwest Since atmospheric heat sources perturbed somewhat differently abnormal Niño year, we have carried out three different sensitivity experiments using an Atmospheric General Circulation Model (AGCM) to understand associated...
Abstract The plasma membrane H+-ATPase generates an electrochemical gradient of H+ across the that provides driving force for solute transport and regulates pH homeostasis potential in plant cells. Recent studies have demonstrated phosphorylation penultimate threonine subsequent binding a 14-3-3 protein is major common activation mechanism vascular plants. However, there very little information on nonvascular bryophytes. Here, we show liverwort Marchantia polymorpha, which most basal lineage...
Hypoosmotic shock induced a transient increase in cytosolic free calcium concentration ([Ca 2+ ] cyt ) and subsequent activation of 50‐, 75‐ 80‐kDa protein kinases tobacco ( Nicotiana tabacum L.) suspension culture cells. Depletion external suppressed both the elevation [Ca response to hypoosmotic shock, indicating that is prerequisite for kinases. Pharmacological analysis indicated shock‐activated were activated by phosphorylation, suggesting activities these are regulated putative These...
Abstract Aim: Prenatal exposure to dioxins may result in many adverse health effects. However, the mechanisms by which are transferred from mother fetus through placenta not well understood. The aim of this study was investigate differences dioxin concentrations between maternal blood, placenta, and cord blood normal pregnant women, identify individual congeners these compounds placenta. Material Methods: Samples were collected 19 Japanese women. Specific seven polychlorinated dibenzo‐ p...