- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Plant nutrient uptake and metabolism
- Allelopathy and phytotoxic interactions
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant tissue culture and regeneration
- Genomics, phytochemicals, and oxidative stress
- Transgenic Plants and Applications
- Plant Pathogens and Resistance
- Toxin Mechanisms and Immunotoxins
- Seaweed-derived Bioactive Compounds
- Collagen: Extraction and Characterization
- Plant Gene Expression Analysis
- Light effects on plants
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Protein Hydrolysis and Bioactive Peptides
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Polysaccharides and Plant Cell Walls
Okayama University
2012-2025
Chongqing University of Technology
2024-2025
Shanghai Jiao Tong University
2010-2024
Peking University
2022-2024
Shanghai Ninth People's Hospital
2010-2024
National Clinical Research Center for Digestive Diseases
2024
Shanghai Stomatological Hospital
2024
Nagoya University
2020-2023
Anhui University of Finance and Economics
2023
State Forestry and Grassland Administration
2022
Abstract Nitrogen (N) and carbon (C) are essential elements for plant growth crop yield. Thus, improved N C utilisation contributes to agricultural productivity reduces the need fertilisation. In present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H + -ATPase 1 ( OSA1 ), facilitates ammonium absorption assimilation in roots enhanced light-induced stomatal opening with higher photosynthesis rate leaves. As result, plants causes 33% increase...
Abstract Phospholipase D (PLD) is involved in responses to abiotic stress and abscisic acid (ABA) signaling. To investigate the roles of two Arabidopsis (Arabidopsis thaliana) PLDs, PLDα1 PLDδ, ABA signaling guard cells, we analyzed cells using wild type, pldα1 pldδ single mutants, a double mutant. ABA-induced stomatal closure was suppressed mutant but not pld mutants. The mutations reduced phosphatidic production epidermal tissues. Expression either or PLDδ complemented phenotype. both...
Significance Fungal disease is a major threat to agriculture and consequently human nutrition. Many pathogenic fungi penetrate through stomata. This study reveals that chitin oligosaccharide (CTOS) from fungal cell wall induces stomatal closure its receptor CERK1, Ca 2+ , S-type anion channel SLAC1, which would prevent penetration. It also shown conversion of CTOS chitosan (CSOS) possible strategy circumvent immunity. At higher concentration, guard cells perceive CSOS, resulting in death,...
Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), ethyl thiocyanate (ESCN) Arabidopsis. AITC, 3BN, ESCN induced closure wild type atrbohD atrbohF mutant. Stomatal was inhibited by catalase salicylhydroxamic acid (SHAM). The extracellular reactive oxygen species (ROS) production rosette leaves, intracellular ROS accumulation, NO...
Abstract Stomatal pores in the plant epidermis open and close to regulate gas exchange between leaves atmosphere. Upon light stimulation, plasma membrane (PM) H + -ATPase is phosphorylated activated via an intracellular signal transduction pathway stomatal guard cells, providing a primary driving force for opening movement. To uncover manipulate this pathway, we screened chemical library identified benzyl isothiocyanate (BITC), Brassicales-specific metabolite, as potent stomatal-opening...
Halitosis is a common oral condition, which leads to social embarrassment and affects quality of life. Cumulative evidence has suggested the association tongue-coating microbiome with development intraoral halitosis. The dynamic variations microbiota metabolites in halitosis have not been fully elucidated. Therefore, present study aimed determine microbial metabolic characteristics subjects without other diseases using metagenomics metabolomics analysis. participants underwent examination,...
Yeast elicitor (YEL) induces stomatal closure that is mediated by a Ca(2+)-dependent signaling pathway. A protein kinase, CPK6, positively regulates activation of ion channels in abscisic acid and methyl jasmonate signaling, leading to Arabidopsis (Arabidopsis thaliana). YEL also inhibits light-induced opening. However, it remains unknown whether CPK6 involved induction or inhibition In this study, we investigated the roles opening Arabidopsis. Disruption gene impaired Activation...
Abstract Abscisic acid (ABA) induces stomatal closure and inhibits light-induced opening. The mechanisms in these two processes are not necessarily the same. It has been postulated that ABA receptors involved opening inhibition different from those induction. Here, we provide evidence four recently identified (PYRABACTIN RESISTANCE1 [PYR1], PYRABACTIN RESISTANCE-LIKE1 [PYL1], PYL2, PYL4) sufficient for Arabidopsis (Arabidopsis thaliana). ABA-induced was impaired pyr1/pyl1/pyl2/pyl4 quadruple...
We have demonstrated that reactive carbonyl species (RCS) function as an intermediate downstream of hydrogen peroxide (H2O2) production in abscisic acid (ABA) signaling for stomatal closure guard cells using transgenic tobacco plants overexpressing alkenal reductase. investigated the conversion RCS into events cells. Both ABA and H2O2 induced RCS, such acrolein 4-hydroxy-(E)-2-nonenal (HNE), epidermal tissues wild-type Arabidopsis thaliana plants. Application scavengers, carnosine...
Drought is responsible for a massive reduction in crop yields. In response to drought, plants synthesize the hormone ABA, which induces stomatal closure, thus reducing water loss. guard cells, ABA triggers production of reactive oxygen species (ROS), mediated by NAD(P)H oxidases. The ROS key factor ABA-induced but it remains be clarified how transduced into downstream signaling components cells. We investigated roles carbonyl (RCS) closure using transgenic tobacco (Nicotiana tabacum)...
Abstract Methyl jasmonate (Me JA ) and abscisic acid ( ABA signalling cascades share several components in guard cells. We previously showed that two cell‐preferential mitogen‐activated protein kinases MAPK s), MPK 9 12 , positively regulate Arabidopsis thaliana . In this study, we examined whether these MAP function Me using genetic mutants for combined with a pharmacological approach. induced stomatal closure mpk9‐1 mpk12‐1 single as well wild‐type plants, but not double mutants....
Plasmopara viticola, the causal organism of grapevine downy mildew, secretes a vast array effectors to manipulate host immunity. Previously, several cell death-inducing PvRXLR have been identified, but their functions and targets are poorly understood. Here, we investigated role PvRXLR111, RXLR effector, in manipulating plant When coexpressed with other effectors, PvRXLR111-induced death was prevented. Transient expression PvRXLR111 Nicotiana benthamiana suppressed bacterial flagellin...
Summary Existing CRISPR/Cas12a‐based diagnostic platforms offer accurate and vigorous monitoring of nucleic acid targets, but have the potential to be further optimized for more efficient detection. Here, we profiled 16 Cas12a orthologs, focusing on their trans ‐cleavage activity as enzymes. We observed Mb2Cas12a has robust than other especially at lower temperatures. An engineered Mb2Cas12a‐RRVRR variant presented looser PAM constraints. Moreover, found existing one‐pot assay, which...
Successful expansion of hematopoietic stem cells would benefit the use cell transplants in clinic. Several angiopoietin-like proteins, including 7, can support activity cells. However, effects ANGPTL7 on human and downstream signaling cascade activated by are poorly understood. Here, we established a progenitor cell-supportive mouse fetal liver line that specifically expressed Angptl7 protein. Furthermore, found is capable stimulating increasing repopulation activities progenitors...
Production of reactive oxygen species (ROS) is a key signal event for methyl jasmonate (MeJA)- and abscisic acid (ABA)-induced stomatal closure. We recently showed that carbonyl (RCS) stimulates closure as an intermediate downstream hydrogen peroxide (H2O2) production in the ABA signaling pathway guard cells Nicotiana tabacum Arabidopsis thaliana. In this study, we examined whether RCS functions H2O2 MeJA using transgenic tobacco plants overexpressing A. thaliana 2-alkenal reductase...
Summary The oomycete pathogen Hyaloperonospora arabidopsidis delivers diverse effector proteins into host plant cells to suppress the plant’s innate immunity. In this study, we investigate mechanism of action a conserved RxLR effector, HaRxLL470, in suppressing Genomic, molecular and biochemical analyses were performed function HaRxLL470 interaction between target protein during H. infection. We report that enhances susceptibility isolate Noco2 by interacting with photomorphogenesis...