A5075899066- Cellular transport and secretion
- Plant Reproductive Biology
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Genetics, Bioinformatics, and Biomedical Research
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- Biochemical and Structural Characterization
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Protein Structure and Dynamics
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Legume Nitrogen Fixing Symbiosis
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Electrochemical Analysis and Applications
- Plant nutrient uptake and metabolism
- Advanced Fluorescence Microscopy Techniques
- Animal Genetics and Reproduction
- Corrosion Behavior and Inhibition
- Bacteriophages and microbial interactions
- Membrane-based Ion Separation Techniques
- Amino Acid Enzymes and Metabolism
Institute of Science and Technology Austria
2018-2025
Medical University of Vienna
2023-2024
Ludwig Boltzmann Cluster for Cardiovascular Research
2024
University of Exeter
2024
United States Naval Research Laboratory
2024
University of Minnesota Medical Center
2024
University of Minnesota
2023
St. Luke's University Health Network
2022
Centre National de la Recherche Scientifique
2015-2021
Institut de Biologie Intégrative de la Cellule
2015-2021
Abstract Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half wavelength light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due plants’ challenging properties. Expansion microscopy improves effective by isotropically increasing physical distances between sample structures while preserving relative arrangements and clearing sample. However, its application plants has been hindered rigid,...
Brassinosteroids are plant steroid hormones that control many aspects of growth and development, perceived at the cell surface by plasma membrane-localized receptor kinase BRI1. Here we show BRI1 is post-translationally modified K63 polyubiquitin chains in vivo. Using both artificial ubiquitination generation an ubiquitination-defective mutant form, demonstrate promotes internalization from essential for its recognition trans-Golgi network/early endosomes (TGN/EE) vacuolar targeting....
In plants, clathrin mediated endocytosis (CME) represents the major route for cargo internalisation from cell surface. It has been assumed to operate in an evolutionary conserved manner as yeast and animals. Here we report characterisation of ultrastructure, dynamics mechanisms plant CME allowed by our advancement electron microscopy quantitative live imaging techniques. Arabidopsis appears follow constant curvature model bona fide population generates vesicles a predominantly...
Article5 January 2021Open Access Source DataTransparent process Modulation of plant root growth by nitrogen source-defined regulation polar auxin transport Krisztina Ötvös orcid.org/0000-0002-5503-4983 Institute Science and Technology (IST) Austria, Klosterneuburg, Austria Bioresources Unit, Center for Health & Bioresources, AIT Austrian GmbH, Tulln, Search more papers this author Marco Marconi orcid.org/0000-0002-3457-1384 Centro de Biotecnología y Genómica Plantas (CBGP, UPM-INIA)...
In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from cell surface as well movement cargo in post-Golgi trafficking pathways. This diversity functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates part secretory pathway at trans-Golgi network (TGN), while AP-2 TPLATE jointly operate plasma membrane to execute...
Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception importance spatially heterogeneous distribution continue be debated. Here we show that by plasma membrane is an effective additional path for response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches...
The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis polar distribution PIN transporters remains limited due to contradictory observations interpretations. Here, we used state-of-the-art methods reexamine the effects endocytic trafficking. We high concentrations or longer treatments versus lower shorter natural indole-3-acetic acid (IAA) synthetic naphthalene acetic (NAA)...
Polar subcellular localization of the PIN exporters phytohormone auxin is a key determinant directional, intercellular transport and thus central topic both plant cell developmental biology. Arabidopsis mutants lacking PID, kinase that phosphorylates PINs, or MAB4/MEL proteins unknown molecular function display polarity defects phenocopy pin mutants, but mechanistic insights into how these factors convey are missing. Here, by combining protein biochemistry with quantitative live-cell...
The polybiquitination of proteins can take on different topologies depending the residue from ubiquitin involved in chain formation. Although role lysine-48 (K48) polyubiquitination proteasome-mediated degradation is fairly well characterized, much less understood about other types chains and proteasome-independent functions. To overcome this, we developed a K63 polyubiquitin-specific sensor-based approach to track isolate polyubiquitinated plants. Proteins carrying polyubiquitin were found...
Endocytosis is a key process in the internalization of extracellular materials and plasma membrane proteins, such as receptors transporters, thereby controlling many aspects cell signaling cellular homeostasis. plants has an essential role not only for basic functions but also growth development, nutrient delivery, toxin avoidance, pathogen defense. The precise mechanisms endocytosis remain quite elusive. lack direct visualization examination single events greatly hampered our ability to...
The lack of resolution when studying the many different ubiquitin chain types found in eukaryotic cells has been a major hurdle to our understanding their specific roles. We currently have very little insight into cellular and physiological functions Lys-63 (K63)–linked chains, although they are second most abundant forms plant cells. To overcome this problem, we developed several large-scale approaches characterize (1) E2-E3 ubiquitination machinery driving K63-linked formation (2) K63...
Abstract Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles via recognition of motifs based on Tyr or di-Leu in their cytoplasmic tails. However, plants, very little is known about how PM proteins sorted for CME whether similar required. Arabidopsis (Arabidopsis thaliana), brassinosteroid (BR) receptor BR...
Significance Endocytosis transports cargos inside the cell by creating spherical vesicles from plasma membrane. This membrane remodeling requires proteins to generate force bend inwards, overcoming high-turgor pressure in plant cells. However, as plants create clathrin-coated without actin, machinery membranes during endocytosis is entirely unknown and appears distinct other model systems. Here, we refine physiological role of plant-specific essential endocytic TPLATE complex. We find it...
The TPLATE complex (TPC) is a key endocytic adaptor protein in plants. TPC Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific (AtEH1/Pan1 AtEH2/Pan1) which, although cytoplasmic proteins, are not associated with the hexameric subcomplex cytoplasm. To investigate dynamic assembly of octameric at plasma membrane (PM), we performed state-of-the-art dual-color live-cell imaging physiological lowered temperatures. Lowering temperature slowed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Electrical Conductance of Several Salts in PyridineJ. H. MathewsJ. MathewsMore by J. Mathews and A. JohnsonA. JohnsonMore JohnsonCite this: Phys. Chem. 1917, 21, 4, 294–310Publication Date (Print):April 1, 1917Publication History Published online1 May 2002Published inissue 1 April 1917https://doi.org/10.1021/j150175a003RIGHTS & PERMISSIONSArticle Views37Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum...
Abstract Super-resolution methods enable spatial resolution far better than the optical diffraction limit of about half wavelength light (∼200-300 nm) but have yet to attain widespread use in plants, owing large part plants’ challenging properties. Expansion microscopy improves effective by isotropically increasing physical distances between sample structures while preserving relative arrangements, and clears sample. However, its application plants has been hindered rigid, mechanically...