A5077928039- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Trypanosoma species research and implications
- Fetal and Pediatric Neurological Disorders
- Zebrafish Biomedical Research Applications
- Environmental Monitoring and Data Management
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Photosynthetic Processes and Mechanisms
- Optical Coherence Tomography Applications
- Research on Leishmaniasis Studies
- Breast Lesions and Carcinomas
- Anesthesia and Neurotoxicity Research
- Axon Guidance and Neuronal Signaling
- Protist diversity and phylogeny
- RNA modifications and cancer
- RNA Research and Splicing
- Parasite Biology and Host Interactions
- Radiation Therapy and Dosimetry
- 3D Printing in Biomedical Research
Institute of Science and Technology Austria
2024-2025
Universidad de Los Andes
2017-2021
Universidad de Los Andes
2019-2020
Abstract Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half wavelength light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due plants’ challenging properties. Expansion microscopy improves effective by isotropically increasing physical distances between sample structures while preserving relative arrangements and clearing sample. However, its application plants has been hindered rigid,...
Abstract The information-processing capability of the brain’s cellular network depends on physical wiring pattern between neurons and their molecular functional characteristics. Mapping resolving individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution with dense labeling. Light microscopy is uniquely positioned to visualize specific molecules but dense, synapse-level circuit reconstruction light has been out reach due limitations in resolution, contrast,...
Abstract The information-processing capability of the brain’s cellular network depends on physical wiring pattern between neurons and their molecular functional characteristics. Mapping resolving individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution 1,2 with dense labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction light has been out reach, owing limitations in resolution,...
Glioblastoma (GBM) is the most prevalent type of primary brain tumor. Treatment options include maximal surgical resection and drug-radiotherapy combination. However, patient prognosis remains very poor, prompting search for new models drug discovery testing, especially those that allow assessment in vivo responses to treatment. Zebrafish xenograft have an enormous potential study tumor behavior, proliferation cellular interactions. Here, imaging method human GBM zebrafish larvae introduced....
Abstract Super-resolution methods enable spatial resolution far better than the optical diffraction limit of about half wavelength light (∼200-300 nm) but have yet to attain widespread use in plants, owing large part plants’ challenging properties. Expansion microscopy improves effective by isotropically increasing physical distances between sample structures while preserving relative arrangements, and clears sample. However, its application plants has been hindered rigid, mechanically...
Super-resolution microscopy often entails long acquisition times of minutes to hours. Since drifts during the adversely affect data quality, active sample stabilization is commonly used for some these techniques reach their full potential. While in lateral plane can be corrected after acquisition, this not always possible or may come with drawbacks. Therefore, it appealing stabilize position three dimensions acquisition. Various schemes have been demonstrated previously, reaching sub-nm...
Chagas disease is a parasitic infection caused by Trypanosoma cruzi, whose motility not only important for localization, but also cellular binding and invasion. Current animal models the study of T. cruzi allow limited observation parasites in vivo, representing challenge understanding parasite behavior during initial stages humans. This protozoan has flagellar stage both vector mammalian hosts, there are no studies describing its vivo.The objective this project was to establish live...
Abstract Objective F-spondin is part of a group evolutionarily conserved extracellular matrix proteins in vertebrates. It highly expressed the embryonic floor plate, and it can bind to ECM promote neuronal outgrowth. A characterization expression patterns adult zebrafish brain was previously reported by our group. However, given its importance during development, we aimed obtain detailed description green fluorescent protein (GFP) driven spon1b promotor, developing transgenic Tg(spon1b:GFP)...
Chagas disease is a parasitic infection caused by Trypanosoma cruzi, whose motility not only important for localization, but also cellular binding and invasion. Current animal models the study of T. cruzi allow limited observation parasites in vivo, representing challenge understanding parasite behavior during initial stages humans. This protozoan has flagellar stage both vector mammalian hosts, there are no studies describing its vivo.The objective this project was to establish live...