A5062986713- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Cardiomyopathy and Myosin Studies
- Force Microscopy Techniques and Applications
- RNA Research and Splicing
- Crystallography and molecular interactions
- Optical Coherence Tomography Applications
- CAR-T cell therapy research
- Catalysis for Biomass Conversion
- Polyoxometalates: Synthesis and Applications
- Chemical Synthesis and Reactions
- Energetic Materials and Combustion
Institute of Science and Technology Austria
2022-2025
Manipal Academy of Higher Education
2019-2020
Institute for Stem Cell Biology and Regenerative Medicine
2019-2020
Indian Institute of Science Education and Research, Bhopal
2020
National Institute of Technology Karnataka
2017-2019
Resource22 June 2020Open Access Source DataTransparent process Structural insights into actin filament recognition by commonly used cellular markers Archana Kumari Center for Cardiovascular Biology and Diseases, Institute Stem Cell Science Regenerative Medicine, Bengaluru, India Search more papers this author Shubham Kesarwani Manipal Academy of Higher Education, Manipal, Manjunath G Javoor Kutti R Vinothkumar Corresponding Author [email protected] National Biological Sciences, TIFR,...
Regulation of the Arp2/3 complex is required for productive nucleation branched actin networks. An emerging aspect regulation incorporation subunit isoforms into complex. Specifically, both ArpC5 isoforms, and ArpC5L, have been reported to fine-tune activity branch junction stability. We combined reverse genetics cellular structural biology describe how ArpC5L differentially affect cell migration. Both define stability ArpC1 in junctions and, turn, by determining protrusion characteristics,...
Super-resolution microscopy often entails long acquisition times of minutes to hours. Since drifts during the adversely affect data quality, active sample stabilization is commonly used for some these techniques reach their full potential. While in lateral plane can be corrected after acquisition, this not always possible or may come with drawbacks. Therefore, it appealing stabilize position three dimensions acquisition. Various schemes have been demonstrated previously, reaching sub-nm...
Abstract This work reports a straightforward and scalable synthesis of series alkyl levulinates from cellulose‐derived levulinic acid alcohols using commercially available heteropolyacid catalysts under homegenous conditions. The reaction was optimized on parameters such as temperature, molar ratio reagents, type loading catalyst. solvent‐free reactions afforded in high isolated yields (>85%) only slight excess 10 wt% catalyst at 120 °C 6 h. Further, the were successfully recycled for...
Crystalline forms of 5-fluoroisatin have been characterized by thermal, structural, and mechanical methods. The hardness elastic modulus the DMSO solvate is significantly higher than those picolinic acid co-crystal 5-fluoroisatin.
Abstract Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions toxins, peptides and proteins that bind actin. While the choice these markers has been largely based on availability ease, there is a severe dearth structural data for an informed judgment in employing suitable F-actin particular requirement. Here we describe electron cryomicroscopy structures phalloidin, lifeAct utrophin bound to F-actin, providing first high-resolution comparison widely...
Abstract Tight regulation of Arp2/3 complex is required to allow productive nucleation force-generating, branched actin networks. An emerging aspect the incorporation subunit isoforms into complex. Specifically, both ArpC5 subunit, and ArpC5L, have been reported fine-tune activity branch junction stability. Elevated levels also linked increased cancer progression metastasis. Here, we combined genetic engineering cells cellular structural biology describe how ArpC5L differentially regulate...