A5036888639- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Cellular transport and secretion
- Genomic variations and chromosomal abnormalities
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
- Marine Biology and Environmental Chemistry
- RNA Interference and Gene Delivery
- Environmental Toxicology and Ecotoxicology
- Protein Degradation and Inhibitors
Osaka University
2016-2025
Ube Frontier University
2018-2024
National Institute of Genetics
2009-2020
France Business School
2016-2019
The Graduate University for Advanced Studies, SOKENDAI
2006-2016
Frontier Science Foundation
2016
University of Oxford
1997-2013
Kobe University
2013
Erasmus MC
2013
Kyoto University
2013
Regulated interactions between kinetochores and spindle microtubules are essential to maintain genomic stability during chromosome segregation. The Aurora B kinase phosphorylates kinetochore substrates destabilize kinetochore–microtubule eliminate incorrect attachments. These must be dephosphorylated stabilize correct attachments, but how opposing phosphatase activities coordinated at the is unknown. Here, we demonstrate that a conserved motif in protein KNL1 directly interacts with targets...
Despite many decades of study, mitotic chromosome structure and composition remain poorly characterized. Here, we have integrated quantitative proteomics with bioinformatic analysis to generate a series independent classifiers that describe the approximately 4,000 proteins identified in isolated chromosomes. Integrating these by machine learning uncovers functional relationships between protein complexes context intact chromosomes reveals which 560 uncharacterized here merits further study....
Microchromosomes are prevalent in nonmammalian vertebrates [P. D. Waters et al. , Proc. Natl. Acad. Sci. U.S.A. 118 (2021)], but a few of them missing bird genome assemblies. Here, we present new chicken reference containing all autosomes, Z and W chromosome, with gaps closed except for the W. We identified ten small microchromosomes (termed dot chromosomes) distinct sequence epigenetic features, among which six were newly assembled. Those chromosomes exhibit extremely high GC content level...
Centromeres are scaffolds for the assembly of kinetochores that ensure chromosome segregation during cell division. How vertebrate centromeres obtain a three-dimensional structure to accomplish their primary function is unclear. Using super-resolution imaging, capture-C, and polymer modeling, we show partitioned by condensins into two subdomains mitosis. The bipartite found in human, mouse, chicken cells therefore fundamental feature centromeres. Super-resolution imaging electron tomography...
During cell division, kinetochores form the primary chromosomal attachment sites for spindle microtubules. We previously identified a network of 10 interacting kinetochore proteins conserved between Caenorhabditis elegans and humans. In this study, we investigate three in human (hDsn1Q9H410, hNnf1PMF1, hNsl1DC31). Using coexpression bacteria fractionation mitotic extracts, demonstrate that these stable complex with component hMis12. Human or chicken cells depleted Mis12 subunits are delayed...
A longstanding question in centromere biology has been the organization of CENP-A–containing chromatin and its implications for kinetochore assembly. Here, we have combined genetic manipulations with deconvolution super-resolution fluorescence microscopy a detailed structural analysis chicken kinetochores. Using subdiffraction spatial resolution single molecule sensitivity to map protein localization unfolded by exposure low salt buffer, observed robust amounts H3K9me3, but only levels...
CENP-A acts as an important epigenetic marker for kinetochore specification. However, the mechanisms by which is incorporated into centromeres and structural basis formation downstream of remain unclear. Here, we used a unique chromosome-engineering system in proteins are targeted to noncentromeric site after endogenous centromere conditionally removed. Using this system, created two distinct types engineered kinetochores, both were stably maintained chicken DT40 cells. Ectopic targeting...
Chromosome segregation during mitosis requires the assembly of a large proteinaceous structure termed kinetochore. In Caenorhabditis elegans, KNL-1 is required to target multiple outer kinetochore proteins. Here, we demonstrate that vertebrate KNL1 counterpart essential for chromosome and localize subset However, unlike in C. depletion does not abolish localization microtubule-binding Ndc80 complex. Instead, show CENP-K, subunit constitutively centromere-associated complex missing from...
CENP-A is an evolutionarily conserved, centromere-specific variant of histone H3 that thought to play a central role in directing kinetochore assembly and centromere function. Here, we have analyzed the consequences disrupting gene chicken DT40 cell line. In CENP-A-depleted cells, protein impaired, as indicated by mislocalization inner proteins CENP-I, CENP-H, CENP-C well outer components Nuf2/Hec1, Mad2, CENP-E. However, BubR1 INCENP are efficiently recruited kinetochores. Following...
The centromere is essential for faithful chromosome segregation by providing the site kinetochore assembly. Although role of conserved throughout evolution, DNA sequences associated with regions are highly divergent among species and it remains to be determined how directs formation. Despite active use chicken DT40 cells in studies segregation, sequence was unclear. Here, we performed a comprehensive analysis which revealed unique features centromeres compared previously studied vertebrates....
Centromeres are specified by sequence-independent epigenetic mechanisms in most organisms. Rarely, centromere repositioning results neocentromere formation at ectopic sites. However, the governing how and where neocentromeres form unknown. Here, we established a chromosome-engineering system chicken DT40 cells that allowed us to efficiently isolate neocentromere-containing chromosomes. Neocentromeres appear be structurally functionally equivalent native centromeres. Chromatin...