A5001883766- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Nuclear Structure and Function
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Nematode management and characterization studies
- Toxoplasma gondii Research Studies
- Cell Image Analysis Techniques
- Fungal and yeast genetics research
- Plant responses to water stress
- Endoplasmic Reticulum Stress and Disease
- Parasitic Infections and Diagnostics
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Image Processing Techniques and Applications
- Cannabis and Cannabinoid Research
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Amoebic Infections and Treatments
- Advanced Fluorescence Microscopy Techniques
- RNA regulation and disease
Centre National de la Recherche Scientifique
2007-2023
Institut de Biologie Paris-Seine
2015-2023
Laboratoire de Biologie du Développement
2010-2023
Sorbonne Université
2010-2023
Université Paris Cité
2012
European Molecular Biology Organization
2002-2008
Institut Pasteur
2000-2007
European Molecular Biology Laboratory
2006-2007
European Molecular Biology Laboratory
2002-2006
Inserm
2002
In sexual reproduction of most animals, the spermatozoon provides DNA and centrioles, together with some cytoplasm organelles, to oocyte that is being fertilized. Paternal mitochondria their genomes are generally eliminated in embryo by an unknown degradation mechanism. We show that, upon fertilization, a Caenorhabditis elegans triggers recruitment autophagosomes within minutes subsequent paternal degradation. Whereas nematode-specific sperm membranous organelles ubiquitinated before...
Nuclear pore complexes (NPCs) span the nuclear envelope and mediate communication between nucleus cytoplasm. To obtain insight into structure function of NPCs multicellular organisms, we have initiated an extensive analysis Caenorhabditis elegans nucleoporins. Of 20 assigned C. nucleoporin genes, 17 were found to be essential for embryonic development either alone or in combination. In several cases, depletion nucleoporins by RNAi caused severe defects appearance. More specifically, homologs...
The small GTPase Ran has been found to play pivotal roles in several aspects of cell function. We have investigated the role cycle spindle formation and nuclear envelope assembly dividing Caenorhabditis elegans embryos real time. that its cofactors RanBP2, RanGAP, RCC1 are all essential for reformation after division. Reducing expression any these components by RNAi leads strong extranuclear clustering integral proteins nucleoporins. Ran, RanGAP also required building a mitotic spindle,...
The cytoplasmic and nuclear compartments of animal cells mix during mitosis on disassembly the envelope (NE). NE breakdown (NEBD) involves dispersion membranes associated proteins, including pore complexes (NPCs) lamina. Among approximately 30 NPC components known, few contain transmembrane domains. gp210 is a single-pass glycoprotein metazoan NPCs. We show that both RNAi-mediated depletion mutation Caenorhabditis elegans affect NEBD in early embryonic cells, preventing lamin...
Proteins bearing a SET domain have been shown to methylate lysine residues in histones and contribute chromatin architecture. Methylation of histone H3 at 9 (H3K9) has emerged as an important player the formation heterochromatin, condensation, transcriptional repression. Here, we characterized previously undescribed member H3K9 methyltransferase family named CLLD8 (or SETDB2 or KMT1F). This protein contributes trimethylation both interspersed repetitive elements centromere-associated repeats...
Studying how individual cells spatially and temporally organize within the embryo is a fundamental issue in modern developmental biology to better understand first stages of embryogenesis. In order perform high-throughput analyses three-dimensional microscopic images, it essential be able automatically segment, classify track cell nuclei. Many 3D/4D segmentation tracking algorithms have been reported literature. Most them are specific particular models or acquisition systems often require...
Macroautophagic degradation of sperm-inherited organelles prevents paternal mitochondrial DNA transmission in C. elegans. The recruitment autophagy markers around sperm mitochondria has also been observed mouse and fly embryos but their role is debated. Both worm Atg8 ubiquitin-like proteins, LGG-1/GABARAP LGG-2/LC3, are recruited after fertilization. Whereas LGG-1 depletion affects autophagosome function, stabilizes the substrates lethal, we demonstrate that LGG-2 dispensable for formation...
Entamoeba histolytica migration is essential for the development of amoebiasis, a human disease characterised by invasion and destruction tissues. Amoebic motility requires both polarisation cell formation predominant pseudopod. As p21-activated kinases PAKs are known to regulate eukaryotic morphology, we investigated role PAK in E. histolytica. We showed that C-terminal domain EhPAK comprised constitutive kinase activity vitro overproduction this fragment, histolytica, caused significant...
In most animals, during oocyte fertilization the spermatozoon provides DNA and centrioles together with some cytoplasm organelles, but paternal mitochondria are generally eliminated in embryo. Using model animal C. elegans we have shown that organelle degradation is dependent on formation of autophagosomes a few minutes after fertilization. This macroautophagic process preceded by an active ubiquitination spermatozoon-inherited organelles. Analysis fertilized mouse embryos suggests this...
The uniparental transmission of the maternal mitochondrial genome is achieved by disposal sperm mitochondria and their DNA (mtDNA) around fertilization. C. elegans embryo allowed identification several factors mechanisms involved after discovery that autophagy pathway its adaptor ALLO-1 are implicated represented a major progress. In addition, studies in fish fly have CPS-6, endonuclease, degradation sperm-derived mtDNA. mitochondria-associated proteins FNDC-1 PHB-2 were also efficiently...
Loss of membrane potential sperm mitochondria has been regarded as the first step preceding mitophagy degradation after their entry into C. elegans oocyte at fertilization. This is in line with classical view defective or abnormal and could serve a recognition signal for specific quick autophagy degradation. Here, using TMRE (tetramethylrhodamine ethyl ester) live imaging we show that this not case. Instead, inherited stable labeling before time autophagosomes formation. Interestingly,...