A5103283365- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- High Altitude and Hypoxia
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Histone Deacetylase Inhibitors Research
- Congenital Anomalies and Fetal Surgery
- RNA modifications and cancer
- Ginkgo biloba and Cashew Applications
- Fire effects on ecosystems
- Visual and Cognitive Learning Processes
- Diphtheria, Corynebacterium, and Tetanus
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Breastfeeding Practices and Influences
- Biochemical and Structural Characterization
- Cholangiocarcinoma and Gallbladder Cancer Studies
First Affiliated Hospital of Kunming Medical University
2025
Academy of Military Medical Sciences
2020-2022
Cincinnati Children's Hospital Medical Center
2014-2019
National Vaccine and Serum Institute
2014
Southwest University
2013
Beijing Fengtai Hospital
2013
Developing therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on receptor binding domain (RBD) Spike (S) protein but also across full protein. We isolated and characterized monoclonal antibodies (mAbs) from 10 convalescent COVID-19 patients. Three mAbs showed neutralizing activities authentic SARS-CoV-2. One mAb, named 4A8, exhibits high neutralization potency both pseudotyped SARS-CoV-2 does bind RBD....
Abstract The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) presents a global public health threat. Most research on therapeutics against SARS-CoV-2 focused the receptor binding domain (RBD) Spike (S) protein, whereas vulnerable epitopes and functional mechanism non-RBD regions are poorly understood. Here we isolated characterized monoclonal antibodies (mAbs) derived from convalescent COVID-19 patients. An mAb targeting N-terminal...
Abstract The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection- and vaccination-induced antibodies, highlighting the demands for research on continuing discovery of broadly neutralizing antibodies (bnAbs). Here, we developed a panel bnAbs against other variants concern (VOCs) elicited vaccination adenovirus-vectored COVID-19 vaccine (Ad5-nCoV). We also investigated human longitudinal antibody responses following demonstrated how evolved over time. A...
Myelodysplastic syndromes (MDS) are heterogeneous hematopoietic disorders that incurable with conventional therapy. Their incidence is increasing global population aging. Although many genetic, epigenetic, splicing, and metabolic aberrations have been identified in patients MDS, their clinical features quite similar. Here, we show hypoxia-independent activation of hypoxia-inducible factor 1α (HIF1A) signaling both necessary sufficient to induce dysplastic cytopenic MDS phenotypes. The HIF1A...
SET domain containing 2 (Setd2), encoding a histone methyltransferase, is associated with many hematopoietic diseases when mutated. By generating novel exon 6 conditional knockout mouse model, we describe an essential role of Setd2 in maintaining the adult stem cells. Loss results leukopenia, anemia, and increased platelets accompanied by hypocellularity, erythroid dysplasia, mild fibrosis bone marrow. mice show significantly decreased progenitor cells except for progenitors. fail to...
The Rift Valley fever virus (RVFV) is an arthropod-borne that can not only cause severe disease in domestic animals but also humans. However, the licensed vaccines or available therapeutics for humans do exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Gn and Gc protein (rHAdV4-GnGcopt). NAbs were both able to protect host cells RVFV infection. interactions between then...
Chronic hepatitis B virus (HBV) infection is an independent risk factor for the development of hepatocellular carcinoma (HCC). The HBV HBx gene frequently identified as integrant in chromosomal DNA patients with HCC. encodes X protein (HBx), a putative viral oncoprotein that affects transcriptional regulation several cellular genes. Therefore, may be ideal target to impede progression infection-related In this study, integrated was transcriptionally downregulated using artificial...
Coronavirus disease 2019 (COVID-19) has caused over 220,000 deaths so far and is still an ongoing global health problem. However, the immunopathological changes of key types immune cells during after virus infection remain unclear. Here, we enriched CD3+ CD19+ lymphocytes from peripheral blood mononuclear COVID-19 patients (severe recovered at early or late stages) healthy people (SARS-CoV-2 negative) revealed transcriptional profiles in these by comprehensive single-cell transcriptome V(D)J...
Clostridium tetani causes life-threatening disease by producing tetanus neurotoxin (TeNT), one of the most toxic protein substances. Toxicosis can be prevented and cured administration anti-TeNT neutralizing antibodies. Here, we identified a series monoclonal antibodies (mAbs) derived from memory B cells healthy adult immunized with C-terminal domain TeNT (TeNT-Hc). Thirteen mAbs bound to both toxoid (TT) TeNT-Hc, while two recognized only TT. VH3-23 was frequently used germline gene in...